Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis
Spermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-04-01
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| Series: | Membranes |
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| Online Access: | http://www.mdpi.com/2077-0375/7/2/22 |
| _version_ | 1797707405752008704 |
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| author | Joana B. Serrano Filipa Martins João C. Sousa Cátia D. Pereira Ans M. M. van Pelt Sandra Rebelo Odete A. B. da Cruz e Silva |
| author_facet | Joana B. Serrano Filipa Martins João C. Sousa Cátia D. Pereira Ans M. M. van Pelt Sandra Rebelo Odete A. B. da Cruz e Silva |
| author_sort | Joana B. Serrano |
| collection | DOAJ |
| description | Spermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated with spermatogenesis. In this study, LAP1 expression and cellular/subcellular localization during spermatogenesis in human and mouse testes is established for the first time. The fact that LAP1 is expressed during nuclear elongation in spermiogenesis and is located at the spermatids’ centriolar pole is singularly important. LAP1 binds to members of the protein phosphatase 1 (PP1) family. Similar localization of LAP1 and PP1γ2, a testis-specific PP1 isoform, suggests a shared function for both proteins during spermiogenesis. Furthermore, this study suggests an involvement of LAP1 in manchette development and chromatin regulation possibly via interaction with acetylated α-tubulin and lamins, respectively. Taken together, the present results indicate that, by moving to the posterior pole in spermatids, LAP1 can contribute to the achievement of non-random, sperm-specific chromatin distribution, as well as modulate cellular remodeling during spermiogenesis. In addition, LAP1 seems to be associated with dynamic microtubule changes related to manchette formation and flagella development. |
| first_indexed | 2024-03-12T06:05:56Z |
| format | Article |
| id | doaj.art-9a23c2635cbb48ed8f84ac8f7610cb53 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T06:05:56Z |
| publishDate | 2017-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-9a23c2635cbb48ed8f84ac8f7610cb532023-09-03T03:37:06ZengMDPI AGMembranes2077-03752017-04-01722210.3390/membranes7020022membranes7020022Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout SpermatogenesisJoana B. Serrano0Filipa Martins1João C. Sousa2Cátia D. Pereira3Ans M. M. van Pelt4Sandra Rebelo5Odete A. B. da Cruz e Silva6Neuroscience and Signaling Laboratory, Department of Medical Sciences, Institute for Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, PortugalNeuroscience and Signaling Laboratory, Department of Medical Sciences, Institute for Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, PortugalLife and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, PortugalNeuroscience and Signaling Laboratory, Department of Medical Sciences, Institute for Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, PortugalCenter for Reproductive Medicine, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The NetherlandsNeuroscience and Signaling Laboratory, Department of Medical Sciences, Institute for Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, PortugalNeuroscience and Signaling Laboratory, Department of Medical Sciences, Institute for Biomedicine—iBiMED, University of Aveiro, 3810-193 Aveiro, PortugalSpermatogenesis comprises highly complex differentiation processes. Nuclear envelope (NE) proteins have been associated with these processes, including lamins, lamina-associated polypeptide (LAP) 2 and the lamin B-receptor. LAP1 is an important NE protein whose function has not been fully elucidated, but several binding partners allow predicting putative LAP1 functions. To date, LAP1 had not been associated with spermatogenesis. In this study, LAP1 expression and cellular/subcellular localization during spermatogenesis in human and mouse testes is established for the first time. The fact that LAP1 is expressed during nuclear elongation in spermiogenesis and is located at the spermatids’ centriolar pole is singularly important. LAP1 binds to members of the protein phosphatase 1 (PP1) family. Similar localization of LAP1 and PP1γ2, a testis-specific PP1 isoform, suggests a shared function for both proteins during spermiogenesis. Furthermore, this study suggests an involvement of LAP1 in manchette development and chromatin regulation possibly via interaction with acetylated α-tubulin and lamins, respectively. Taken together, the present results indicate that, by moving to the posterior pole in spermatids, LAP1 can contribute to the achievement of non-random, sperm-specific chromatin distribution, as well as modulate cellular remodeling during spermiogenesis. In addition, LAP1 seems to be associated with dynamic microtubule changes related to manchette formation and flagella development.http://www.mdpi.com/2077-0375/7/2/22nuclear envelopelamina-associated polypeptide 1laminsprotein phosphatase 1manchettespermatids |
| spellingShingle | Joana B. Serrano Filipa Martins João C. Sousa Cátia D. Pereira Ans M. M. van Pelt Sandra Rebelo Odete A. B. da Cruz e Silva Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis Membranes nuclear envelope lamina-associated polypeptide 1 lamins protein phosphatase 1 manchette spermatids |
| title | Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis |
| title_full | Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis |
| title_fullStr | Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis |
| title_full_unstemmed | Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis |
| title_short | Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis |
| title_sort | descriptive analysis of lap1 distribution and that of associated proteins throughout spermatogenesis |
| topic | nuclear envelope lamina-associated polypeptide 1 lamins protein phosphatase 1 manchette spermatids |
| url | http://www.mdpi.com/2077-0375/7/2/22 |
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