Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat
Abstract Background Osteoarthritis (OA) is one of the most prevalent chronic joint diseases while the precise genetic mechanism remains elusive. In this study, we investigated the gene expression profile in OA by microarray analysis. Results Histopathological characteristics of OA cartilage were exa...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | Hereditas |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41065-017-0039-z |
| _version_ | 1819177157229805568 |
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| author | Qing Fan Zhu Liu Chao Shen Hai Li Jing Ding Fangchun Jin Lin Sha Ziming Zhang |
| author_facet | Qing Fan Zhu Liu Chao Shen Hai Li Jing Ding Fangchun Jin Lin Sha Ziming Zhang |
| author_sort | Qing Fan |
| collection | DOAJ |
| description | Abstract Background Osteoarthritis (OA) is one of the most prevalent chronic joint diseases while the precise genetic mechanism remains elusive. In this study, we investigated the gene expression profile in OA by microarray analysis. Results Histopathological characteristics of OA cartilage were examined using a rat model of leptin-induced OA. Gene expression profile of leptin-induced articular cartilage and healthy rat cartilage were compared using genome-wide microarray hybridization. A total of 1857 genes differentially expressed genes (1197 upregulated and 660 downregulated) were identified, some of which are known to be associated with leptin-induced OA phenotype. These included genes related to MMPs, inflammatory factors, growth factors, apoptotic genes and osteogenic genes. In addition, upregulated expressions of some new candidate genes, which have hitherto fore not been linked to OA (such as BCL2L11) were detected in leptin-induced OA cartilage, which suggests that these genes might be important for OA molecular mechanism. Conclusion Our findings suggest that pathogenesis of leptin-induced OA involves modulation of expression of multiple genes, although the underlying molecular mechanisms need to be studied further. Further investigation of leptin-induced gene expression changes is needed to gain new insights into the molecular mechanism of OA pathogenesis. |
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| format | Article |
| id | doaj.art-9a09d2ea922c4e19acb0b1135161f9ab |
| institution | Directory Open Access Journal |
| issn | 1601-5223 |
| language | English |
| last_indexed | 2024-12-22T21:22:11Z |
| publishDate | 2017-07-01 |
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| series | Hereditas |
| spelling | doaj.art-9a09d2ea922c4e19acb0b1135161f9ab2022-12-21T18:12:10ZengBMCHereditas1601-52232017-07-0115511910.1186/s41065-017-0039-zMicroarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in ratQing Fan0Zhu Liu1Chao Shen2Hai Li3Jing Ding4Fangchun Jin5Lin Sha6Ziming Zhang7Departments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineDepartments of Pediatric Orthopedics, Xin Hua Hospital Affiliated to Shanghai Jiaotong University, School of MedicineAbstract Background Osteoarthritis (OA) is one of the most prevalent chronic joint diseases while the precise genetic mechanism remains elusive. In this study, we investigated the gene expression profile in OA by microarray analysis. Results Histopathological characteristics of OA cartilage were examined using a rat model of leptin-induced OA. Gene expression profile of leptin-induced articular cartilage and healthy rat cartilage were compared using genome-wide microarray hybridization. A total of 1857 genes differentially expressed genes (1197 upregulated and 660 downregulated) were identified, some of which are known to be associated with leptin-induced OA phenotype. These included genes related to MMPs, inflammatory factors, growth factors, apoptotic genes and osteogenic genes. In addition, upregulated expressions of some new candidate genes, which have hitherto fore not been linked to OA (such as BCL2L11) were detected in leptin-induced OA cartilage, which suggests that these genes might be important for OA molecular mechanism. Conclusion Our findings suggest that pathogenesis of leptin-induced OA involves modulation of expression of multiple genes, although the underlying molecular mechanisms need to be studied further. Further investigation of leptin-induced gene expression changes is needed to gain new insights into the molecular mechanism of OA pathogenesis.http://link.springer.com/article/10.1186/s41065-017-0039-zOsteoarthritisLeptinRatsDEG |
| spellingShingle | Qing Fan Zhu Liu Chao Shen Hai Li Jing Ding Fangchun Jin Lin Sha Ziming Zhang Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat Hereditas Osteoarthritis Leptin Rats DEG |
| title | Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat |
| title_full | Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat |
| title_fullStr | Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat |
| title_full_unstemmed | Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat |
| title_short | Microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin-induced knee joint osteoarthritis in rat |
| title_sort | microarray study of gene expression profile to identify new candidate genes involved in the molecular mechanism of leptin induced knee joint osteoarthritis in rat |
| topic | Osteoarthritis Leptin Rats DEG |
| url | http://link.springer.com/article/10.1186/s41065-017-0039-z |
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