Consortium of Probiotics Attenuates Colonization of Clostridioides difficile
Clostridioides difficile infection (CDI) is increasing morbidity and mortality rates globally. Fecal microbiota transplantation (FMT), an effective therapy for eliminating Clostridioides difficile (C. difficile), cannot be used extensive due to a range of challenges. Probiotics thus constitutes a pr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02871/full |
| _version_ | 1811325596637069312 |
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| author | Xianping Li Xianping Li Qiongfang Chu Qiongfang Chu Qiongfang Chu Yuanming Huang Yuanming Huang Yuchun Xiao Yuchun Xiao Liqiong Song Liqiong Song Siyi Zhu Siyi Zhu Ying Kang Ying Kang Shan Lu Shan Lu Jianguo Xu Jianguo Xu Zhihong Ren Zhihong Ren |
| author_facet | Xianping Li Xianping Li Qiongfang Chu Qiongfang Chu Qiongfang Chu Yuanming Huang Yuanming Huang Yuchun Xiao Yuchun Xiao Liqiong Song Liqiong Song Siyi Zhu Siyi Zhu Ying Kang Ying Kang Shan Lu Shan Lu Jianguo Xu Jianguo Xu Zhihong Ren Zhihong Ren |
| author_sort | Xianping Li |
| collection | DOAJ |
| description | Clostridioides difficile infection (CDI) is increasing morbidity and mortality rates globally. Fecal microbiota transplantation (FMT), an effective therapy for eliminating Clostridioides difficile (C. difficile), cannot be used extensive due to a range of challenges. Probiotics thus constitutes a promising alternative therapy. In our study, we evaluated the effect of consortium of probiotics including five Lactobacilli strains and two Bifidobacterium strains on the colonization of toxigenic BI/NAP1/027 C. difficile in a mouse model. The results of 16S rRNA sequencing and targeted metabolomics showed the consortium of probiotics effectively decreased the colonization of C. difficile, changed the α- and β-diversity of the gut microbiota, decreased the primary bile acids, and increased the secondary bile acids. Spearman’s correlation showed that some of the OTUs such as Akkermansia, Bacteroides, Blautia et al. were positively correlated with C. difficile numbers and the primary bile acids, and negatively correlated with the secondary bile acids. However, some of the OTUs, such as Butyricicoccus, Ruminococcus, and Rikenellaceae, were negatively correlated with C. difficile copies and the primary bile acids, and positively correlated with the secondary bile acids. In summary, the consortium of probiotics effectively decreases the colonization of C. difficile, probably via alteration of gut microbiota and bile acids. Our probiotics mixture thus offers a promising FMT alternative. |
| first_indexed | 2024-04-13T14:37:10Z |
| format | Article |
| id | doaj.art-9a213c39d3ab4f56a7b7a37e3c269e5a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T14:37:10Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-9a213c39d3ab4f56a7b7a37e3c269e5a2022-12-22T02:43:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-12-011010.3389/fmicb.2019.02871476703Consortium of Probiotics Attenuates Colonization of Clostridioides difficileXianping Li0Xianping Li1Qiongfang Chu2Qiongfang Chu3Qiongfang Chu4Yuanming Huang5Yuanming Huang6Yuchun Xiao7Yuchun Xiao8Liqiong Song9Liqiong Song10Siyi Zhu11Siyi Zhu12Ying Kang13Ying Kang14Shan Lu15Shan Lu16Jianguo Xu17Jianguo Xu18Zhihong Ren19Zhihong Ren20State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaBeijing Dongcheng District Longtan Community Health Center, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, ChinaResearch Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaClostridioides difficile infection (CDI) is increasing morbidity and mortality rates globally. Fecal microbiota transplantation (FMT), an effective therapy for eliminating Clostridioides difficile (C. difficile), cannot be used extensive due to a range of challenges. Probiotics thus constitutes a promising alternative therapy. In our study, we evaluated the effect of consortium of probiotics including five Lactobacilli strains and two Bifidobacterium strains on the colonization of toxigenic BI/NAP1/027 C. difficile in a mouse model. The results of 16S rRNA sequencing and targeted metabolomics showed the consortium of probiotics effectively decreased the colonization of C. difficile, changed the α- and β-diversity of the gut microbiota, decreased the primary bile acids, and increased the secondary bile acids. Spearman’s correlation showed that some of the OTUs such as Akkermansia, Bacteroides, Blautia et al. were positively correlated with C. difficile numbers and the primary bile acids, and negatively correlated with the secondary bile acids. However, some of the OTUs, such as Butyricicoccus, Ruminococcus, and Rikenellaceae, were negatively correlated with C. difficile copies and the primary bile acids, and positively correlated with the secondary bile acids. In summary, the consortium of probiotics effectively decreases the colonization of C. difficile, probably via alteration of gut microbiota and bile acids. Our probiotics mixture thus offers a promising FMT alternative.https://www.frontiersin.org/article/10.3389/fmicb.2019.02871/fullC. difficileprobioticsLactobacillusBifidobacteriumbile acidsshort-chain fatty acids |
| spellingShingle | Xianping Li Xianping Li Qiongfang Chu Qiongfang Chu Qiongfang Chu Yuanming Huang Yuanming Huang Yuchun Xiao Yuchun Xiao Liqiong Song Liqiong Song Siyi Zhu Siyi Zhu Ying Kang Ying Kang Shan Lu Shan Lu Jianguo Xu Jianguo Xu Zhihong Ren Zhihong Ren Consortium of Probiotics Attenuates Colonization of Clostridioides difficile Frontiers in Microbiology C. difficile probiotics Lactobacillus Bifidobacterium bile acids short-chain fatty acids |
| title | Consortium of Probiotics Attenuates Colonization of Clostridioides difficile |
| title_full | Consortium of Probiotics Attenuates Colonization of Clostridioides difficile |
| title_fullStr | Consortium of Probiotics Attenuates Colonization of Clostridioides difficile |
| title_full_unstemmed | Consortium of Probiotics Attenuates Colonization of Clostridioides difficile |
| title_short | Consortium of Probiotics Attenuates Colonization of Clostridioides difficile |
| title_sort | consortium of probiotics attenuates colonization of clostridioides difficile |
| topic | C. difficile probiotics Lactobacillus Bifidobacterium bile acids short-chain fatty acids |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02871/full |
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