Optimize nearly PAM-less adenine base editor to improve editing efficiency

Optimize nearly PAM-less adenine base editor to improve editing efficiency

Objective To explore the effects of replacing different components of PAM-less adenine base editor (ABE) on its editing efficiency. Methods ABEmax was replaced with Tad-8e in SpRY-ABEmax to construct SpRY-ABE8e. Then the connecting peptide 3xGGS-XTEN-3xGGS between Cas9 protein variant SpRY and Tad-8...

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书目详细资料
主要作者: ZHOU Xiao-yu, WANG Xiao-yue
格式: 文件
语言:zho
出版: Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2022-06-01
丛编:Jichu yixue yu linchuang
主题:
adenine base editor|spry|abe8e|editing efficiency
在线阅读:http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-902.pdf

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http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-6-902.pdf

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