Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems
Cellulose crystallinity is a crucial factor influencing stem strength and, consequently, wheat lodging. However, the genetic dissection of cellulose crystallinity is less reported due to the difficulty of its measurement. In this study, VIS/NIR spectra and cellulose crystallinity were measured for a...
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MDPI AG
2024-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/3028 |
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| author | Jianguo Li Peimin Zhao Liyan Zhao Qiang Chen Shikun Nong Qiang Li Lingqiang Wang |
| author_facet | Jianguo Li Peimin Zhao Liyan Zhao Qiang Chen Shikun Nong Qiang Li Lingqiang Wang |
| author_sort | Jianguo Li |
| collection | DOAJ |
| description | Cellulose crystallinity is a crucial factor influencing stem strength and, consequently, wheat lodging. However, the genetic dissection of cellulose crystallinity is less reported due to the difficulty of its measurement. In this study, VIS/NIR spectra and cellulose crystallinity were measured for a wheat accession panel with diverse genetic backgrounds. We developed a reliable VIS/NIR model for cellulose crystallinity with a high determination coefficient (R<sup>2</sup>) (0.95) and residual prediction deviation (RPD) (4.04), enabling the rapid screening of wheat samples. A GWAS of the cellulose crystallinity in 326 wheat accessions revealed 14 significant SNPs and 13 QTLs. Two candidate genes, <i>TraesCS4B03G0029800</i> and <i>TraesCS5B03G1085500,</i> were identified. In summary, this study establishes an efficient method for the measurement of cellulose crystallinity in wheat stems and provides a genetic basis for enhancing lodging resistance in wheat. |
| first_indexed | 2024-04-25T00:27:53Z |
| format | Article |
| id | doaj.art-9a0dea32e0154510b6a24ba5e8bb7b34 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:27:53Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9a0dea32e0154510b6a24ba5e8bb7b342024-03-12T16:47:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01255302810.3390/ijms25053028Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat StemsJianguo Li0Peimin Zhao1Liyan Zhao2Qiang Chen3Shikun Nong4Qiang Li5Lingqiang Wang6College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation & Utilization of Subtropical Agro-Bioresources, College of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCellulose crystallinity is a crucial factor influencing stem strength and, consequently, wheat lodging. However, the genetic dissection of cellulose crystallinity is less reported due to the difficulty of its measurement. In this study, VIS/NIR spectra and cellulose crystallinity were measured for a wheat accession panel with diverse genetic backgrounds. We developed a reliable VIS/NIR model for cellulose crystallinity with a high determination coefficient (R<sup>2</sup>) (0.95) and residual prediction deviation (RPD) (4.04), enabling the rapid screening of wheat samples. A GWAS of the cellulose crystallinity in 326 wheat accessions revealed 14 significant SNPs and 13 QTLs. Two candidate genes, <i>TraesCS4B03G0029800</i> and <i>TraesCS5B03G1085500,</i> were identified. In summary, this study establishes an efficient method for the measurement of cellulose crystallinity in wheat stems and provides a genetic basis for enhancing lodging resistance in wheat.https://www.mdpi.com/1422-0067/25/5/3028cellulose crystallinitywheatVIS/NIRGWAS |
| spellingShingle | Jianguo Li Peimin Zhao Liyan Zhao Qiang Chen Shikun Nong Qiang Li Lingqiang Wang Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems International Journal of Molecular Sciences cellulose crystallinity wheat VIS/NIR GWAS |
| title | Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems |
| title_full | Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems |
| title_fullStr | Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems |
| title_full_unstemmed | Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems |
| title_short | Integrated VIS/NIR Spectrum and Genome-Wide Association Study for Genetic Dissection of Cellulose Crystallinity in Wheat Stems |
| title_sort | integrated vis nir spectrum and genome wide association study for genetic dissection of cellulose crystallinity in wheat stems |
| topic | cellulose crystallinity wheat VIS/NIR GWAS |
| url | https://www.mdpi.com/1422-0067/25/5/3028 |
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