NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines
In the search of new natural products to be explored as possible anticancer drugs, two plant species, namely <i>Ononis diffusa</i> and <i>Ononis variegata</i>, were screened against colorectal cancer cell lines. The cytotoxic activity of the crude extracts was tested on a pan...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/11/3266 |
| _version_ | 1827691219198672896 |
|---|---|
| author | Vittoria Graziani Nicoletta Potenza Brigida D’Abrosca Teresa Troiani Stefania Napolitano Antonio Fiorentino Monica Scognamiglio |
| author_facet | Vittoria Graziani Nicoletta Potenza Brigida D’Abrosca Teresa Troiani Stefania Napolitano Antonio Fiorentino Monica Scognamiglio |
| author_sort | Vittoria Graziani |
| collection | DOAJ |
| description | In the search of new natural products to be explored as possible anticancer drugs, two plant species, namely <i>Ononis diffusa</i> and <i>Ononis variegata</i>, were screened against colorectal cancer cell lines. The cytotoxic activity of the crude extracts was tested on a panel of colon cancer cell models including cetuximab-sensitive (Caco-2, GEO, SW48), intrinsic (HT-29 and HCT-116), and acquired (GEO-CR, SW48-CR) cetuximab-resistant cell lines. <i>Ononis diffusa</i> showed remarkable cytotoxic activity, especially on the cetuximab-resistant cell lines. The active extract composition was determined by NMR analysis. Given its complexity, a partial purification was then carried out. The fractions obtained were again tested for their biological activity and their metabolite content was determined by 1D and 2D NMR analysis. The study led to the identification of a fraction enriched in oxylipins that showed a 92% growth inhibition of the HT-29 cell line at a concentration of 50 µg/mL. |
| first_indexed | 2024-03-10T10:55:49Z |
| format | Article |
| id | doaj.art-9a2dfe936e4d4933976efc9fd323c8ff |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T10:55:49Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9a2dfe936e4d4933976efc9fd323c8ff2023-11-21T21:53:52ZengMDPI AGMolecules1420-30492021-05-012611326610.3390/molecules26113266NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell LinesVittoria Graziani0Nicoletta Potenza1Brigida D’Abrosca2Teresa Troiani3Stefania Napolitano4Antonio Fiorentino5Monica Scognamiglio6Dipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli Studi della Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli Studi della Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli Studi della Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyOncologia medica, Dipartimento di Medicina di precisione, Università degli Studi della Campania “Luigi Vanvitelli”, S. Andrea delle Dame, Via L. De Crecchio 7, 80138 Napoli, ItalyOncologia medica, Dipartimento di Medicina di precisione, Università degli Studi della Campania “Luigi Vanvitelli”, S. Andrea delle Dame, Via L. De Crecchio 7, 80138 Napoli, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli Studi della Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyDipartimento di Scienze e Tecnologie Ambientali, Biologiche e Farmaceutiche, Università degli Studi della Campania “Luigi Vanvitelli”, Via Vivaldi 43, 81100 Caserta, ItalyIn the search of new natural products to be explored as possible anticancer drugs, two plant species, namely <i>Ononis diffusa</i> and <i>Ononis variegata</i>, were screened against colorectal cancer cell lines. The cytotoxic activity of the crude extracts was tested on a panel of colon cancer cell models including cetuximab-sensitive (Caco-2, GEO, SW48), intrinsic (HT-29 and HCT-116), and acquired (GEO-CR, SW48-CR) cetuximab-resistant cell lines. <i>Ononis diffusa</i> showed remarkable cytotoxic activity, especially on the cetuximab-resistant cell lines. The active extract composition was determined by NMR analysis. Given its complexity, a partial purification was then carried out. The fractions obtained were again tested for their biological activity and their metabolite content was determined by 1D and 2D NMR analysis. The study led to the identification of a fraction enriched in oxylipins that showed a 92% growth inhibition of the HT-29 cell line at a concentration of 50 µg/mL.https://www.mdpi.com/1420-3049/26/11/3266cytotoxic activitynatural productsNMR<i>Ononis diffusa</i><i>Ononis variegata</i>oxylipins |
| spellingShingle | Vittoria Graziani Nicoletta Potenza Brigida D’Abrosca Teresa Troiani Stefania Napolitano Antonio Fiorentino Monica Scognamiglio NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines Molecules cytotoxic activity natural products NMR <i>Ononis diffusa</i> <i>Ononis variegata</i> oxylipins |
| title | NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines |
| title_full | NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines |
| title_fullStr | NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines |
| title_full_unstemmed | NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines |
| title_short | NMR Profiling of <i>Ononis diffusa</i> Identifies Cytotoxic Compounds against Cetuximab-Resistant Colon Cancer Cell Lines |
| title_sort | nmr profiling of i ononis diffusa i identifies cytotoxic compounds against cetuximab resistant colon cancer cell lines |
| topic | cytotoxic activity natural products NMR <i>Ononis diffusa</i> <i>Ononis variegata</i> oxylipins |
| url | https://www.mdpi.com/1420-3049/26/11/3266 |
| work_keys_str_mv | AT vittoriagraziani nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines AT nicolettapotenza nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines AT brigidadabrosca nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines AT teresatroiani nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines AT stefanianapolitano nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines AT antoniofiorentino nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines AT monicascognamiglio nmrprofilingofiononisdiffusaiidentifiescytotoxiccompoundsagainstcetuximabresistantcoloncancercelllines |