First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua
Abstract Background Although many vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas and potential zoonoses, there is little information on these conditions in Central America. Methods Seven qPCRs for vector-borne pathogens were performed on a Roche Light...
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| Format: | Article |
| Language: | English |
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BMC
2014-03-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/1756-3305-7-126 |
| _version_ | 1797811699778060288 |
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| author | Lanjing Wei Patrick Kelly Kate Ackerson Jilei Zhang Heba S El-Mahallawy Bernhard Kaltenboeck Chengming Wang |
| author_facet | Lanjing Wei Patrick Kelly Kate Ackerson Jilei Zhang Heba S El-Mahallawy Bernhard Kaltenboeck Chengming Wang |
| author_sort | Lanjing Wei |
| collection | DOAJ |
| description | Abstract Background Although many vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas and potential zoonoses, there is little information on these conditions in Central America. Methods Seven qPCRs for vector-borne pathogens were performed on a Roche LightCycler PCR Instrument to investigate their prevalence in a convenience sample of whole blood samples from apparently healthy dogs in Nicaragua. Also, a qPCR targeting the canine hydroxymethylbilane synthase (HMBS) gene was used as an endogenous internal control and verified the quality and quantity of DNA in the samples was appropriate for the study. Results We found DNA of Rickettsia felis (5%), Babesia spp. (26%), Hepatozoon canis (51%), Anaplasma platys (13%) and Ehrlichia canis (56%) in the 39 dogs studied. The qPCRs for Coxiella burnetii and Dirofilaria immitis were negative. Of the 30 (80%) dogs that were positive by qPCR, 12 (31%) were positive for one agent, 11 (28%) for two, 3 (8%) for three, and 4 (10%) for four agents. Conclusions This is the first report of B. gibsoni in dogs from Central America and the first recording of vector-borne agents in dogs from Nicaragua. Dogs in Nicaragua are commonly infected with a variety of vector-borne pathogens, some of which may also infect people. |
| first_indexed | 2024-03-13T07:27:36Z |
| format | Article |
| id | doaj.art-9a3fa5d8753e4f86b748493e5ea55117 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:36Z |
| publishDate | 2014-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-9a3fa5d8753e4f86b748493e5ea551172023-06-04T11:17:43ZengBMCParasites & Vectors1756-33052014-03-01711610.1186/1756-3305-7-126First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from NicaraguaLanjing Wei0Patrick Kelly1Kate Ackerson2Jilei Zhang3Heba S El-Mahallawy4Bernhard Kaltenboeck5Chengming Wang6Yangzhou University College of Veterinary MedicineRoss University School of Veterinary MedicineRoss University School of Veterinary MedicineYangzhou University College of Veterinary MedicineYangzhou University College of Veterinary MedicineAuburn University College of Veterinary MedicineYangzhou University College of Veterinary MedicineAbstract Background Although many vector-borne diseases are important causes of morbidity and mortality in dogs in tropical areas and potential zoonoses, there is little information on these conditions in Central America. Methods Seven qPCRs for vector-borne pathogens were performed on a Roche LightCycler PCR Instrument to investigate their prevalence in a convenience sample of whole blood samples from apparently healthy dogs in Nicaragua. Also, a qPCR targeting the canine hydroxymethylbilane synthase (HMBS) gene was used as an endogenous internal control and verified the quality and quantity of DNA in the samples was appropriate for the study. Results We found DNA of Rickettsia felis (5%), Babesia spp. (26%), Hepatozoon canis (51%), Anaplasma platys (13%) and Ehrlichia canis (56%) in the 39 dogs studied. The qPCRs for Coxiella burnetii and Dirofilaria immitis were negative. Of the 30 (80%) dogs that were positive by qPCR, 12 (31%) were positive for one agent, 11 (28%) for two, 3 (8%) for three, and 4 (10%) for four agents. Conclusions This is the first report of B. gibsoni in dogs from Central America and the first recording of vector-borne agents in dogs from Nicaragua. Dogs in Nicaragua are commonly infected with a variety of vector-borne pathogens, some of which may also infect people.https://doi.org/10.1186/1756-3305-7-126NicaraguaRickettsia felisBabesia gibsoni/vogeliHepatozoon canisAnaplasma platysEhrlichia canis |
| spellingShingle | Lanjing Wei Patrick Kelly Kate Ackerson Jilei Zhang Heba S El-Mahallawy Bernhard Kaltenboeck Chengming Wang First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua Parasites & Vectors Nicaragua Rickettsia felis Babesia gibsoni/vogeli Hepatozoon canis Anaplasma platys Ehrlichia canis |
| title | First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua |
| title_full | First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua |
| title_fullStr | First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua |
| title_full_unstemmed | First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua |
| title_short | First report of Babesia gibsoni in Central America and survey for vector-borne infections in dogs from Nicaragua |
| title_sort | first report of babesia gibsoni in central america and survey for vector borne infections in dogs from nicaragua |
| topic | Nicaragua Rickettsia felis Babesia gibsoni/vogeli Hepatozoon canis Anaplasma platys Ehrlichia canis |
| url | https://doi.org/10.1186/1756-3305-7-126 |
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