Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner
Estrogen receptor alpha (ERα) activation by estrogens prevents atheroma through its nuclear action, whereas plasma membrane-located ERα accelerates endothelial healing. The genetic deficiency of ERα was associated with a reduction in flow-mediated dilation (FMD) in one man. Here, we evaluated ex viv...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-11-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/68695 |
| _version_ | 1818026725539315712 |
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| author | Julie Favre Emilie Vessieres Anne-Laure Guihot Coralyne Proux Linda Grimaud Jordan Rivron Manuela CL Garcia Léa Réthoré Rana Zahreddine Morgane Davezac Chanaelle Fébrissy Marine Adlanmerini Laurent Loufrani Vincent Procaccio Jean-Michel Foidart Gilles Flouriot Françoise Lenfant Coralie Fontaine Jean-François Arnal Daniel Henrion |
| author_facet | Julie Favre Emilie Vessieres Anne-Laure Guihot Coralyne Proux Linda Grimaud Jordan Rivron Manuela CL Garcia Léa Réthoré Rana Zahreddine Morgane Davezac Chanaelle Fébrissy Marine Adlanmerini Laurent Loufrani Vincent Procaccio Jean-Michel Foidart Gilles Flouriot Françoise Lenfant Coralie Fontaine Jean-François Arnal Daniel Henrion |
| author_sort | Julie Favre |
| collection | DOAJ |
| description | Estrogen receptor alpha (ERα) activation by estrogens prevents atheroma through its nuclear action, whereas plasma membrane-located ERα accelerates endothelial healing. The genetic deficiency of ERα was associated with a reduction in flow-mediated dilation (FMD) in one man. Here, we evaluated ex vivo the role of ERα on FMD of resistance arteries. FMD, but not agonist (acetylcholine, insulin)-mediated dilation, was reduced in male and female mice lacking ERα (Esr1-/- mice) compared to wild-type mice and was not dependent on the presence of estrogens. In C451A-ERα mice lacking membrane ERα, not in mice lacking AF2-dependent nuclear ERα actions, FMD was reduced, and restored by antioxidant treatments. Compared to wild-type mice, isolated perfused kidneys of C451A-ERα mice revealed a decreased flow-mediated nitrate production and an increased H2O2 production. Thus, endothelial membrane ERα promotes NO bioavailability through inhibition of oxidative stress and thereby participates in FMD in a ligand-independent manner. |
| first_indexed | 2024-12-10T04:36:34Z |
| format | Article |
| id | doaj.art-9a36969e61414a318a017f8e1eddcd91 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T04:36:34Z |
| publishDate | 2021-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-9a36969e61414a318a017f8e1eddcd912022-12-22T02:01:59ZengeLife Sciences Publications LtdeLife2050-084X2021-11-011010.7554/eLife.68695Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent mannerJulie Favre0Emilie Vessieres1Anne-Laure Guihot2Coralyne Proux3Linda Grimaud4Jordan Rivron5Manuela CL Garcia6Léa Réthoré7Rana Zahreddine8Morgane Davezac9Chanaelle Fébrissy10Marine Adlanmerini11Laurent Loufrani12https://orcid.org/0000-0003-3397-2335Vincent Procaccio13Jean-Michel Foidart14Gilles Flouriot15Françoise Lenfant16Coralie Fontaine17Jean-François Arnal18Daniel Henrion19https://orcid.org/0000-0003-1094-0285Angers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; CARFI facility, Angers University, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; CARFI facility, Angers University, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; CARFI facility, Angers University, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; CARFI facility, Angers University, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; CARFI facility, Angers University, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; University Hospital (CHU) of Angers, Angers, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; University Hospital (CHU) of Angers, Angers, FranceGroupe Interdisciplinaire de Génoprotéomique Appliquée, Université de Liège, Liège, BelgiumINSERM U1085, IRSET (Institut de Recherche en Santé, Environnement et Travail), University of Rennes, Rennes, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceINSERM U1297, Paul Sabatier University (Toulouse III) , University Hospital (UHC) of Toulouse, Toulouse, FranceAngers University, MITOVASC, CNRS UMR 6015, INSERM U1083, Angers, France; CARFI facility, Angers University, Angers, France; University Hospital (CHU) of Angers, Angers, FranceEstrogen receptor alpha (ERα) activation by estrogens prevents atheroma through its nuclear action, whereas plasma membrane-located ERα accelerates endothelial healing. The genetic deficiency of ERα was associated with a reduction in flow-mediated dilation (FMD) in one man. Here, we evaluated ex vivo the role of ERα on FMD of resistance arteries. FMD, but not agonist (acetylcholine, insulin)-mediated dilation, was reduced in male and female mice lacking ERα (Esr1-/- mice) compared to wild-type mice and was not dependent on the presence of estrogens. In C451A-ERα mice lacking membrane ERα, not in mice lacking AF2-dependent nuclear ERα actions, FMD was reduced, and restored by antioxidant treatments. Compared to wild-type mice, isolated perfused kidneys of C451A-ERα mice revealed a decreased flow-mediated nitrate production and an increased H2O2 production. Thus, endothelial membrane ERα promotes NO bioavailability through inhibition of oxidative stress and thereby participates in FMD in a ligand-independent manner.https://elifesciences.org/articles/68695endotheliumshear stressestrogen receptorsblood flowresistance arteries |
| spellingShingle | Julie Favre Emilie Vessieres Anne-Laure Guihot Coralyne Proux Linda Grimaud Jordan Rivron Manuela CL Garcia Léa Réthoré Rana Zahreddine Morgane Davezac Chanaelle Fébrissy Marine Adlanmerini Laurent Loufrani Vincent Procaccio Jean-Michel Foidart Gilles Flouriot Françoise Lenfant Coralie Fontaine Jean-François Arnal Daniel Henrion Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner eLife endothelium shear stress estrogen receptors blood flow resistance arteries |
| title | Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner |
| title_full | Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner |
| title_fullStr | Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner |
| title_full_unstemmed | Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner |
| title_short | Membrane estrogen receptor alpha (ERα) participates in flow-mediated dilation in a ligand-independent manner |
| title_sort | membrane estrogen receptor alpha erα participates in flow mediated dilation in a ligand independent manner |
| topic | endothelium shear stress estrogen receptors blood flow resistance arteries |
| url | https://elifesciences.org/articles/68695 |
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