Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway
<i>Artemisia argyi</i> Levl. et Van (<i>A. argyi</i>) is a traditional medicinal plant, which is widely used in health, food and medicine. Thujone is an important cyclic monoterpene derivative in the volatile oil of <i>A. argyi</i> leaves with multiple efficacy. A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/2/232 |
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| author | Tingting Zhao Changjie Chen Jinxin Li Dandan Luo Yuhuan Miao Chun Gui Qi Liu Dahui Liu |
| author_facet | Tingting Zhao Changjie Chen Jinxin Li Dandan Luo Yuhuan Miao Chun Gui Qi Liu Dahui Liu |
| author_sort | Tingting Zhao |
| collection | DOAJ |
| description | <i>Artemisia argyi</i> Levl. et Van (<i>A. argyi</i>) is a traditional medicinal plant, which is widely used in health, food and medicine. Thujone is an important cyclic monoterpene derivative in the volatile oil of <i>A. argyi</i> leaves with multiple efficacy. Although the thujone synthetic pathway has been preliminarily analyzed in very few species, genes related to the thujone content in <i>A. argyi</i> leaves remain largely unknown. In this study, we identify candidate genes involved in the synthesis and regulation of thujone content in <i>A. argyi</i> leaves by the comparative transcriptome analysis of two group materials with high and low thujone content. A total of 89 candidate genes related to thujone content are identified including one gene involved in the mevalonate pathway, three genes involved in the methylerythritol phosphate pathway, 19 genes involved in the metabolic process from geranyl pyrophosphate to thujone (four b-terpene synthase, five cytochrome P450, five dehydrogenase, and five reductase-encoding genes) and 66 transcription factor-encoding genes. Taken together, our results provide valuable gene resources for further analyzing the synthetic and regulatory pathway of thujone in <i>A. argyi</i>. |
| first_indexed | 2024-03-11T08:45:08Z |
| format | Article |
| id | doaj.art-9a1b7d80b5b647ec97c77cf0b8df7cd2 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T08:45:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-9a1b7d80b5b647ec97c77cf0b8df7cd22023-11-16T20:50:26ZengMDPI AGHorticulturae2311-75242023-02-019223210.3390/horticulturae9020232Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory PathwayTingting Zhao0Changjie Chen1Jinxin Li2Dandan Luo3Yuhuan Miao4Chun Gui5Qi Liu6Dahui Liu7Key Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, ChinaKey Laboratory of Traditional Chinese Medicine Resources and Chemistry of Hubei Province, Hubei University of Chinese Medicine, Wuhan 430065, China<i>Artemisia argyi</i> Levl. et Van (<i>A. argyi</i>) is a traditional medicinal plant, which is widely used in health, food and medicine. Thujone is an important cyclic monoterpene derivative in the volatile oil of <i>A. argyi</i> leaves with multiple efficacy. Although the thujone synthetic pathway has been preliminarily analyzed in very few species, genes related to the thujone content in <i>A. argyi</i> leaves remain largely unknown. In this study, we identify candidate genes involved in the synthesis and regulation of thujone content in <i>A. argyi</i> leaves by the comparative transcriptome analysis of two group materials with high and low thujone content. A total of 89 candidate genes related to thujone content are identified including one gene involved in the mevalonate pathway, three genes involved in the methylerythritol phosphate pathway, 19 genes involved in the metabolic process from geranyl pyrophosphate to thujone (four b-terpene synthase, five cytochrome P450, five dehydrogenase, and five reductase-encoding genes) and 66 transcription factor-encoding genes. Taken together, our results provide valuable gene resources for further analyzing the synthetic and regulatory pathway of thujone in <i>A. argyi</i>.https://www.mdpi.com/2311-7524/9/2/232<i>Artemisia argyi</i>thujoneMVAMEPterpene synthase |
| spellingShingle | Tingting Zhao Changjie Chen Jinxin Li Dandan Luo Yuhuan Miao Chun Gui Qi Liu Dahui Liu Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway Horticulturae <i>Artemisia argyi</i> thujone MVA MEP terpene synthase |
| title | Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway |
| title_full | Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway |
| title_fullStr | Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway |
| title_full_unstemmed | Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway |
| title_short | Comparative Transcriptome Analysis of High and Low Thujone-Producing <i>Artemisia argyi</i> Reveals Candidate Genes for Thujone Synthetic and Regulatory Pathway |
| title_sort | comparative transcriptome analysis of high and low thujone producing i artemisia argyi i reveals candidate genes for thujone synthetic and regulatory pathway |
| topic | <i>Artemisia argyi</i> thujone MVA MEP terpene synthase |
| url | https://www.mdpi.com/2311-7524/9/2/232 |
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