A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS
Abstract. The phenotype and functional properties of antigen-presenting cells (APCs: blood monocytes and in vitro generated macrophages/dendritic cells) were investigated in patients with pulmonary tuberculosis (TB, n = 192) with different levels of proliferative response to M. tuberculosis antigens...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/311 |
| _version_ | 1797198105495470080 |
|---|---|
| author | L. V. Sakhno Zh. M. Raspay M. A. Tikhonova S. D. Niconov O. A. Zhdanov A. A. Ostanin E. R. Chernykh |
| author_facet | L. V. Sakhno Zh. M. Raspay M. A. Tikhonova S. D. Niconov O. A. Zhdanov A. A. Ostanin E. R. Chernykh |
| author_sort | L. V. Sakhno |
| collection | DOAJ |
| description | Abstract. The phenotype and functional properties of antigen-presenting cells (APCs: blood monocytes and in vitro generated macrophages/dendritic cells) were investigated in patients with pulmonary tuberculosis (TB, n = 192) with different levels of proliferative response to M. tuberculosis antigens (PPD-responsive vs PPD - anergic patients, n = 118 and 74, respectively). A functional deficiency of all 3 types of APCs was revealed in patients with TB. I.e., a monocyte disfunction was displayed by low CD86 and HLA-DR expression, 2-fold increase of CD14+CD16+ subset, high level of FasL+ and IL-10+ cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The in vitro generated macrophages from blood monocytes challenged with GM-CSF, were characterized by shifted Th1/Th2 balance (down-regulated production of IFNγ and IL-18 combined with up-regulation of IL-6 and IL-10), and reduced allostimulatory activity in mixed lymphocyte culture. The dendritic cells were characterized by decrease of mature, activated CD25+ cells, low level of IFNγ production in conjunction with enhanced capacity to produce IL - 10 and IL-6, and profound reduction of functional (allostimulatory) activity. The APC disfunction of were most prominent in PPD-anergic patients. A possible role of APC disfunctions in disturbed antigen-specific T-cell response to M. tuberculosis is discussed. |
| first_indexed | 2024-03-08T05:51:49Z |
| format | Article |
| id | doaj.art-9a38ace2e78644b48d34fc7852cc8e93 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:54:34Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-9a38ace2e78644b48d34fc7852cc8e932024-04-22T13:07:35ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-01112-324525410.15789/1563-0625-2009-2-3-245-254308A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSISL. V. Sakhno0Zh. M. Raspay1M. A. Tikhonova2S. D. Niconov3O. A. Zhdanov4A. A. Ostanin5E. R. Chernykh6ГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскОГУЗ Новосибирская клиническая туберкулезная больница № 1, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскГУ НИИ клинической иммунологии СО РАМН, г. НовосибирскAbstract. The phenotype and functional properties of antigen-presenting cells (APCs: blood monocytes and in vitro generated macrophages/dendritic cells) were investigated in patients with pulmonary tuberculosis (TB, n = 192) with different levels of proliferative response to M. tuberculosis antigens (PPD-responsive vs PPD - anergic patients, n = 118 and 74, respectively). A functional deficiency of all 3 types of APCs was revealed in patients with TB. I.e., a monocyte disfunction was displayed by low CD86 and HLA-DR expression, 2-fold increase of CD14+CD16+ subset, high level of FasL+ and IL-10+ cells, and enhanced IL-10 and IL-6 production upon LPS-stimulation. The in vitro generated macrophages from blood monocytes challenged with GM-CSF, were characterized by shifted Th1/Th2 balance (down-regulated production of IFNγ and IL-18 combined with up-regulation of IL-6 and IL-10), and reduced allostimulatory activity in mixed lymphocyte culture. The dendritic cells were characterized by decrease of mature, activated CD25+ cells, low level of IFNγ production in conjunction with enhanced capacity to produce IL - 10 and IL-6, and profound reduction of functional (allostimulatory) activity. The APC disfunction of were most prominent in PPD-anergic patients. A possible role of APC disfunctions in disturbed antigen-specific T-cell response to M. tuberculosis is discussed.https://www.mimmun.ru/mimmun/article/view/311monocytesmacrophagesdendritic cellsиcytokinespulmonary tuberculosis |
| spellingShingle | L. V. Sakhno Zh. M. Raspay M. A. Tikhonova S. D. Niconov O. A. Zhdanov A. A. Ostanin E. R. Chernykh A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS Медицинская иммунология monocytes macrophages dendritic cellsи cytokines pulmonary tuberculosis |
| title | A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS |
| title_full | A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS |
| title_fullStr | A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS |
| title_full_unstemmed | A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS |
| title_short | A DEFICIENCY OF ANTIGEN-PRESENTING CELLS IN PATIENTS WITH PULMONARY TUBERCULOSIS |
| title_sort | deficiency of antigen presenting cells in patients with pulmonary tuberculosis |
| topic | monocytes macrophages dendritic cellsи cytokines pulmonary tuberculosis |
| url | https://www.mimmun.ru/mimmun/article/view/311 |
| work_keys_str_mv | AT lvsakhno adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT zhmraspay adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT matikhonova adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT sdniconov adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT oazhdanov adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT aaostanin adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT erchernykh adeficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT lvsakhno deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT zhmraspay deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT matikhonova deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT sdniconov deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT oazhdanov deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT aaostanin deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis AT erchernykh deficiencyofantigenpresentingcellsinpatientswithpulmonarytuberculosis |