Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels
The normal methods of agricultural production worldwide have been strongly affected by the frequent occurrence of drought. Rice rhizosphere microorganisms have been significantly affected by drought stress. To provide a hypothetical basis for improving the drought resistance and N utilization effici...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1120584/full |
| _version_ | 1797851661704626176 |
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| author | Changhui Sun Changhui Sun Runnan Wang Runnan Wang Guoping Tang Shuo Cai Hong Shi Fangping Liu Hengwang Xie Jinyan Zhu Jinyan Zhu Jinyan Zhu Qiangqiang Xiong Qiangqiang Xiong Qiangqiang Xiong Qiangqiang Xiong |
| author_facet | Changhui Sun Changhui Sun Runnan Wang Runnan Wang Guoping Tang Shuo Cai Hong Shi Fangping Liu Hengwang Xie Jinyan Zhu Jinyan Zhu Jinyan Zhu Qiangqiang Xiong Qiangqiang Xiong Qiangqiang Xiong Qiangqiang Xiong |
| author_sort | Changhui Sun |
| collection | DOAJ |
| description | The normal methods of agricultural production worldwide have been strongly affected by the frequent occurrence of drought. Rice rhizosphere microorganisms have been significantly affected by drought stress. To provide a hypothetical basis for improving the drought resistance and N utilization efficiency of rice, the study adopted a barrel planting method at the heading stage, treating rice with no drought or drought stress and three different nitrogen (N) levels. Untargeted metabolomics and 16S rRNA gene sequencing technology were used to study the changes in microorganisms in roots and the differential metabolites (DMs) in rhizosphere soil. The results showed that under the same N application rate, the dry matter mass, N content and N accumulation in rice plants increased to different degrees under drought stress. The root soluble protein, nitrate reductase and soil urease activities were improved over those of the no-drought treatment. Proteobacteria, Bacteroidota, Nitrospirota and Zixibacteria were the dominant flora related to N absorption. A total of 184 DMs (98 upregulated and 86 downregulated) were identified between low N with no drought (LN) and normal N with no drought (NN); 139 DMs (83 upregulated and 56 downregulated) were identified between high N with no drought (HN) and NN; 166 DMs (103 upregulated and 63 downregulated) were identified between low N with drought stress (LND) and normal N with drought stress (NND); and 124 DMs (71 upregulated and 53 downregulated) were identified between high N with drought stress (HND) and NND. Fatty acyl was the metabolite with the highest proportion. KEGG analysis showed that energy metabolism pathways, such as D-alanine metabolism and the phosphotransferase system (PTS), were enriched. We conclude that N-metabolism enzymes with higher activity and higher bacterial diversity have a significant effect on drought tolerance and nitrogen uptake in rice. |
| first_indexed | 2024-04-09T19:21:20Z |
| format | Article |
| id | doaj.art-9a3829d4eca3445db42864cc325dfbfd |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T19:21:20Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-9a3829d4eca3445db42864cc325dfbfd2023-04-05T13:39:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-04-011410.3389/fpls.2023.11205841120584Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levelsChanghui Sun0Changhui Sun1Runnan Wang2Runnan Wang3Guoping Tang4Shuo Cai5Hong Shi6Fangping Liu7Hengwang Xie8Jinyan Zhu9Jinyan Zhu10Jinyan Zhu11Qiangqiang Xiong12Qiangqiang Xiong13Qiangqiang Xiong14Qiangqiang Xiong15Jiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangxi Academy of Agricultural Sciences Rice Research Institute, Nanchang, ChinaJiangxi Irrigation Experiment Central Station, Nanchang, ChinaJiangxi Irrigation Experiment Central Station, Nanchang, ChinaJiangxi Irrigation Experiment Central Station, Nanchang, ChinaJiangxi Irrigation Experiment Central Station, Nanchang, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, ChinaJiangxi Irrigation Experiment Central Station, Nanchang, ChinaJiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, ChinaThe normal methods of agricultural production worldwide have been strongly affected by the frequent occurrence of drought. Rice rhizosphere microorganisms have been significantly affected by drought stress. To provide a hypothetical basis for improving the drought resistance and N utilization efficiency of rice, the study adopted a barrel planting method at the heading stage, treating rice with no drought or drought stress and three different nitrogen (N) levels. Untargeted metabolomics and 16S rRNA gene sequencing technology were used to study the changes in microorganisms in roots and the differential metabolites (DMs) in rhizosphere soil. The results showed that under the same N application rate, the dry matter mass, N content and N accumulation in rice plants increased to different degrees under drought stress. The root soluble protein, nitrate reductase and soil urease activities were improved over those of the no-drought treatment. Proteobacteria, Bacteroidota, Nitrospirota and Zixibacteria were the dominant flora related to N absorption. A total of 184 DMs (98 upregulated and 86 downregulated) were identified between low N with no drought (LN) and normal N with no drought (NN); 139 DMs (83 upregulated and 56 downregulated) were identified between high N with no drought (HN) and NN; 166 DMs (103 upregulated and 63 downregulated) were identified between low N with drought stress (LND) and normal N with drought stress (NND); and 124 DMs (71 upregulated and 53 downregulated) were identified between high N with drought stress (HND) and NND. Fatty acyl was the metabolite with the highest proportion. KEGG analysis showed that energy metabolism pathways, such as D-alanine metabolism and the phosphotransferase system (PTS), were enriched. We conclude that N-metabolism enzymes with higher activity and higher bacterial diversity have a significant effect on drought tolerance and nitrogen uptake in rice.https://www.frontiersin.org/articles/10.3389/fpls.2023.1120584/fullriceheading datenitrogendrought16S rRNAmetabolomics |
| spellingShingle | Changhui Sun Changhui Sun Runnan Wang Runnan Wang Guoping Tang Shuo Cai Hong Shi Fangping Liu Hengwang Xie Jinyan Zhu Jinyan Zhu Jinyan Zhu Qiangqiang Xiong Qiangqiang Xiong Qiangqiang Xiong Qiangqiang Xiong Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels Frontiers in Plant Science rice heading date nitrogen drought 16S rRNA metabolomics |
| title | Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels |
| title_full | Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels |
| title_fullStr | Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels |
| title_full_unstemmed | Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels |
| title_short | Integrated 16S and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels |
| title_sort | integrated 16s and metabolomics revealed the mechanism of drought resistance and nitrogen uptake in rice at the heading stage under different nitrogen levels |
| topic | rice heading date nitrogen drought 16S rRNA metabolomics |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1120584/full |
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