The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients
Abstract Objective The exact aetiology of multiple sclerosis (MS) remains elusive, although several environmental and genetic risk factors have been implicated to varying degrees. Among the environmental risk factors, viral infections have been suggested as strong candidates contributing to MS patho...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-020-05300-1 |
| _version_ | 1818281452856410112 |
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| author | Elie Deeba Anastasia Lambrianides Marios Pantzaris George Krashias Christina Christodoulou |
| author_facet | Elie Deeba Anastasia Lambrianides Marios Pantzaris George Krashias Christina Christodoulou |
| author_sort | Elie Deeba |
| collection | DOAJ |
| description | Abstract Objective The exact aetiology of multiple sclerosis (MS) remains elusive, although several environmental and genetic risk factors have been implicated to varying degrees. Among the environmental risk factors, viral infections have been suggested as strong candidates contributing to MS pathology/progression. Viral recognition and control are largely tasked to the NK cells via TLR recognition and various cytotoxic and immunoregulatory functions. Additionally, the complex roles of different TLRs in MS pathology are highlighted in multiple, often contradictory, studies. The present work aims to analyse the TLR expression profile of NK cells isolated from MS patients. Highly purified CD56+CD3− NK cells isolated from peripheral blood of MS patients (n = 19) and healthy controls (n = 20) were analysed via flow cytometry for their expression of viral antigen-recognizing TLRs (TLR2, TLR3, TLR7, and TLR9). Results No difference was noted in TLR expression between MS patients and healthy controls. These results aim to supplement previous findings which study expressional or functional differences in TLRs present in various subsets of the immune system in MS, thus aiding in a better understanding of MS as a complex multifaceted disease. |
| first_indexed | 2024-12-13T00:05:21Z |
| format | Article |
| id | doaj.art-9a23c065fb7149f8ac3e5c363cccdcb5 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-13T00:05:21Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-9a23c065fb7149f8ac3e5c363cccdcb52022-12-22T00:06:16ZengBMCBMC Research Notes1756-05002020-09-011311710.1186/s13104-020-05300-1The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patientsElie Deeba0Anastasia Lambrianides1Marios Pantzaris2George Krashias3Christina Christodoulou4Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and GeneticsCyprus School of Molecular Medicine, The Cyprus Institute of Neurology and GeneticsCyprus School of Molecular Medicine, The Cyprus Institute of Neurology and GeneticsCyprus School of Molecular Medicine, The Cyprus Institute of Neurology and GeneticsCyprus School of Molecular Medicine, The Cyprus Institute of Neurology and GeneticsAbstract Objective The exact aetiology of multiple sclerosis (MS) remains elusive, although several environmental and genetic risk factors have been implicated to varying degrees. Among the environmental risk factors, viral infections have been suggested as strong candidates contributing to MS pathology/progression. Viral recognition and control are largely tasked to the NK cells via TLR recognition and various cytotoxic and immunoregulatory functions. Additionally, the complex roles of different TLRs in MS pathology are highlighted in multiple, often contradictory, studies. The present work aims to analyse the TLR expression profile of NK cells isolated from MS patients. Highly purified CD56+CD3− NK cells isolated from peripheral blood of MS patients (n = 19) and healthy controls (n = 20) were analysed via flow cytometry for their expression of viral antigen-recognizing TLRs (TLR2, TLR3, TLR7, and TLR9). Results No difference was noted in TLR expression between MS patients and healthy controls. These results aim to supplement previous findings which study expressional or functional differences in TLRs present in various subsets of the immune system in MS, thus aiding in a better understanding of MS as a complex multifaceted disease.http://link.springer.com/article/10.1186/s13104-020-05300-1Multiple sclerosisToll-like receptorFlow cytometryNatural killer cells |
| spellingShingle | Elie Deeba Anastasia Lambrianides Marios Pantzaris George Krashias Christina Christodoulou The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients BMC Research Notes Multiple sclerosis Toll-like receptor Flow cytometry Natural killer cells |
| title | The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients |
| title_full | The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients |
| title_fullStr | The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients |
| title_full_unstemmed | The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients |
| title_short | The expression profile of virus-recognizing toll-like receptors in natural killer cells of Cypriot multiple sclerosis patients |
| title_sort | expression profile of virus recognizing toll like receptors in natural killer cells of cypriot multiple sclerosis patients |
| topic | Multiple sclerosis Toll-like receptor Flow cytometry Natural killer cells |
| url | http://link.springer.com/article/10.1186/s13104-020-05300-1 |
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