Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China
Background Endophytic fungi influence the quality and quantity of the medicinal plant’s bioactive compounds through specific fungus-host interactions. Nevertheless, due to the paucity of information, the composition of endophytic fungal communities and the mechanism by which effective ingredients re...
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PeerJ Inc.
2021-03-01
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| Online Access: | https://peerj.com/articles/11047.pdf |
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| author | Hanli Dang Tao Zhang Zhongke Wang Guifang Li Wenqin Zhao Xinhua Lv Li Zhuang |
| author_facet | Hanli Dang Tao Zhang Zhongke Wang Guifang Li Wenqin Zhao Xinhua Lv Li Zhuang |
| author_sort | Hanli Dang |
| collection | DOAJ |
| description | Background Endophytic fungi influence the quality and quantity of the medicinal plant’s bioactive compounds through specific fungus-host interactions. Nevertheless, due to the paucity of information, the composition of endophytic fungal communities and the mechanism by which effective ingredients regulate endophytic fungal communities in roots remains unclear. Methods In this study, we collected root and soil samples (depth range: 0–20, 20–40, and 40–60 cm) of three Glycyrrhiza species (Glycyrrhiza uralensis, Glycyrrhiza inflata, and Glycyrrhiza glabra). Glycyrrhizic acid and liquiritin content were determined using high-performance liquid chromatography (HPLC), and total flavonoid content was determined using ultraviolet spectrophotometry. High-throughput sequencing technology was employed to explore the composition and diversity of the endophytic fungal community in different root segments of three Glycyrrhiza species. Furthermore, soil samples were subjected to physicochemical analyses. Results We observed that the liquiritin content was not affected by the root depth (0–20 cm, 20–40 cm, and 40–60 cm). Still, it was significantly affected by the Glycyrrhiza species (Glycyrrhiza uralensis, Glycyrrhiza inflata, Glycyrrhiza glabra) (P < 0.05). In Glycyrrhiza root, a total of eight phyla and 140 genera were annotated so far, out of which Ascomycota and Basidiomycota phyla, and the Fusarium, Paraphoma, and Helminthosporium genera were found to be significantly dominant. Spearman correlation analysis revealed that liquiritin content was accountable for the differences in the diversity of the endophytic fungal community. Furthermore, distance-based redundancy analysis (db-RDA) showed that physicochemical properties of the soil (available potassium and ammonium nitrogen) and the root factors (liquiritin and water content) were the main contributing factors for the variations in the overall structure of the endophytic fungal community. Our results showed that the effective ingredients of Glycyrrhiza root and physicochemical properties of the soil regulated the endophytic fungal community composition and medicinal licorice diversity. |
| first_indexed | 2024-03-09T07:53:46Z |
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| language | English |
| last_indexed | 2024-03-09T07:53:46Z |
| publishDate | 2021-03-01 |
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| spelling | doaj.art-9a3db78398b046f7894c03fe15f165662023-12-03T01:22:07ZengPeerJ Inc.PeerJ2167-83592021-03-019e1104710.7717/peerj.11047Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, ChinaHanli Dang0Tao Zhang1Zhongke Wang2Guifang Li3Wenqin Zhao4Xinhua Lv5Li Zhuang6College of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Life Sciences, Shihezi University, Shihezi, Xinjiang, ChinaBackground Endophytic fungi influence the quality and quantity of the medicinal plant’s bioactive compounds through specific fungus-host interactions. Nevertheless, due to the paucity of information, the composition of endophytic fungal communities and the mechanism by which effective ingredients regulate endophytic fungal communities in roots remains unclear. Methods In this study, we collected root and soil samples (depth range: 0–20, 20–40, and 40–60 cm) of three Glycyrrhiza species (Glycyrrhiza uralensis, Glycyrrhiza inflata, and Glycyrrhiza glabra). Glycyrrhizic acid and liquiritin content were determined using high-performance liquid chromatography (HPLC), and total flavonoid content was determined using ultraviolet spectrophotometry. High-throughput sequencing technology was employed to explore the composition and diversity of the endophytic fungal community in different root segments of three Glycyrrhiza species. Furthermore, soil samples were subjected to physicochemical analyses. Results We observed that the liquiritin content was not affected by the root depth (0–20 cm, 20–40 cm, and 40–60 cm). Still, it was significantly affected by the Glycyrrhiza species (Glycyrrhiza uralensis, Glycyrrhiza inflata, Glycyrrhiza glabra) (P < 0.05). In Glycyrrhiza root, a total of eight phyla and 140 genera were annotated so far, out of which Ascomycota and Basidiomycota phyla, and the Fusarium, Paraphoma, and Helminthosporium genera were found to be significantly dominant. Spearman correlation analysis revealed that liquiritin content was accountable for the differences in the diversity of the endophytic fungal community. Furthermore, distance-based redundancy analysis (db-RDA) showed that physicochemical properties of the soil (available potassium and ammonium nitrogen) and the root factors (liquiritin and water content) were the main contributing factors for the variations in the overall structure of the endophytic fungal community. Our results showed that the effective ingredients of Glycyrrhiza root and physicochemical properties of the soil regulated the endophytic fungal community composition and medicinal licorice diversity.https://peerj.com/articles/11047.pdfEffective ingredientsEndophytic fungal communityGlycyrrhizaSoil physicochemicalRoot depth |
| spellingShingle | Hanli Dang Tao Zhang Zhongke Wang Guifang Li Wenqin Zhao Xinhua Lv Li Zhuang Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China PeerJ Effective ingredients Endophytic fungal community Glycyrrhiza Soil physicochemical Root depth |
| title | Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China |
| title_full | Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China |
| title_fullStr | Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China |
| title_full_unstemmed | Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China |
| title_short | Differences in the endophytic fungal community and effective ingredients in root of three Glycyrrhiza species in Xinjiang, China |
| title_sort | differences in the endophytic fungal community and effective ingredients in root of three glycyrrhiza species in xinjiang china |
| topic | Effective ingredients Endophytic fungal community Glycyrrhiza Soil physicochemical Root depth |
| url | https://peerj.com/articles/11047.pdf |
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