Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC
The causative agent of Legionnaires’ disease, Legionella pneumophila, delivers more than 330 virulent effectors to its host to establish an intracellular membrane-bound organelle called the Legionella containing vacuole. Among the army of Legionella effectors, SidC and its paralog SdcA have been ide...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/36154 |
| _version_ | 1811252947760185344 |
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| author | David Jon Wasilko Qingqiu Huang Yuxin Mao |
| author_facet | David Jon Wasilko Qingqiu Huang Yuxin Mao |
| author_sort | David Jon Wasilko |
| collection | DOAJ |
| description | The causative agent of Legionnaires’ disease, Legionella pneumophila, delivers more than 330 virulent effectors to its host to establish an intracellular membrane-bound organelle called the Legionella containing vacuole. Among the army of Legionella effectors, SidC and its paralog SdcA have been identified as novel bacterial ubiquitin (Ub) E3 ligases. To gain insight into the molecular mechanism of SidC/SdcA as Ub ligases, we determined the crystal structures of a binary complex of the N-terminal catalytic SNL domain of SdcA with its cognate E2 UbcH5C and a ternary complex consisting of the SNL domain of SidC with the Ub-linked E2 UbcH7. These two structures reveal the molecular determinants governing the Ub transfer cascade catalyzed by SidC. Together, our data support a common mechanism in the Ub transfer cascade in which the donor Ub is immobilized with its C-terminal tail locked in an extended conformation, priming the donor Ub for catalysis. |
| first_indexed | 2024-04-12T16:43:28Z |
| format | Article |
| id | doaj.art-9a3213f177ec463faaf1f5add6a7b0b7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:43:28Z |
| publishDate | 2018-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9a3213f177ec463faaf1f5add6a7b0b72022-12-22T03:24:41ZengeLife Sciences Publications LtdeLife2050-084X2018-07-01710.7554/eLife.36154Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidCDavid Jon Wasilko0https://orcid.org/0000-0002-6227-5902Qingqiu Huang1Yuxin Mao2https://orcid.org/0000-0002-5064-1397Department of Molecular Biology and Genetics, Cornell University, Ithaca, United States; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, United StatesMacCHESS, Cornell University, Ithaca, United StatesDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, United States; Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, United StatesThe causative agent of Legionnaires’ disease, Legionella pneumophila, delivers more than 330 virulent effectors to its host to establish an intracellular membrane-bound organelle called the Legionella containing vacuole. Among the army of Legionella effectors, SidC and its paralog SdcA have been identified as novel bacterial ubiquitin (Ub) E3 ligases. To gain insight into the molecular mechanism of SidC/SdcA as Ub ligases, we determined the crystal structures of a binary complex of the N-terminal catalytic SNL domain of SdcA with its cognate E2 UbcH5C and a ternary complex consisting of the SNL domain of SidC with the Ub-linked E2 UbcH7. These two structures reveal the molecular determinants governing the Ub transfer cascade catalyzed by SidC. Together, our data support a common mechanism in the Ub transfer cascade in which the donor Ub is immobilized with its C-terminal tail locked in an extended conformation, priming the donor Ub for catalysis.https://elifesciences.org/articles/36154HECT E3 ligaselegionella pneumophilaUbcH5UbcH7 |
| spellingShingle | David Jon Wasilko Qingqiu Huang Yuxin Mao Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC eLife HECT E3 ligase legionella pneumophila UbcH5 UbcH7 |
| title | Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC |
| title_full | Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC |
| title_fullStr | Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC |
| title_full_unstemmed | Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC |
| title_short | Insights into the ubiquitin transfer cascade catalyzed by the Legionella effector SidC |
| title_sort | insights into the ubiquitin transfer cascade catalyzed by the legionella effector sidc |
| topic | HECT E3 ligase legionella pneumophila UbcH5 UbcH7 |
| url | https://elifesciences.org/articles/36154 |
| work_keys_str_mv | AT davidjonwasilko insightsintotheubiquitintransfercascadecatalyzedbythelegionellaeffectorsidc AT qingqiuhuang insightsintotheubiquitintransfercascadecatalyzedbythelegionellaeffectorsidc AT yuxinmao insightsintotheubiquitintransfercascadecatalyzedbythelegionellaeffectorsidc |