Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium
Abstract The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi‐synthetic food AIN93 were compared to mice fed AIN93‐low calcium (LC). Administration of AIN93‐LC resulted in...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-12-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.936 |
| _version_ | 1811343885228572672 |
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| author | Janetta Top Antoni P. A. Hendrickx Marleen T. J. vanAmpting Kees vanLimpt Jan Knol Denise van deKamer Johanna C. Braat Marco Viveen Malbert R. Rogers Hans Kemperman Rob J. L. Willems Fernanda L. Paganelli |
| author_facet | Janetta Top Antoni P. A. Hendrickx Marleen T. J. vanAmpting Kees vanLimpt Jan Knol Denise van deKamer Johanna C. Braat Marco Viveen Malbert R. Rogers Hans Kemperman Rob J. L. Willems Fernanda L. Paganelli |
| author_sort | Janetta Top |
| collection | DOAJ |
| description | Abstract The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi‐synthetic food AIN93 were compared to mice fed AIN93‐low calcium (LC). Administration of AIN93‐LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93‐LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen. |
| first_indexed | 2024-04-13T19:38:03Z |
| format | Article |
| id | doaj.art-99f63c69d0ac4ad8919f5042c9d162d1 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-04-13T19:38:03Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-99f63c69d0ac4ad8919f5042c9d162d12022-12-22T02:32:58ZengWileyMicrobiologyOpen2045-88272019-12-01812n/an/a10.1002/mbo3.936Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faeciumJanetta Top0Antoni P. A. Hendrickx1Marleen T. J. vanAmpting2Kees vanLimpt3Jan Knol4Denise van deKamer5Johanna C. Braat6Marco Viveen7Malbert R. Rogers8Hans Kemperman9Rob J. L. Willems10Fernanda L. Paganelli11Department of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsDanone Nutricia Research Utrecht The NetherlandsDanone Nutricia Research Utrecht The NetherlandsDanone Nutricia Research Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsClinical Chemistry and Hematology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsDepartment of Medical Microbiology University Medical Center Utrecht Utrecht The NetherlandsAbstract The aim of this study was to determine whether dietary intervention influenced luminal Ca2+ levels and Enterococcus faecium gut colonization in mice. For this purpose, mice fed semi‐synthetic food AIN93 were compared to mice fed AIN93‐low calcium (LC). Administration of AIN93‐LC resulted in lower luminal Ca2+ levels independent of the presence of E. faecium. Furthermore, E. faecium gut colonization was reduced in mice fed AIN93‐LC based on culture, and which was in concordance with a reduction of Enterococcaceae in microbiota analysis. In conclusion, diet intervention might be a strategy for controlling gut colonization of E. faecium, an important opportunistic nosocomial pathogen.https://doi.org/10.1002/mbo3.936Enterococcus faeciumgut colonizationlow‐calcium dietmouse model |
| spellingShingle | Janetta Top Antoni P. A. Hendrickx Marleen T. J. vanAmpting Kees vanLimpt Jan Knol Denise van deKamer Johanna C. Braat Marco Viveen Malbert R. Rogers Hans Kemperman Rob J. L. Willems Fernanda L. Paganelli Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium MicrobiologyOpen Enterococcus faecium gut colonization low‐calcium diet mouse model |
| title | Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium |
| title_full | Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium |
| title_fullStr | Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium |
| title_full_unstemmed | Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium |
| title_short | Low‐calcium diet in mice leads to reduced gut colonization by Enterococcus faecium |
| title_sort | low calcium diet in mice leads to reduced gut colonization by enterococcus faecium |
| topic | Enterococcus faecium gut colonization low‐calcium diet mouse model |
| url | https://doi.org/10.1002/mbo3.936 |
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