Protocol for callus induction and somatic embryogenesis in Moso Bamboo.
Moso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicate...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3859595?pdf=render |
| _version_ | 1818117545955164160 |
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| author | Jin-Ling Yuan Jin-Jun Yue Xiao-Li Wu Xiao-Ping Gu |
| author_facet | Jin-Ling Yuan Jin-Jun Yue Xiao-Li Wu Xiao-Ping Gu |
| author_sort | Jin-Ling Yuan |
| collection | DOAJ |
| description | Moso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicated by callus induction for monopodial bamboos, such as Moso bamboo. In the present report, embryogenic callus induction, embryoid development, and germination were established for Moso bamboo from zygotic seed embryos. Callus was initiated from zygotic embryos after 10-20 d culture on MS media supplemented with 4.0 mg/L 2, 4-D and 0.1 mg/L zeatin (ZT). About 50% of the explants produced calli, and nearly 15% of the calli were found to be embryogenic in nature. These embryogenic calli can be subcultured for proliferation in the Murashige and Skoog media (MS) supplemented with 0.5-2.0 mg/L 2, 4-D. These calli were found to have maintained their capacity for regeneration even after one year of subculture. The viable somatic embryoids regenerated in medium containing 5.0-7.0 mg/L ZT. Nearly 5% of the calli were found capable of regenerating into plantlets directly in MS medium containing 5.0-7.0 mg/L ZT. Root growth was more pronounced when the plantlets were transferred to medium containing 2.0 mg/L NAA. After 30 days of subculture, the plantlets were transferred to a greenhouse. |
| first_indexed | 2024-12-11T04:40:07Z |
| format | Article |
| id | doaj.art-9a2f831afa15444c88a3d666a285479a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T04:40:07Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9a2f831afa15444c88a3d666a285479a2022-12-22T01:20:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8195410.1371/journal.pone.0081954Protocol for callus induction and somatic embryogenesis in Moso Bamboo.Jin-Ling YuanJin-Jun YueXiao-Li WuXiao-Ping GuMoso bamboo [Phyllostachys heterocycla var. pubescens (Mazel ex J. Houz.) Ohwi] is one of the most important forest crops in China and the rest of Asia. Although many sympodial bamboo tissue culture protocols have been established, there is no protocol available for plantlet regeneration as indicated by callus induction for monopodial bamboos, such as Moso bamboo. In the present report, embryogenic callus induction, embryoid development, and germination were established for Moso bamboo from zygotic seed embryos. Callus was initiated from zygotic embryos after 10-20 d culture on MS media supplemented with 4.0 mg/L 2, 4-D and 0.1 mg/L zeatin (ZT). About 50% of the explants produced calli, and nearly 15% of the calli were found to be embryogenic in nature. These embryogenic calli can be subcultured for proliferation in the Murashige and Skoog media (MS) supplemented with 0.5-2.0 mg/L 2, 4-D. These calli were found to have maintained their capacity for regeneration even after one year of subculture. The viable somatic embryoids regenerated in medium containing 5.0-7.0 mg/L ZT. Nearly 5% of the calli were found capable of regenerating into plantlets directly in MS medium containing 5.0-7.0 mg/L ZT. Root growth was more pronounced when the plantlets were transferred to medium containing 2.0 mg/L NAA. After 30 days of subculture, the plantlets were transferred to a greenhouse.http://europepmc.org/articles/PMC3859595?pdf=render |
| spellingShingle | Jin-Ling Yuan Jin-Jun Yue Xiao-Li Wu Xiao-Ping Gu Protocol for callus induction and somatic embryogenesis in Moso Bamboo. PLoS ONE |
| title | Protocol for callus induction and somatic embryogenesis in Moso Bamboo. |
| title_full | Protocol for callus induction and somatic embryogenesis in Moso Bamboo. |
| title_fullStr | Protocol for callus induction and somatic embryogenesis in Moso Bamboo. |
| title_full_unstemmed | Protocol for callus induction and somatic embryogenesis in Moso Bamboo. |
| title_short | Protocol for callus induction and somatic embryogenesis in Moso Bamboo. |
| title_sort | protocol for callus induction and somatic embryogenesis in moso bamboo |
| url | http://europepmc.org/articles/PMC3859595?pdf=render |
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