Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma
Objective:Despite all the recent advancements, larynx cancer has shown no improvement in survival rates. The aim of this study was to investigate the expressions of toll-like receptor (TLR)-2, -3, and -4 genes, and determine any relationships with the histopathologic characteristics of the disease.M...
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| Format: | Article |
| Language: | English |
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Galenos Yayincilik
2022-09-01
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| Series: | Turkish Archives of Otorhinolaryngology |
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http://turkarchotolaryngol.net/archives/archive-detail/article-preview/nvestigation-of-toll-like-receptor-2--3-and--4-gen/54634
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| author | Çağlar Eker Hakkı Caner İnan Asuman Çelebi Emine Deniz Gözen Emin Karaman |
| author_facet | Çağlar Eker Hakkı Caner İnan Asuman Çelebi Emine Deniz Gözen Emin Karaman |
| author_sort | Çağlar Eker |
| collection | DOAJ |
| description | Objective:Despite all the recent advancements, larynx cancer has shown no improvement in survival rates. The aim of this study was to investigate the expressions of toll-like receptor (TLR)-2, -3, and -4 genes, and determine any relationships with the histopathologic characteristics of the disease.Methods:This retrospective study included 50 subjects who underwent total or partial laryngectomy with an open surgical method for larynx squamous cell carcinoma. Measurements of TLRs-2, -3, and -4 expression values were taken with quantitative real time-polymerase chain reaction in normal tissue and tumor tissue samples of the patients.Results:Evaluations were made of TLR-2, -3, and -4 mRNA expressions according to 2-ΔΔCT calculations in 50 subjects with larynx cancer. When the tumor tissue was compared with the healthy tissue from the same subjects, reductions were determined in TLR expression in 86%, 84%, and 82%, respectively. This reduction in each gene expression was statistically significant (p<0.001). No statistically significant correlation was determined between the change in TLR-2, -3, and -4 expression and the histopathologic characteristics of the disease.Conclusion:The data obtained in this study demonstrated that TLR-2, -3, and -4 expressions were reduced in larynx squamous cell cancer. The results of further studies targeting these genes would be useful in the diagnosis and treatment of the disease. |
| first_indexed | 2024-04-10T14:17:39Z |
| format | Article |
| id | doaj.art-99fa6972c8594994b112ce930e46099a |
| institution | Directory Open Access Journal |
| issn | 2667-7474 |
| language | English |
| last_indexed | 2024-04-10T14:17:39Z |
| publishDate | 2022-09-01 |
| publisher | Galenos Yayincilik |
| record_format | Article |
| series | Turkish Archives of Otorhinolaryngology |
| spelling | doaj.art-99fa6972c8594994b112ce930e46099a2023-02-15T16:09:28ZengGalenos YayincilikTurkish Archives of Otorhinolaryngology2667-74742022-09-0160312112710.4274/tao.2022.2022-6-1713049054Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell CarcinomaÇağlar Eker0Hakkı Caner İnan1Asuman Çelebi2Emine Deniz Gözen3Emin Karaman4 Department of Otorhinolaryngology, İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, İstanbul, Turkey Department of Otorhinolaryngology, İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, İstanbul, Turkey Department of Medical Biology, İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, İstanbul, Turkey Department of Otorhinolaryngology, İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, İstanbul, Turkey Department of Otorhinolaryngology, İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, İstanbul, Turkey Objective:Despite all the recent advancements, larynx cancer has shown no improvement in survival rates. The aim of this study was to investigate the expressions of toll-like receptor (TLR)-2, -3, and -4 genes, and determine any relationships with the histopathologic characteristics of the disease.Methods:This retrospective study included 50 subjects who underwent total or partial laryngectomy with an open surgical method for larynx squamous cell carcinoma. Measurements of TLRs-2, -3, and -4 expression values were taken with quantitative real time-polymerase chain reaction in normal tissue and tumor tissue samples of the patients.Results:Evaluations were made of TLR-2, -3, and -4 mRNA expressions according to 2-ΔΔCT calculations in 50 subjects with larynx cancer. When the tumor tissue was compared with the healthy tissue from the same subjects, reductions were determined in TLR expression in 86%, 84%, and 82%, respectively. This reduction in each gene expression was statistically significant (p<0.001). No statistically significant correlation was determined between the change in TLR-2, -3, and -4 expression and the histopathologic characteristics of the disease.Conclusion:The data obtained in this study demonstrated that TLR-2, -3, and -4 expressions were reduced in larynx squamous cell cancer. The results of further studies targeting these genes would be useful in the diagnosis and treatment of the disease. http://turkarchotolaryngol.net/archives/archive-detail/article-preview/nvestigation-of-toll-like-receptor-2--3-and--4-gen/54634 larynx cancertoll-like receptorsgene expressionsquamous cell carcinomatumor microenvironment |
| spellingShingle | Çağlar Eker Hakkı Caner İnan Asuman Çelebi Emine Deniz Gözen Emin Karaman Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma Turkish Archives of Otorhinolaryngology larynx cancer toll-like receptors gene expression squamous cell carcinoma tumor microenvironment |
| title | Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma |
| title_full | Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma |
| title_fullStr | Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma |
| title_full_unstemmed | Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma |
| title_short | Investigation of Toll-like Receptor-2, -3 and -4 Gene Expressions in Larynx Squamous Cell Carcinoma |
| title_sort | investigation of toll like receptor 2 3 and 4 gene expressions in larynx squamous cell carcinoma |
| topic | larynx cancer toll-like receptors gene expression squamous cell carcinoma tumor microenvironment |
| url |
http://turkarchotolaryngol.net/archives/archive-detail/article-preview/nvestigation-of-toll-like-receptor-2--3-and--4-gen/54634
|
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