Optimize nearly PAM-less adenine base editor to improve editing efficiency
Objective To explore the effects of replacing different components of PAM-less adenine base editor (ABE) on its editing efficiency. Methods ABEmax was replaced with Tad-8e in SpRY-ABEmax to construct SpRY-ABE8e. Then the connecting peptide 3xGGS-XTEN-3xGGS between Cas9 protein variant SpRY and Tad-8...
| Hlavní autor: | |
|---|---|
| Médium: | Článek |
| Jazyk: | zho |
| Vydáno: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-06-01
|
| Edice: | Jichu yixue yu linchuang |
| Témata: | |
| On-line přístup: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-902.pdf |