Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar
Plants have evolved a sophisticated system to respond to various stresses. Fungal attack or infection is one of the most important biotic stresses for most plants. During the defense response to fungal infection, the plant hormones jasmonic acid (JA) and salicylic acid (SA) play critical roles. Here...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Biomolecules |
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| Online Access: | http://www.mdpi.com/2218-273X/9/1/12 |
| _version_ | 1818024092337438720 |
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| author | Jie Luo Wenxiu Xia Pei Cao Zheng’ang Xiao Yan Zhang Meifeng Liu Chang Zhan Nian Wang |
| author_facet | Jie Luo Wenxiu Xia Pei Cao Zheng’ang Xiao Yan Zhang Meifeng Liu Chang Zhan Nian Wang |
| author_sort | Jie Luo |
| collection | DOAJ |
| description | Plants have evolved a sophisticated system to respond to various stresses. Fungal attack or infection is one of the most important biotic stresses for most plants. During the defense response to fungal infection, the plant hormones jasmonic acid (JA) and salicylic acid (SA) play critical roles. Here, gene expression data on JA/SA treatments and Melampsora larici-populina (MLP) infection were generated. Integrated transcriptome analyses of these data were performed, and 943 genes in total were identified as common responsive genes (CRG). Gene ontology (GO) term analysis revealed that the genes from CRG are generally involved in the processes of stress responses, metabolism, and growth and development. The further cluster analysis of the CRG identified a set of core genes that are involved in the JA/SA-mediated response to fungal defense with distinct gene expression profiles upon JA/SA treatment, which highlighted the different effects of these two hormones on plant fungal defenses. The modifications of several pathways relative to metabolism, biotic stress, and plant hormone signal pathways suggest the possible roles of JA/SA on the regulation of growth and defense responses. Co-expression modules (CMs) were also constructed using the poplar expression data on JA, SA, M. larici-populina, Septoria musiva, and Marssonina brunnea treatment or infection. A total of 23 CMs were constructed, and different CMs clearly exhibited distinct biological functions, which conformably regulated the concerted processes in response to fungal defense. Furthermore, the GO term analysis of different CMs confirmed the roles of JA and SA in regulating growth and defense responses, and their expression profiles suggested that the growth ability was reduced when poplar deployed defense responses. Several transcription factors (TFs) among the CRG in the co-expression network were proposed as hub genes in regulating these processes. According to this study, our data finely uncovered the possible roles of JA/SA in regulating the balance between growth and defense responses by integrating multiple hormone signaling pathways. We were also able to provide more knowledge on how the plant hormones JA/SA are involved in the regulation of the balance between growth and plant defense. |
| first_indexed | 2024-12-10T03:54:43Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-10T03:54:43Z |
| publishDate | 2019-01-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-9a220895af1a4542adc2feba9299c0092022-12-22T02:03:10ZengMDPI AGBiomolecules2218-273X2019-01-01911210.3390/biom9010012biom9010012Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in PoplarJie Luo0Wenxiu Xia1Pei Cao2Zheng’ang Xiao3Yan Zhang4Meifeng Liu5Chang Zhan6Nian Wang7College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaPlants have evolved a sophisticated system to respond to various stresses. Fungal attack or infection is one of the most important biotic stresses for most plants. During the defense response to fungal infection, the plant hormones jasmonic acid (JA) and salicylic acid (SA) play critical roles. Here, gene expression data on JA/SA treatments and Melampsora larici-populina (MLP) infection were generated. Integrated transcriptome analyses of these data were performed, and 943 genes in total were identified as common responsive genes (CRG). Gene ontology (GO) term analysis revealed that the genes from CRG are generally involved in the processes of stress responses, metabolism, and growth and development. The further cluster analysis of the CRG identified a set of core genes that are involved in the JA/SA-mediated response to fungal defense with distinct gene expression profiles upon JA/SA treatment, which highlighted the different effects of these two hormones on plant fungal defenses. The modifications of several pathways relative to metabolism, biotic stress, and plant hormone signal pathways suggest the possible roles of JA/SA on the regulation of growth and defense responses. Co-expression modules (CMs) were also constructed using the poplar expression data on JA, SA, M. larici-populina, Septoria musiva, and Marssonina brunnea treatment or infection. A total of 23 CMs were constructed, and different CMs clearly exhibited distinct biological functions, which conformably regulated the concerted processes in response to fungal defense. Furthermore, the GO term analysis of different CMs confirmed the roles of JA and SA in regulating growth and defense responses, and their expression profiles suggested that the growth ability was reduced when poplar deployed defense responses. Several transcription factors (TFs) among the CRG in the co-expression network were proposed as hub genes in regulating these processes. According to this study, our data finely uncovered the possible roles of JA/SA in regulating the balance between growth and defense responses by integrating multiple hormone signaling pathways. We were also able to provide more knowledge on how the plant hormones JA/SA are involved in the regulation of the balance between growth and plant defense.http://www.mdpi.com/2218-273X/9/1/12poplardefense regulationintegrated transcriptome analysisco-expression network constructionjasmonic acidsalicylic acid |
| spellingShingle | Jie Luo Wenxiu Xia Pei Cao Zheng’ang Xiao Yan Zhang Meifeng Liu Chang Zhan Nian Wang Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar Biomolecules poplar defense regulation integrated transcriptome analysis co-expression network construction jasmonic acid salicylic acid |
| title | Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar |
| title_full | Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar |
| title_fullStr | Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar |
| title_full_unstemmed | Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar |
| title_short | Integrated Transcriptome Analysis Reveals Plant Hormones Jasmonic Acid and Salicylic Acid Coordinate Growth and Defense Responses upon Fungal Infection in Poplar |
| title_sort | integrated transcriptome analysis reveals plant hormones jasmonic acid and salicylic acid coordinate growth and defense responses upon fungal infection in poplar |
| topic | poplar defense regulation integrated transcriptome analysis co-expression network construction jasmonic acid salicylic acid |
| url | http://www.mdpi.com/2218-273X/9/1/12 |
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