Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen
In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distrib...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2016-12-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/16/12/2154 |
| _version_ | 1818037039003598848 |
|---|---|
| author | Dong Seok Kim Yong Tae Kim Seok Bok Hong Jinwoon Kim Nam Su Heo Moon-Keun Lee Seok Jae Lee Byeong Il Kim In Soo Kim Yun Suk Huh Bong Gill Choi |
| author_facet | Dong Seok Kim Yong Tae Kim Seok Bok Hong Jinwoon Kim Nam Su Heo Moon-Keun Lee Seok Jae Lee Byeong Il Kim In Soo Kim Yun Suk Huh Bong Gill Choi |
| author_sort | Dong Seok Kim |
| collection | DOAJ |
| description | In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs. |
| first_indexed | 2024-12-10T07:20:30Z |
| format | Article |
| id | doaj.art-9a3419fea7124fdbb798a71b98d1bbde |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T07:20:30Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9a3419fea7124fdbb798a71b98d1bbde2022-12-22T01:57:49ZengMDPI AGSensors1424-82202016-12-011612215410.3390/s16122154s16122154Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface AntigenDong Seok Kim0Yong Tae Kim1Seok Bok Hong2Jinwoon Kim3Nam Su Heo4Moon-Keun Lee5Seok Jae Lee6Byeong Il Kim7In Soo Kim8Yun Suk Huh9Bong Gill Choi10Department of Chemical Engineering, Kangwon National University, Samcheok 25913, KoreaNanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, KoreaDepartment of Chemical Engineering, Kangwon National University, Samcheok 25913, KoreaDepartment of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, KoreaDepartment of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, KoreaNanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, KoreaNanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, KoreaResearch and Development Center, Tomorrow & Solution (T&S), E19-604, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, KoreaResearch Center 2, Eudipia Inc., Cheongju 361-951, KoreaDepartment of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, KoreaDepartment of Chemical Engineering, Kangwon National University, Samcheok 25913, KoreaIn this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342–137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.http://www.mdpi.com/1424-8220/16/12/2154goldnanoparticlehepatitis B surface antigenlateral flow assayconjugation |
| spellingShingle | Dong Seok Kim Yong Tae Kim Seok Bok Hong Jinwoon Kim Nam Su Heo Moon-Keun Lee Seok Jae Lee Byeong Il Kim In Soo Kim Yun Suk Huh Bong Gill Choi Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen Sensors gold nanoparticle hepatitis B surface antigen lateral flow assay conjugation |
| title | Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen |
| title_full | Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen |
| title_fullStr | Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen |
| title_full_unstemmed | Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen |
| title_short | Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen |
| title_sort | development of lateral flow assay based on size controlled gold nanoparticles for detection of hepatitis b surface antigen |
| topic | gold nanoparticle hepatitis B surface antigen lateral flow assay conjugation |
| url | http://www.mdpi.com/1424-8220/16/12/2154 |
| work_keys_str_mv | AT dongseokkim developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT yongtaekim developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT seokbokhong developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT jinwoonkim developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT namsuheo developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT moonkeunlee developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT seokjaelee developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT byeongilkim developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT insookim developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT yunsukhuh developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen AT bonggillchoi developmentoflateralflowassaybasedonsizecontrolledgoldnanoparticlesfordetectionofhepatitisbsurfaceantigen |