<i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach
The nicotinate phosphoribosyltransferase (<i>NAPRT</i>) gene has gained relevance in the research of cancer therapeutic strategies due to its main role as a NAD biosynthetic enzyme. NAD metabolism is an attractive target for the development of anti-cancer therapies, given the high energy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/12/2022 |
| _version_ | 1797504224347553792 |
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| author | Sara Duarte-Pereira Olga Fajarda Sérgio Matos José Luís Oliveira Raquel Monteiro Silva |
| author_facet | Sara Duarte-Pereira Olga Fajarda Sérgio Matos José Luís Oliveira Raquel Monteiro Silva |
| author_sort | Sara Duarte-Pereira |
| collection | DOAJ |
| description | The nicotinate phosphoribosyltransferase (<i>NAPRT</i>) gene has gained relevance in the research of cancer therapeutic strategies due to its main role as a NAD biosynthetic enzyme. NAD metabolism is an attractive target for the development of anti-cancer therapies, given the high energy requirements of proliferating cancer cells and NAD-dependent signaling. A few studies have shown that <i>NAPRT</i> expression varies in different cancer types, making it imperative to assess <i>NAPRT</i> expression and functionality status prior to the application of therapeutic strategies targeting NAD. In addition, the recent finding of NAPRT extracellular form (eNAPRT) suggested the involvement of NAPRT in inflammation and signaling. However, the mechanisms regulating <i>NAPRT</i> gene expression have never been thoroughly addressed. In this study, we searched for <i>NAPRT</i> gene expression regulatory mechanisms in transcription factors (TFs), RNA binding proteins (RBPs) and microRNA (miRNAs) databases. We identified several potential regulators of <i>NAPRT</i> transcription activation, downregulation and alternative splicing and performed GO and expression analyses. The results of the functional analysis of TFs, RBPs and miRNAs suggest new, unexpected functions for the <i>NAPRT</i> gene in cell differentiation, development and neuronal biology. |
| first_indexed | 2024-03-10T04:01:37Z |
| format | Article |
| id | doaj.art-9a227b513c1f479f890fa5cf6a272dc6 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T04:01:37Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-9a227b513c1f479f890fa5cf6a272dc62023-11-23T08:31:49ZengMDPI AGGenes2073-44252021-12-011212202210.3390/genes12122022<i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics ApproachSara Duarte-Pereira0Olga Fajarda1Sérgio Matos2José Luís Oliveira3Raquel Monteiro Silva4Department of Medical Sciences, iBiMED—Institute of Biomedicine, University of Aveiro, 3810-193 Aveiro, PortugalIEETA—Institute of Electronics and Informatics Engineering of Aveiro, University of Aveiro, 3810-193 Aveiro, PortugalIEETA—Institute of Electronics and Informatics Engineering of Aveiro, University of Aveiro, 3810-193 Aveiro, PortugalIEETA—Institute of Electronics and Informatics Engineering of Aveiro, University of Aveiro, 3810-193 Aveiro, PortugalDepartment of Medical Sciences, iBiMED—Institute of Biomedicine, University of Aveiro, 3810-193 Aveiro, PortugalThe nicotinate phosphoribosyltransferase (<i>NAPRT</i>) gene has gained relevance in the research of cancer therapeutic strategies due to its main role as a NAD biosynthetic enzyme. NAD metabolism is an attractive target for the development of anti-cancer therapies, given the high energy requirements of proliferating cancer cells and NAD-dependent signaling. A few studies have shown that <i>NAPRT</i> expression varies in different cancer types, making it imperative to assess <i>NAPRT</i> expression and functionality status prior to the application of therapeutic strategies targeting NAD. In addition, the recent finding of NAPRT extracellular form (eNAPRT) suggested the involvement of NAPRT in inflammation and signaling. However, the mechanisms regulating <i>NAPRT</i> gene expression have never been thoroughly addressed. In this study, we searched for <i>NAPRT</i> gene expression regulatory mechanisms in transcription factors (TFs), RNA binding proteins (RBPs) and microRNA (miRNAs) databases. We identified several potential regulators of <i>NAPRT</i> transcription activation, downregulation and alternative splicing and performed GO and expression analyses. The results of the functional analysis of TFs, RBPs and miRNAs suggest new, unexpected functions for the <i>NAPRT</i> gene in cell differentiation, development and neuronal biology.https://www.mdpi.com/2073-4425/12/12/2022NAPRT (nicotinate phosphoribosyltransferase)bioinformaticsexpression regulationcell differentiationneurodevelopment |
| spellingShingle | Sara Duarte-Pereira Olga Fajarda Sérgio Matos José Luís Oliveira Raquel Monteiro Silva <i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach Genes NAPRT (nicotinate phosphoribosyltransferase) bioinformatics expression regulation cell differentiation neurodevelopment |
| title | <i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach |
| title_full | <i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach |
| title_fullStr | <i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach |
| title_full_unstemmed | <i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach |
| title_short | <i>NAPRT</i> Expression Regulation Mechanisms: Novel Functions Predicted by a Bioinformatics Approach |
| title_sort | i naprt i expression regulation mechanisms novel functions predicted by a bioinformatics approach |
| topic | NAPRT (nicotinate phosphoribosyltransferase) bioinformatics expression regulation cell differentiation neurodevelopment |
| url | https://www.mdpi.com/2073-4425/12/12/2022 |
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