Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis
The leading indicator for successful outcomes in in-vitro fertilization (IVF) is the quality of gametes in oocytes and sperm. Thus, advanced research aims to highlight the parameter in assessing these qualities – DNA fragmentation in sperm and oocyte development capacity (ODC) via evaluation of micr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2024.1274376/full |
| _version_ | 1797269057099005952 |
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| author | Ahmad Mohd Faizal Marjanu Hikmah Elias Norazilah Mat Jin Muhammad Azrai Abu Saiful Effendi Syafruddin Ani Amelia Zainuddin Nao Suzuki Abdul Kadir Abdul Karim |
| author_facet | Ahmad Mohd Faizal Marjanu Hikmah Elias Norazilah Mat Jin Muhammad Azrai Abu Saiful Effendi Syafruddin Ani Amelia Zainuddin Nao Suzuki Abdul Kadir Abdul Karim |
| author_sort | Ahmad Mohd Faizal |
| collection | DOAJ |
| description | The leading indicator for successful outcomes in in-vitro fertilization (IVF) is the quality of gametes in oocytes and sperm. Thus, advanced research aims to highlight the parameter in assessing these qualities – DNA fragmentation in sperm and oocyte development capacity (ODC) via evaluation of microenvironments involving its maturation process. Regarding oocytes, most evidence reveals the role of cumulus cells as non-invasive methods in assessing their development competency, mainly via gene expression evaluation. Our review aims to consolidate the evidence of GDF-9 derivatives, the HAS2, GREM1, and PTGS2 gene expression in cumulus cells used as ODC markers in relevant publications and tailored to current IVF outcomes. In addition to that, we also added the bioinformatic analysis in our review to strengthen the evidence aiming for a better understanding of the pathways and cluster of the genes of interest - HAS2, GREM1, and PTGS2 in cumulus cell level. Otherwise, the current non-invasive method can be used in exploring various causes of infertility that may affect these gene expressions at the cumulus cell level. Nevertheless, this method can also be used in assessing the ODC in various cohorts of women or as an improvement of markers following targeted tools or procedures by evaluating the advancement of these gene expressions following the targeted intervention. |
| first_indexed | 2024-04-25T01:42:19Z |
| format | Article |
| id | doaj.art-9a06665d105945ebaaa44639f057484e |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-25T01:42:19Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-9a06665d105945ebaaa44639f057484e2024-03-08T04:28:06ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-03-011510.3389/fendo.2024.12743761274376Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysisAhmad Mohd Faizal0Marjanu Hikmah Elias1Norazilah Mat Jin2Muhammad Azrai Abu3Saiful Effendi Syafruddin4Ani Amelia Zainuddin5Nao Suzuki6Abdul Kadir Abdul Karim7Department of Obstetrics & Gynecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, MalaysiaFaculty of Medicine & Health Sciences, Universiti Sains Islam Malaysia, Nilai, Negeri Sembilan, MalaysiaDepartment of Obstetrics & Gynecology, Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, Sungai Buloh, Selangor, MalaysiaDepartment of Obstetrics & Gynecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, MalaysiaMedical Molecular Biology Institute, National University of Malaysia, Kuala Lumpur, MalaysiaDepartment of Obstetrics & Gynecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, MalaysiaDepartment of Obstetrics & Gynecology, St Marianna School of Medicine, Kawasaki, JapanDepartment of Obstetrics & Gynecology, Faculty of Medicine, National University of Malaysia, Kuala Lumpur, MalaysiaThe leading indicator for successful outcomes in in-vitro fertilization (IVF) is the quality of gametes in oocytes and sperm. Thus, advanced research aims to highlight the parameter in assessing these qualities – DNA fragmentation in sperm and oocyte development capacity (ODC) via evaluation of microenvironments involving its maturation process. Regarding oocytes, most evidence reveals the role of cumulus cells as non-invasive methods in assessing their development competency, mainly via gene expression evaluation. Our review aims to consolidate the evidence of GDF-9 derivatives, the HAS2, GREM1, and PTGS2 gene expression in cumulus cells used as ODC markers in relevant publications and tailored to current IVF outcomes. In addition to that, we also added the bioinformatic analysis in our review to strengthen the evidence aiming for a better understanding of the pathways and cluster of the genes of interest - HAS2, GREM1, and PTGS2 in cumulus cell level. Otherwise, the current non-invasive method can be used in exploring various causes of infertility that may affect these gene expressions at the cumulus cell level. Nevertheless, this method can also be used in assessing the ODC in various cohorts of women or as an improvement of markers following targeted tools or procedures by evaluating the advancement of these gene expressions following the targeted intervention.https://www.frontiersin.org/articles/10.3389/fendo.2024.1274376/fullHAS2PTGS2grem1cumulus cellsoocytes development competencygene expression |
| spellingShingle | Ahmad Mohd Faizal Marjanu Hikmah Elias Norazilah Mat Jin Muhammad Azrai Abu Saiful Effendi Syafruddin Ani Amelia Zainuddin Nao Suzuki Abdul Kadir Abdul Karim Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis Frontiers in Endocrinology HAS2 PTGS2 grem1 cumulus cells oocytes development competency gene expression |
| title | Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis |
| title_full | Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis |
| title_fullStr | Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis |
| title_full_unstemmed | Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis |
| title_short | Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis |
| title_sort | unravelling the role of has2 grem1 and ptgs2 gene expression in cumulus cells implications for human oocyte development competency a systematic review and integrated bioinformatic analysis |
| topic | HAS2 PTGS2 grem1 cumulus cells oocytes development competency gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2024.1274376/full |
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