Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation
Bromodomain containing (BRD) proteins play an essential role in many cellular processes. The aim of this study was to estimate activity of bromodomains during alga <i>Chara vulgaris</i> spermatids differentiation. The effect of a bromodomain inhibitor, JQ1 (100 μM), on the distribution o...
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MDPI AG
2020-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/9/6/1352 |
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| author | Agnieszka Wojtczak |
| author_facet | Agnieszka Wojtczak |
| author_sort | Agnieszka Wojtczak |
| collection | DOAJ |
| description | Bromodomain containing (BRD) proteins play an essential role in many cellular processes. The aim of this study was to estimate activity of bromodomains during alga <i>Chara vulgaris</i> spermatids differentiation. The effect of a bromodomain inhibitor, JQ1 (100 μM), on the distribution of individual stages of spermatids and their ultrastructure was studied. The material was Feulgen stained and analysed in an electron microscope. JQ1 caused shortening of the early stages of spermiogenesis and a reverse reaction at the later stages. Additionally, in the same antheridium, spermatids at distant developmental stages were present. On the ultrastructural level, chromatin fibril system disorders and significantly distended endoplasmic reticulum (ER) cisternae already at the early stages were observed. Many autolytic vacuoles were also visible. The ultrastructural disturbances intensified after prolonged treatment with JQ1. The obtained data show that JQ1 treatment led to changes in the spermatid number and disturbances in chromatin condensation and to cytoplasm reduction. The current studies show some similarities between <i>C. vulgaris</i> and mammals spermiogenesis. Taken together, these results suggest that JQ1 interferes with the spermatid differentiation on many interdependent levels and seems to induce ER stress, which leads to spermatid degeneration. Studies on the role of bromodomains in algae spermiogenesis have not been conducted so far. |
| first_indexed | 2024-03-10T19:29:51Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T19:29:51Z |
| publishDate | 2020-05-01 |
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| spelling | doaj.art-9a10704a2054457daccc47942ebacb022023-11-20T02:12:52ZengMDPI AGCells2073-44092020-05-0196135210.3390/cells9061352Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids DifferentiationAgnieszka Wojtczak0Institute of Experimental Biology, Department of Cytophysiology, University of Lodz, Faculty of Biology and Environmental Protection, 141/143 Pomorska, 90-236 Lodz, PolandBromodomain containing (BRD) proteins play an essential role in many cellular processes. The aim of this study was to estimate activity of bromodomains during alga <i>Chara vulgaris</i> spermatids differentiation. The effect of a bromodomain inhibitor, JQ1 (100 μM), on the distribution of individual stages of spermatids and their ultrastructure was studied. The material was Feulgen stained and analysed in an electron microscope. JQ1 caused shortening of the early stages of spermiogenesis and a reverse reaction at the later stages. Additionally, in the same antheridium, spermatids at distant developmental stages were present. On the ultrastructural level, chromatin fibril system disorders and significantly distended endoplasmic reticulum (ER) cisternae already at the early stages were observed. Many autolytic vacuoles were also visible. The ultrastructural disturbances intensified after prolonged treatment with JQ1. The obtained data show that JQ1 treatment led to changes in the spermatid number and disturbances in chromatin condensation and to cytoplasm reduction. The current studies show some similarities between <i>C. vulgaris</i> and mammals spermiogenesis. Taken together, these results suggest that JQ1 interferes with the spermatid differentiation on many interdependent levels and seems to induce ER stress, which leads to spermatid degeneration. Studies on the role of bromodomains in algae spermiogenesis have not been conducted so far.https://www.mdpi.com/2073-4409/9/6/1352bromodomain<i>Chara vulgaris</i>chromatinchromatin remodeling complexelectron microscopyendoplasmic reticulum stress |
| spellingShingle | Agnieszka Wojtczak Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation Cells bromodomain <i>Chara vulgaris</i> chromatin chromatin remodeling complex electron microscopy endoplasmic reticulum stress |
| title | Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation |
| title_full | Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation |
| title_fullStr | Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation |
| title_full_unstemmed | Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation |
| title_short | Blocking the Bromodomains Function Contributes to Disturbances in Alga <i>Chara vulgaris</i> Spermatids Differentiation |
| title_sort | blocking the bromodomains function contributes to disturbances in alga i chara vulgaris i spermatids differentiation |
| topic | bromodomain <i>Chara vulgaris</i> chromatin chromatin remodeling complex electron microscopy endoplasmic reticulum stress |
| url | https://www.mdpi.com/2073-4409/9/6/1352 |
| work_keys_str_mv | AT agnieszkawojtczak blockingthebromodomainsfunctioncontributestodisturbancesinalgaicharavulgarisispermatidsdifferentiation |