Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD
Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease tha...
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Format: | Article |
Language: | Indonesian |
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Fakultas Kesehatan Universitas Nahdlatul Ulama Surabaya
2023-04-01
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Series: | Medical Technology and Public Health Journal |
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Online Access: | https://journal2.unusa.ac.id/index.php/MTPHJ/article/view/3837 |
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author | chylen setiyo rini |
author_facet | chylen setiyo rini |
author_sort | chylen setiyo rini |
collection | DOAJ |
description |
Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease that attacks the intestines. This study aims to determine the kaffir lime leaf extract in inhibiting the growth of Bacillus cereus bacteria using the Time-Kill Test method. This study used the Minimum Inhibitory Concentration (MIC) and Time-Kill Test methods. The MIC results in kaffir lime leaf extract that can inhibit bacteria at 2 mg/mL, while the Time-Kill Test shows a death phase at 24 hours. Kaffir lime leaf extract has a bacteriostatic effect at 0, 4, 8, and 24 hours, while amoxicillin and the combination of amoxicillin and extract have a bacteriostatic effect at 4, 8, and 24 hours. Based on the results of the One Way ANOVA test with a 95% confidence level (p <0,05) showed a significant effect of kaffir lime leaf extract on the growth of Bacillus cereus.
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first_indexed | 2024-04-09T15:46:30Z |
format | Article |
id | doaj.art-9a0192814416432f8ba9b9e1d10f67b7 |
institution | Directory Open Access Journal |
issn | 2549-189X 2549-2993 |
language | Indonesian |
last_indexed | 2024-04-09T15:46:30Z |
publishDate | 2023-04-01 |
publisher | Fakultas Kesehatan Universitas Nahdlatul Ulama Surabaya |
record_format | Article |
series | Medical Technology and Public Health Journal |
spelling | doaj.art-9a0192814416432f8ba9b9e1d10f67b72023-04-26T23:44:08ZindFakultas Kesehatan Universitas Nahdlatul Ulama SurabayaMedical Technology and Public Health Journal2549-189X2549-29932023-04-017110.33086/mtphj.v7i1.3837Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHODchylen setiyo rini0universitas muhammadiyah sidoarjo Kaffir lime (Citrus hystrix) is a horticultural plant that is widely used as a natural flavor in various food and beverage products. The content in lime leaves is useful for health, including flavonoids, minerals, vitamin C, limonoids, and carotenoids. Bacillus cereus causes a diarrheal disease that attacks the intestines. This study aims to determine the kaffir lime leaf extract in inhibiting the growth of Bacillus cereus bacteria using the Time-Kill Test method. This study used the Minimum Inhibitory Concentration (MIC) and Time-Kill Test methods. The MIC results in kaffir lime leaf extract that can inhibit bacteria at 2 mg/mL, while the Time-Kill Test shows a death phase at 24 hours. Kaffir lime leaf extract has a bacteriostatic effect at 0, 4, 8, and 24 hours, while amoxicillin and the combination of amoxicillin and extract have a bacteriostatic effect at 4, 8, and 24 hours. Based on the results of the One Way ANOVA test with a 95% confidence level (p <0,05) showed a significant effect of kaffir lime leaf extract on the growth of Bacillus cereus. https://journal2.unusa.ac.id/index.php/MTPHJ/article/view/3837Bacillus cereusKaffir Lime Leaves (citrus hystrix)MICtime kill tets |
spellingShingle | chylen setiyo rini Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD Medical Technology and Public Health Journal Bacillus cereus Kaffir Lime Leaves (citrus hystrix) MIC time kill tets |
title | Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD |
title_full | Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD |
title_fullStr | Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD |
title_full_unstemmed | Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD |
title_short | Antimicrobic On KAFFIR LIME (Citrus hystrix) leaves against Bacillus cereus : KILL TEST METHOD |
title_sort | antimicrobic on kaffir lime citrus hystrix leaves against bacillus cereus kill test method |
topic | Bacillus cereus Kaffir Lime Leaves (citrus hystrix) MIC time kill tets |
url | https://journal2.unusa.ac.id/index.php/MTPHJ/article/view/3837 |
work_keys_str_mv | AT chylensetiyorini antimicrobiconkaffirlimecitrushystrixleavesagainstbacilluscereuskilltestmethod |