Arachidonic acid triggers an oxidative burst in leukocytes
The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2003-11-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013 |
| _version_ | 1811213789722312704 |
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| author | C. Pompeia M.F. Cury-Boaventura R. Curi |
| author_facet | C. Pompeia M.F. Cury-Boaventura R. Curi |
| author_sort | C. Pompeia |
| collection | DOAJ |
| description | The change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high, decreasing steadily and ceasing about 2 h post-treatment. The continuous presence of AA was not needed to stimulate superoxide generation. It seems that the NADPH oxidase system participates in AA-stimulated superoxide production in these cells since the oxidative burst was stimulated by NADPH and inhibited by N-ethylmaleimide, diphenyleneiodonium and superoxide dismutase. Some of the effects of AA on the oxidative burst may be due to its detergent action. There apparently was no contribution of other superoxide-generating systems such as xanthine-xanthine oxidase, cytochromes P-450 and mitochondrial electron transport chain, as assessed by the use of inhibitors. Eicosanoids and nitric oxide also do not seem to interfere with the AA-stimulated oxidative burst since there was no systematic effect of cyclooxygenase, lipoxygenase or nitric oxide synthase inhibitors, but lipid peroxides may play a role, as indicated by the inhibition of nitroblue tetrazolium reduction promoted by tocopherol. |
| first_indexed | 2024-04-12T05:50:42Z |
| format | Article |
| id | doaj.art-9a28ca225404460e9e38558065db1cf2 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-12T05:50:42Z |
| publishDate | 2003-11-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-9a28ca225404460e9e38558065db1cf22022-12-22T03:45:18ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2003-11-0136111549156010.1590/S0100-879X2003001100013Arachidonic acid triggers an oxidative burst in leukocytesC. PompeiaM.F. Cury-BoaventuraR. CuriThe change in cellular reducing potential, most likely reflecting an oxidative burst, was investigated in arachidonic acid- (AA) stimulated leukocytes. The cells studied included the human leukemia cell lines HL-60 (undifferentiated and differentiated into macrophage-like and polymorphonuclear-like cells), Jurkat and Raji, and thymocytes and macrophages from rat primary cultures. The oxidative burst was assessed by nitroblue tetrazolium reduction. AA increased the oxidative burst until an optimum AA concentration was reached and the burst decreased thereafter. In the leukemia cell lines, optimum concentration ranged from 200 to 400 µM (up to 16-fold), whereas in rat cells it varied from 10 to 20 µM. Initial rates of superoxide generation were high, decreasing steadily and ceasing about 2 h post-treatment. The continuous presence of AA was not needed to stimulate superoxide generation. It seems that the NADPH oxidase system participates in AA-stimulated superoxide production in these cells since the oxidative burst was stimulated by NADPH and inhibited by N-ethylmaleimide, diphenyleneiodonium and superoxide dismutase. Some of the effects of AA on the oxidative burst may be due to its detergent action. There apparently was no contribution of other superoxide-generating systems such as xanthine-xanthine oxidase, cytochromes P-450 and mitochondrial electron transport chain, as assessed by the use of inhibitors. Eicosanoids and nitric oxide also do not seem to interfere with the AA-stimulated oxidative burst since there was no systematic effect of cyclooxygenase, lipoxygenase or nitric oxide synthase inhibitors, but lipid peroxides may play a role, as indicated by the inhibition of nitroblue tetrazolium reduction promoted by tocopherol.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013Arachidonic acidNADPH oxidaseNitroblue tetrazoliumLeukocytesReactive oxygen species |
| spellingShingle | C. Pompeia M.F. Cury-Boaventura R. Curi Arachidonic acid triggers an oxidative burst in leukocytes Brazilian Journal of Medical and Biological Research Arachidonic acid NADPH oxidase Nitroblue tetrazolium Leukocytes Reactive oxygen species |
| title | Arachidonic acid triggers an oxidative burst in leukocytes |
| title_full | Arachidonic acid triggers an oxidative burst in leukocytes |
| title_fullStr | Arachidonic acid triggers an oxidative burst in leukocytes |
| title_full_unstemmed | Arachidonic acid triggers an oxidative burst in leukocytes |
| title_short | Arachidonic acid triggers an oxidative burst in leukocytes |
| title_sort | arachidonic acid triggers an oxidative burst in leukocytes |
| topic | Arachidonic acid NADPH oxidase Nitroblue tetrazolium Leukocytes Reactive oxygen species |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100013 |
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