Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources
The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake...
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| Format: | Article |
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MDPI AG
2020-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/1/109 |
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| author | Manel Ben Abdallah Fatma Karray Sami Sayadi |
| author_facet | Manel Ben Abdallah Fatma Karray Sami Sayadi |
| author_sort | Manel Ben Abdallah |
| collection | DOAJ |
| description | The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to <i>Natrinema altunense</i> and <i>Haloterrigena jeotgali</i>, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield. |
| first_indexed | 2024-12-10T22:03:22Z |
| format | Article |
| id | doaj.art-9a0b8e734d25431c99dd44184afdc09d |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-10T22:03:22Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9a0b8e734d25431c99dd44184afdc09d2022-12-22T01:31:50ZengMDPI AGBiomolecules2218-273X2020-01-0110110910.3390/biom10010109biom10010109Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon SourcesManel Ben Abdallah0Fatma Karray1Sami Sayadi2Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, BP 1177, Sfax 3018, TunisiaLaboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, BP 1177, Sfax 3018, TunisiaCenter for Sustainable Development, College of Arts and Sciences, Qatar University, Doha 2713, QatarThe large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to <i>Natrinema altunense</i> and <i>Haloterrigena jeotgali</i>, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield.https://www.mdpi.com/2218-273X/10/1/109polyhydroxyalkanoatebiodegradable polymerhypersaline lakepha-producing archaeacarbon sources |
| spellingShingle | Manel Ben Abdallah Fatma Karray Sami Sayadi Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources Biomolecules polyhydroxyalkanoate biodegradable polymer hypersaline lake pha-producing archaea carbon sources |
| title | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_full | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_fullStr | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_full_unstemmed | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_short | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_sort | production of polyhydroxyalkanoates by two halophilic archaeal isolates from chott el jerid using inexpensive carbon sources |
| topic | polyhydroxyalkanoate biodegradable polymer hypersaline lake pha-producing archaea carbon sources |
| url | https://www.mdpi.com/2218-273X/10/1/109 |
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