Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21
The objective of this study is to evaluate the effects of a strictly essential amino acid (lysine or threonine; EAA) deficiency on energy metabolism in growing rats. Rats were fed for three weeks severely (15% and 25% of recommendation), moderately (40% and 60%), and adequate (75% and 100%) lysine o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/1/197 |
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| author | Joanna Moro Gaëtan Roisné-Hamelin Catherine Chaumontet Patrick C. Even Anne Blais Celine Cansell Julien Piedcoq Claire Gaudichon Daniel Tomé Dalila Azzout-Marniche |
| author_facet | Joanna Moro Gaëtan Roisné-Hamelin Catherine Chaumontet Patrick C. Even Anne Blais Celine Cansell Julien Piedcoq Claire Gaudichon Daniel Tomé Dalila Azzout-Marniche |
| author_sort | Joanna Moro |
| collection | DOAJ |
| description | The objective of this study is to evaluate the effects of a strictly essential amino acid (lysine or threonine; EAA) deficiency on energy metabolism in growing rats. Rats were fed for three weeks severely (15% and 25% of recommendation), moderately (40% and 60%), and adequate (75% and 100%) lysine or threonine-deficient diets. Food intake and body weight were measured daily and indirect calorimetry was performed the week three. At the end of the experimentation, body composition, gene expression, and biochemical analysis were performed. Lysine and threonine deficiency induced a lower body weight gain and an increase in relative food intake. Lysine or threonine deficiency induced liver FGF21 synthesis and plasma release. However, no changes in energy expenditure were observed for lysine deficiency, unlike threonine deficiency, which leads to a decrease in total and resting energy expenditure. Interestingly, threonine severe deficiency, but not lysine deficiency, increase orexigenic and decreases anorexigenic hypothalamic neuropeptides expression, which could explain the higher food intake. Our results show that the deficiency in one EAA, induces a decrease in body weight gain, despite an increased relative food intake, without any increase in energy expenditure despite an induction of FGF21. |
| first_indexed | 2024-03-09T09:43:47Z |
| format | Article |
| id | doaj.art-9a12531249ea48ea8a05ec42b5913414 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T09:43:47Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-9a12531249ea48ea8a05ec42b59134142023-12-02T00:46:28ZengMDPI AGNutrients2072-66432022-12-0115119710.3390/nu15010197Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21Joanna Moro0Gaëtan Roisné-Hamelin1Catherine Chaumontet2Patrick C. Even3Anne Blais4Celine Cansell5Julien Piedcoq6Claire Gaudichon7Daniel Tomé8Dalila Azzout-Marniche9AgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceAgroParisTech, UMR PNCA, Institut National de la Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Saclay, 91120 Palaiseau, FranceThe objective of this study is to evaluate the effects of a strictly essential amino acid (lysine or threonine; EAA) deficiency on energy metabolism in growing rats. Rats were fed for three weeks severely (15% and 25% of recommendation), moderately (40% and 60%), and adequate (75% and 100%) lysine or threonine-deficient diets. Food intake and body weight were measured daily and indirect calorimetry was performed the week three. At the end of the experimentation, body composition, gene expression, and biochemical analysis were performed. Lysine and threonine deficiency induced a lower body weight gain and an increase in relative food intake. Lysine or threonine deficiency induced liver FGF21 synthesis and plasma release. However, no changes in energy expenditure were observed for lysine deficiency, unlike threonine deficiency, which leads to a decrease in total and resting energy expenditure. Interestingly, threonine severe deficiency, but not lysine deficiency, increase orexigenic and decreases anorexigenic hypothalamic neuropeptides expression, which could explain the higher food intake. Our results show that the deficiency in one EAA, induces a decrease in body weight gain, despite an increased relative food intake, without any increase in energy expenditure despite an induction of FGF21.https://www.mdpi.com/2072-6643/15/1/197lysine deficiencythreonine deficiencyenergy metabolismFGF21energy expenditurefood intake |
| spellingShingle | Joanna Moro Gaëtan Roisné-Hamelin Catherine Chaumontet Patrick C. Even Anne Blais Celine Cansell Julien Piedcoq Claire Gaudichon Daniel Tomé Dalila Azzout-Marniche Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21 Nutrients lysine deficiency threonine deficiency energy metabolism FGF21 energy expenditure food intake |
| title | Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21 |
| title_full | Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21 |
| title_fullStr | Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21 |
| title_full_unstemmed | Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21 |
| title_short | Lysine or Threonine Deficiency Decreases Body Weight Gain in Growing Rats despite an Increase in Food Intake without Increasing Energy Expenditure in Response to FGF21 |
| title_sort | lysine or threonine deficiency decreases body weight gain in growing rats despite an increase in food intake without increasing energy expenditure in response to fgf21 |
| topic | lysine deficiency threonine deficiency energy metabolism FGF21 energy expenditure food intake |
| url | https://www.mdpi.com/2072-6643/15/1/197 |
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