Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China
Temperature variation affects the growth performance of fish species due to growing constraints and trade-off on physiological functions. Here we experimentally investigated several metabolic and physiological parameters in Sinilabeo rendahli, an endemic fish species found in the Yangtze River in Ch...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2023.1159161/full |
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| author | Sheng Li Sheng Li Hui Guo Chun-Ying Du Yi-Xi Tao Jing-Yun Feng Hao Xu Hao Xu Xu Pang Xu Pang Yun Li Yun Li |
| author_facet | Sheng Li Sheng Li Hui Guo Chun-Ying Du Yi-Xi Tao Jing-Yun Feng Hao Xu Hao Xu Xu Pang Xu Pang Yun Li Yun Li |
| author_sort | Sheng Li |
| collection | DOAJ |
| description | Temperature variation affects the growth performance of fish species due to growing constraints and trade-off on physiological functions. Here we experimentally investigated several metabolic and physiological parameters in Sinilabeo rendahli, an endemic fish species found in the Yangtze River in China. After a 14-day acclimation period, we measured routine metabolic rate (RMR), individual metabolic rate (MO2), temperature quotient (Q10), loss of equilibrium (LOE), and critical oxygen tension (Pcrit) at three different temperatures (15, 20, and 25°C). Moreover, we sampled the muscle tissue from juvenile S. rendahli under experimental conditions after 28 days of acclimation and performed transcriptome-RNA sequencing (RNA-seq). The Pcrit of the fish at the above acclimation temperatures were determined to be 1.07, 1.28, and 1.33 mg·L−1, respectively, and corresponded with increasing acclimation temperatures (15–25°C). RMR was positively correlated with Pcrit (r = 0.4711, P = 0.0201), negatively correlated with LOE (r = −0.4284, P = 0.0367), and significantly positively correlated with MRcrit (r = 0.8797, P < 0.001) at temperatures ranging from 15 to 25°C. In addition, a total of 4,710 differentially expressed genes (DEGs) were identified. The results of DEG analysis and KEGG clustering analysis indicated that energy metabolism played a central role in thermal stress in S. rendahli for the major upregulated genes. This was followed by autophagy, mitophagy, cardiac muscle contraction, extracellular matrix (ECM)-receptor interaction, and protein digestion and absorption. This study is significant for understanding the adaptive response of S. rendahli to thermal stress. Even more importantly, this study demonstrates that S. rendahli is more suitable for cold-water life. |
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| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-03-13T08:06:31Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-9a3c5d0978af46c883d99481edffda642023-06-01T04:38:44ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2023-06-011110.3389/fevo.2023.11591611159161Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, ChinaSheng Li0Sheng Li1Hui Guo2Chun-Ying Du3Yi-Xi Tao4Jing-Yun Feng5Hao Xu6Hao Xu7Xu Pang8Xu Pang9Yun Li10Yun Li11Fisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University, Chongqing, ChinaFisheries and Aquaculture Biotechnology Laboratory, College of Fisheries, Southwest University, Chongqing, ChinaKey Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, Southwest University, Chongqing, ChinaTemperature variation affects the growth performance of fish species due to growing constraints and trade-off on physiological functions. Here we experimentally investigated several metabolic and physiological parameters in Sinilabeo rendahli, an endemic fish species found in the Yangtze River in China. After a 14-day acclimation period, we measured routine metabolic rate (RMR), individual metabolic rate (MO2), temperature quotient (Q10), loss of equilibrium (LOE), and critical oxygen tension (Pcrit) at three different temperatures (15, 20, and 25°C). Moreover, we sampled the muscle tissue from juvenile S. rendahli under experimental conditions after 28 days of acclimation and performed transcriptome-RNA sequencing (RNA-seq). The Pcrit of the fish at the above acclimation temperatures were determined to be 1.07, 1.28, and 1.33 mg·L−1, respectively, and corresponded with increasing acclimation temperatures (15–25°C). RMR was positively correlated with Pcrit (r = 0.4711, P = 0.0201), negatively correlated with LOE (r = −0.4284, P = 0.0367), and significantly positively correlated with MRcrit (r = 0.8797, P < 0.001) at temperatures ranging from 15 to 25°C. In addition, a total of 4,710 differentially expressed genes (DEGs) were identified. The results of DEG analysis and KEGG clustering analysis indicated that energy metabolism played a central role in thermal stress in S. rendahli for the major upregulated genes. This was followed by autophagy, mitophagy, cardiac muscle contraction, extracellular matrix (ECM)-receptor interaction, and protein digestion and absorption. This study is significant for understanding the adaptive response of S. rendahli to thermal stress. Even more importantly, this study demonstrates that S. rendahli is more suitable for cold-water life.https://www.frontiersin.org/articles/10.3389/fevo.2023.1159161/fullSinilabeo rendahlitemperature acclimationcritical oxygen tension (Pcrit)routine metabolic rate (RMR)RNA-seq |
| spellingShingle | Sheng Li Sheng Li Hui Guo Chun-Ying Du Yi-Xi Tao Jing-Yun Feng Hao Xu Hao Xu Xu Pang Xu Pang Yun Li Yun Li Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China Frontiers in Ecology and Evolution Sinilabeo rendahli temperature acclimation critical oxygen tension (Pcrit) routine metabolic rate (RMR) RNA-seq |
| title | Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China |
| title_full | Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China |
| title_fullStr | Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China |
| title_full_unstemmed | Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China |
| title_short | Effect of temperature on exercise metabolism, hypoxia tolerance, and RNA-seq analysis in Sinilabeo rendahli from the Yangtze River, China |
| title_sort | effect of temperature on exercise metabolism hypoxia tolerance and rna seq analysis in sinilabeo rendahli from the yangtze river china |
| topic | Sinilabeo rendahli temperature acclimation critical oxygen tension (Pcrit) routine metabolic rate (RMR) RNA-seq |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2023.1159161/full |
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