Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation
The mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed. It causes a disruption of sphingolipid metabolism and pulmonary, hepatic, and immunological lesions in pigs depending on the exposure scenario. One sensitive biomarker for FB1 exposure is the sphinganine (Sa) to sphingosine (So) rat...
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MDPI AG
2018-07-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/7/296 |
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| author | Hanna Schertz Sven Dänicke Jana Frahm Dian Schatzmayr Ilse Dohnal Gerlinde Bichl Heidi E. Schwartz-Zimmermann Sonia Colicchia Gerhard Breves Jens P. Teifke Jeannette Kluess |
| author_facet | Hanna Schertz Sven Dänicke Jana Frahm Dian Schatzmayr Ilse Dohnal Gerlinde Bichl Heidi E. Schwartz-Zimmermann Sonia Colicchia Gerhard Breves Jens P. Teifke Jeannette Kluess |
| author_sort | Hanna Schertz |
| collection | DOAJ |
| description | The mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed. It causes a disruption of sphingolipid metabolism and pulmonary, hepatic, and immunological lesions in pigs depending on the exposure scenario. One sensitive biomarker for FB1 exposure is the sphinganine (Sa) to sphingosine (So) ratio in blood. The fumonisin esterase FumD, which can be used as a feed additive, converts FB1 into the much less toxic metabolite hydrolyzed FB1 (HFB1). We conducted a single-dose study with barrows allocated to one of five treatments: (1) control (feed, 0.9% NaCl intravenously iv), (2) 139 nmol FB1 or (3) HFB1/kg BW iv, (4) 3425 nmol FB1/kg BW orally (po), or (5) 3321 nmol FB1/kg BW and 240 U FumD/kg feed po. The Sa/So ratio of iv and po FB1 administered groups was significantly elevated in blood and Liquor cerebrospinalis, but no fumonisin-associated differences were reflected in other endpoints. Neither clinical lung affections nor histopathological pulmonary lesions were detected in either group, while some parameters of hematology and clinical biochemistry showed a treatment–time interaction. FumD application resulted in Sa/So ratios comparable to the control, indicating that the enzymatic treatment was effectively preventing the fumonisin-induced disruption of sphingolipid metabolism. |
| first_indexed | 2024-04-11T12:49:59Z |
| format | Article |
| id | doaj.art-9a29ef22ae124a29a4afeb05271b8b25 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T12:49:59Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-9a29ef22ae124a29a4afeb05271b8b252022-12-22T04:23:14ZengMDPI AGToxins2072-66512018-07-0110729610.3390/toxins10070296toxins10070296Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase SupplementationHanna Schertz0Sven Dänicke1Jana Frahm2Dian Schatzmayr3Ilse Dohnal4Gerlinde Bichl5Heidi E. Schwartz-Zimmermann6Sonia Colicchia7Gerhard Breves8Jens P. Teifke9Jeannette Kluess10Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 38116 Braunschweig, GermanyFriedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 38116 Braunschweig, GermanyFriedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 38116 Braunschweig, GermanyBIOMIN Holding GmbH, BIOMIN Research Center, 3430 Tulln, AustriaBIOMIN Holding GmbH, BIOMIN Research Center, 3430 Tulln, AustriaBIOMIN Holding GmbH, BIOMIN Research Center, 3430 Tulln, AustriaChristian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, IFA, 3430 Tulln, AustriaChristian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, IFA, 3430 Tulln, AustriaInstitute for Physiology, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, GermanyFriedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riems, GermanyFriedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 38116 Braunschweig, GermanyThe mycotoxin fumonisin B1 (FB1) is a frequent contaminant of feed. It causes a disruption of sphingolipid metabolism and pulmonary, hepatic, and immunological lesions in pigs depending on the exposure scenario. One sensitive biomarker for FB1 exposure is the sphinganine (Sa) to sphingosine (So) ratio in blood. The fumonisin esterase FumD, which can be used as a feed additive, converts FB1 into the much less toxic metabolite hydrolyzed FB1 (HFB1). We conducted a single-dose study with barrows allocated to one of five treatments: (1) control (feed, 0.9% NaCl intravenously iv), (2) 139 nmol FB1 or (3) HFB1/kg BW iv, (4) 3425 nmol FB1/kg BW orally (po), or (5) 3321 nmol FB1/kg BW and 240 U FumD/kg feed po. The Sa/So ratio of iv and po FB1 administered groups was significantly elevated in blood and Liquor cerebrospinalis, but no fumonisin-associated differences were reflected in other endpoints. Neither clinical lung affections nor histopathological pulmonary lesions were detected in either group, while some parameters of hematology and clinical biochemistry showed a treatment–time interaction. FumD application resulted in Sa/So ratios comparable to the control, indicating that the enzymatic treatment was effectively preventing the fumonisin-induced disruption of sphingolipid metabolism.http://www.mdpi.com/2072-6651/10/7/296fumonisinpigsSa/So ratiosingle-doseclinical examinationblood countclinical biochemistryfumonisin esterase |
| spellingShingle | Hanna Schertz Sven Dänicke Jana Frahm Dian Schatzmayr Ilse Dohnal Gerlinde Bichl Heidi E. Schwartz-Zimmermann Sonia Colicchia Gerhard Breves Jens P. Teifke Jeannette Kluess Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation Toxins fumonisin pigs Sa/So ratio single-dose clinical examination blood count clinical biochemistry fumonisin esterase |
| title | Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation |
| title_full | Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation |
| title_fullStr | Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation |
| title_full_unstemmed | Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation |
| title_short | Biomarker Evaluation and Toxic Effects of an Acute Oral and Systemic Fumonisin Exposure of Pigs with a Special Focus on Dietary Fumonisin Esterase Supplementation |
| title_sort | biomarker evaluation and toxic effects of an acute oral and systemic fumonisin exposure of pigs with a special focus on dietary fumonisin esterase supplementation |
| topic | fumonisin pigs Sa/So ratio single-dose clinical examination blood count clinical biochemistry fumonisin esterase |
| url | http://www.mdpi.com/2072-6651/10/7/296 |
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