Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b
Summary: SARS-CoV-2, the causative agent of the present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated in facilitating infection and pathogenesis through their interaction with cellular components. Among these proteins, accessory protein O...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004222017163 |
| _version_ | 1798023064254939136 |
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| author | Tránsito García-García Raúl Fernández-Rodríguez Natalia Redondo Ana de Lucas-Rius Sara Zaldívar-López Blanca Dies López-Ayllón José M. Suárez-Cárdenas Ángeles Jiménez-Marín María Montoya Juan J. Garrido |
| author_facet | Tránsito García-García Raúl Fernández-Rodríguez Natalia Redondo Ana de Lucas-Rius Sara Zaldívar-López Blanca Dies López-Ayllón José M. Suárez-Cárdenas Ángeles Jiménez-Marín María Montoya Juan J. Garrido |
| author_sort | Tránsito García-García |
| collection | DOAJ |
| description | Summary: SARS-CoV-2, the causative agent of the present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated in facilitating infection and pathogenesis through their interaction with cellular components. Among these proteins, accessory protein ORF7a and ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7a and ORF7b, respectively, to explore more in depth the role of each accessory protein in the pathological manifestation leading to COVID-19. Bioinformatic analysis and integration of transcriptome results identified defined canonical pathways and functional groupings revealing that after expression of ORF7a or ORF7b, the lung cells are potentially altered to create conditions more favorable for SARS-CoV-2, by inhibiting the IFN-I response, increasing proinflammatory cytokines release, and altering cell metabolic activity and adhesion. Based on these results, it is plausible to suggest that ORF7a or ORF7b could be used as biomarkers of progression in this pandemic. |
| first_indexed | 2024-04-11T17:40:05Z |
| format | Article |
| id | doaj.art-9a13bebe61cf4759bf74166f3e5e5a67 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-11T17:40:05Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-9a13bebe61cf4759bf74166f3e5e5a672022-12-22T04:11:31ZengElsevieriScience2589-00422022-11-012511105444Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7bTránsito García-García0Raúl Fernández-Rodríguez1Natalia Redondo2Ana de Lucas-Rius3Sara Zaldívar-López4Blanca Dies López-Ayllón5José M. Suárez-Cárdenas6Ángeles Jiménez-Marín7María Montoya8Juan J. Garrido9Immunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, Department of Genetics, University of Córdoba, Córdoba, Spain; Maimónides Biomedical Research Institute of Córdoba (IMIBIC), GA-14 Research Group, Córdoba, SpainImmunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, Department of Genetics, University of Córdoba, Córdoba, Spain; Maimónides Biomedical Research Institute of Córdoba (IMIBIC), GA-14 Research Group, Córdoba, SpainMolecular Biomedicine Department, Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, Madrid 28040, SpainMolecular Biomedicine Department, Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, Madrid 28040, SpainImmunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, Department of Genetics, University of Córdoba, Córdoba, Spain; Maimónides Biomedical Research Institute of Córdoba (IMIBIC), GA-14 Research Group, Córdoba, SpainMolecular Biomedicine Department, Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, Madrid 28040, SpainImmunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, Department of Genetics, University of Córdoba, Córdoba, Spain; Maimónides Biomedical Research Institute of Córdoba (IMIBIC), GA-14 Research Group, Córdoba, SpainImmunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, Department of Genetics, University of Córdoba, Córdoba, Spain; Maimónides Biomedical Research Institute of Córdoba (IMIBIC), GA-14 Research Group, Córdoba, SpainMolecular Biomedicine Department, Centro de Investigaciones Biológicas Margarita Salas (CIB), CSIC, Madrid 28040, Spain; Corresponding authorImmunogenomics and Molecular Pathogenesis Group, UIC Zoonoses and Emergent Diseases ENZOEM, Department of Genetics, University of Córdoba, Córdoba, Spain; Maimónides Biomedical Research Institute of Córdoba (IMIBIC), GA-14 Research Group, Córdoba, Spain; Corresponding authorSummary: SARS-CoV-2, the causative agent of the present COVID-19 pandemic, possesses eleven accessory proteins encoded in its genome, and some have been implicated in facilitating infection and pathogenesis through their interaction with cellular components. Among these proteins, accessory protein ORF7a and ORF7b functions are poorly understood. In this study, A549 cells were transduced to express ORF7a and ORF7b, respectively, to explore more in depth the role of each accessory protein in the pathological manifestation leading to COVID-19. Bioinformatic analysis and integration of transcriptome results identified defined canonical pathways and functional groupings revealing that after expression of ORF7a or ORF7b, the lung cells are potentially altered to create conditions more favorable for SARS-CoV-2, by inhibiting the IFN-I response, increasing proinflammatory cytokines release, and altering cell metabolic activity and adhesion. Based on these results, it is plausible to suggest that ORF7a or ORF7b could be used as biomarkers of progression in this pandemic.http://www.sciencedirect.com/science/article/pii/S2589004222017163ImmunologyImmune responseCell biologyTranscriptomics |
| spellingShingle | Tránsito García-García Raúl Fernández-Rodríguez Natalia Redondo Ana de Lucas-Rius Sara Zaldívar-López Blanca Dies López-Ayllón José M. Suárez-Cárdenas Ángeles Jiménez-Marín María Montoya Juan J. Garrido Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b iScience Immunology Immune response Cell biology Transcriptomics |
| title | Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b |
| title_full | Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b |
| title_fullStr | Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b |
| title_full_unstemmed | Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b |
| title_short | Impairment of antiviral immune response and disruption of cellular functions by SARS-CoV-2 ORF7a and ORF7b |
| title_sort | impairment of antiviral immune response and disruption of cellular functions by sars cov 2 orf7a and orf7b |
| topic | Immunology Immune response Cell biology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004222017163 |
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