In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient
The beauty industry is actively searching for solutions to prevent skin aging. Some of the crucial elements protecting cells from the aging process are telomere shortening, telomerase expression, cell senescence, and homeostasis of the redox system. Modification of these factors using natural antiox...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/22/8101 |
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| author | José Quiles Maria Cabrera Jonathan Jones Menelaos Tsapekos Nuria Caturla |
| author_facet | José Quiles Maria Cabrera Jonathan Jones Menelaos Tsapekos Nuria Caturla |
| author_sort | José Quiles |
| collection | DOAJ |
| description | The beauty industry is actively searching for solutions to prevent skin aging. Some of the crucial elements protecting cells from the aging process are telomere shortening, telomerase expression, cell senescence, and homeostasis of the redox system. Modification of these factors using natural antioxidants is an appealing way to support healthy skin aging. Therefore, in this study, we sought to investigate the antiaging efficacy of a specific combination of four botanical extracts (pomegranate, sweet orange, Cistanche and <i>Centella asiatica</i>) with proven antioxidant properties. To this end, normal human dermal fibroblasts were used as a cell model and the following studies were performed: cell proliferation was established by means of the MTT assay and the intracellular ROS levels in stress-induced premature senescence fibroblasts; telomere length measurement was performed under standard cell culture conditions using qPCR and under oxidative stress conditions using a variation of the Q-FISH technique; telomerase activity was examined by means of Q-TRAP; and AGE quantification was completed by means of ELISA assay in UV-irradiated fibroblasts. As a result, the botanical blend significantly reversed the H<sub>2</sub>O<sub>2</sub>-induced decrease in cell viability and reduced H<sub>2</sub>O<sub>2</sub>-induced ROS. Additionally, the presence of the botanical ingredient reduced the telomere shortening rate in both stressed and non-stressed replicating fibroblasts, and under oxidative stress conditions, the fibroblasts presented a higher median and 20th percentile telomere length, as well as a lower percentage of short telomeres (<3 Kbp) compared with untreated fibroblasts. Furthermore, the ingredient transiently increased relative telomerase activity after 24 h and prevented the accumulation of UVR-induced glycated species. The results support the potential use of this four-component plant-based ingredient as an antiaging agent. |
| first_indexed | 2024-03-09T18:07:08Z |
| format | Article |
| id | doaj.art-9a21e2bf182141b7881789bb6eacd141 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:07:08Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9a21e2bf182141b7881789bb6eacd1412023-11-24T09:26:30ZengMDPI AGMolecules1420-30492022-11-012722810110.3390/molecules27228101In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based IngredientJosé Quiles0Maria Cabrera1Jonathan Jones2Menelaos Tsapekos3Nuria Caturla4Research Projects Department, Life Length SL, Calle Miguel Angel 11, 28010 Madrid, SpainResearch and Development Department, Monteloeder SL, Miguel Servet 16, 03203 Elche, SpainResearch and Development Department, Monteloeder SL, Miguel Servet 16, 03203 Elche, SpainResearch Projects Department, Life Length SL, Calle Miguel Angel 11, 28010 Madrid, SpainResearch and Development Department, Monteloeder SL, Miguel Servet 16, 03203 Elche, SpainThe beauty industry is actively searching for solutions to prevent skin aging. Some of the crucial elements protecting cells from the aging process are telomere shortening, telomerase expression, cell senescence, and homeostasis of the redox system. Modification of these factors using natural antioxidants is an appealing way to support healthy skin aging. Therefore, in this study, we sought to investigate the antiaging efficacy of a specific combination of four botanical extracts (pomegranate, sweet orange, Cistanche and <i>Centella asiatica</i>) with proven antioxidant properties. To this end, normal human dermal fibroblasts were used as a cell model and the following studies were performed: cell proliferation was established by means of the MTT assay and the intracellular ROS levels in stress-induced premature senescence fibroblasts; telomere length measurement was performed under standard cell culture conditions using qPCR and under oxidative stress conditions using a variation of the Q-FISH technique; telomerase activity was examined by means of Q-TRAP; and AGE quantification was completed by means of ELISA assay in UV-irradiated fibroblasts. As a result, the botanical blend significantly reversed the H<sub>2</sub>O<sub>2</sub>-induced decrease in cell viability and reduced H<sub>2</sub>O<sub>2</sub>-induced ROS. Additionally, the presence of the botanical ingredient reduced the telomere shortening rate in both stressed and non-stressed replicating fibroblasts, and under oxidative stress conditions, the fibroblasts presented a higher median and 20th percentile telomere length, as well as a lower percentage of short telomeres (<3 Kbp) compared with untreated fibroblasts. Furthermore, the ingredient transiently increased relative telomerase activity after 24 h and prevented the accumulation of UVR-induced glycated species. The results support the potential use of this four-component plant-based ingredient as an antiaging agent.https://www.mdpi.com/1420-3049/27/22/8101plant extractsantiagingtelomere lengthantioxidant agentstelomeraseantiglycation |
| spellingShingle | José Quiles Maria Cabrera Jonathan Jones Menelaos Tsapekos Nuria Caturla In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient Molecules plant extracts antiaging telomere length antioxidant agents telomerase antiglycation |
| title | In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient |
| title_full | In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient |
| title_fullStr | In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient |
| title_full_unstemmed | In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient |
| title_short | In Vitro Determination of the Skin Anti-Aging Potential of Four-Component Plant-Based Ingredient |
| title_sort | in vitro determination of the skin anti aging potential of four component plant based ingredient |
| topic | plant extracts antiaging telomere length antioxidant agents telomerase antiglycation |
| url | https://www.mdpi.com/1420-3049/27/22/8101 |
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