Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD

Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease tha...

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Main Author: chylen setiyo rini
Format: Article
Language:Indonesian
Published: Fakultas Kesehatan Universitas Nahdlatul Ulama Surabaya 2023-04-01
Series:Medical Technology and Public Health Journal
Subjects:
Online Access:https://journal2.unusa.ac.id/index.php/MTPHJ/article/view/3837
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author chylen setiyo rini
author_facet chylen setiyo rini
author_sort chylen setiyo rini
collection DOAJ
description Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease that attacks the intestines. This study aims to determine the kaffir lime leaf extract in inhibiting the growth of Bacillus cereus bacteria using the Time-Kill Test method. This study used the Minimum Inhibitory Concentration (MIC) and Time-Kill Test methods. The MIC results in kaffir lime leaf extract that can inhibit bacteria at 2 mg/mL, while the Time-Kill Test shows a death phase at 24 hours. Kaffir lime leaf extract has a bacteriostatic effect at 0, 4, 8, and 24 hours, while amoxicillin and the combination of amoxicillin and extract have a bacteriostatic effect at 4, 8, and 24 hours. Based on the results of the One Way ANOVA test with a 95% confidence level (p <0,05) showed a significant effect of kaffir lime leaf extract on the growth of Bacillus cereus.
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spelling doaj.art-9a0192814416432f8ba9b9e1d10f67b72023-04-26T23:44:08ZindFakultas Kesehatan Universitas Nahdlatul Ulama SurabayaMedical Technology and Public Health Journal2549-189X2549-29932023-04-017110.33086/mtphj.v7i1.3837Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHODchylen setiyo rini0universitas muhammadiyah sidoarjo Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease that attacks the intestines. This study aims to determine the kaffir lime leaf extract in inhibiting the growth of Bacillus cereus bacteria using the Time-Kill Test method. This study used the Minimum Inhibitory Concentration (MIC) and Time-Kill Test methods. The MIC results in kaffir lime leaf extract that can inhibit bacteria at 2 mg/mL, while the Time-Kill Test shows a death phase at 24 hours. Kaffir lime leaf extract has a bacteriostatic effect at 0, 4, 8, and 24 hours, while amoxicillin and the combination of amoxicillin and extract have a bacteriostatic effect at 4, 8, and 24 hours. Based on the results of the One Way ANOVA test with a 95% confidence level (p <0,05) showed a significant effect of kaffir lime leaf extract on the growth of Bacillus cereus. https://journal2.unusa.ac.id/index.php/MTPHJ/article/view/3837Bacillus cereusKaffir Lime Leaves (citrus hystrix)MICtime kill tets
spellingShingle chylen setiyo rini
Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
Medical Technology and Public Health Journal
Bacillus cereus
Kaffir Lime Leaves (citrus hystrix)
MIC
time kill tets
title Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
title_full Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
title_fullStr Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
title_full_unstemmed Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
title_short Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
title_sort antimicrobic on kaffir lime citrus hystrix leaves against bacillus cereus kill test method
topic Bacillus cereus
Kaffir Lime Leaves (citrus hystrix)
MIC
time kill tets
url https://journal2.unusa.ac.id/index.php/MTPHJ/article/view/3837
work_keys_str_mv AT chylensetiyorini antimicrobiconkaffirlimecitrushystrixleavesagainstbacilluscereuskilltestmethod