Optimize nearly PAM-less adenine base editor to improve editing efficiency

Optimize nearly PAM-less adenine base editor to improve editing efficiency

Objective To explore the effects of replacing different components of PAM-less adenine base editor (ABE) on its editing efficiency. Methods ABEmax was replaced with Tad-8e in SpRY-ABEmax to construct SpRY-ABE8e. Then the connecting peptide 3xGGS-XTEN-3xGGS between Cas9 protein variant SpRY and Tad-8...

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Bibliographic Details
Main Author:	ZHOU Xiao-yu, WANG Xiao-yue
Format:	Article
Language:	zho
Published:	Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2022-06-01
Series:	Jichu yixue yu linchuang
Subjects:	adenine base editor\|spry\|abe8e\|editing efficiency
Online Access:	http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-902.pdf

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