Role of growth rate on the orientational alignment of Escherichia coli in a slit
We present experimental data on the nematic alignment of Escherichia coli bacteria confined in a slit, with an emphasis on the effect of growth rate and corresponding changes in cell aspect ratio. Global alignment with the channel walls arises from the combination of local nematic ordering of nearby...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2017-01-01
|
| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.170463 |
| _version_ | 1819202790453411840 |
|---|---|
| author | Julian Sheats Bianca Sclavi Marco Cosentino Lagomarsino Pietro Cicuta Kevin D. Dorfman |
| author_facet | Julian Sheats Bianca Sclavi Marco Cosentino Lagomarsino Pietro Cicuta Kevin D. Dorfman |
| author_sort | Julian Sheats |
| collection | DOAJ |
| description | We present experimental data on the nematic alignment of Escherichia coli bacteria confined in a slit, with an emphasis on the effect of growth rate and corresponding changes in cell aspect ratio. Global alignment with the channel walls arises from the combination of local nematic ordering of nearby cells, induced by cell division and the elongated shape of the cells, and the preferential orientation of cells proximate to the side walls of the slit. Decreasing the growth rate leads to a decrease in alignment with the walls, which is attributed primarily to effects of changing cell aspect ratio rather than changes in the variance in cell area. Decreasing confinement also reduces the degree of alignment by a similar amount as a decrease in the growth rate, but the distribution of the degree of alignment differs. The onset of alignment with the channel walls is coincident with the slits reaching their steady-state occupancy and connected to the re-orientation of locally aligned regions with respect to the walls during density fluctuations. |
| first_indexed | 2024-12-23T04:09:37Z |
| format | Article |
| id | doaj.art-9a422cee4d6a4c08bccc015778a11d9b |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-23T04:09:37Z |
| publishDate | 2017-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-9a422cee4d6a4c08bccc015778a11d9b2022-12-21T18:00:33ZengThe Royal SocietyRoyal Society Open Science2054-57032017-01-014610.1098/rsos.170463170463Role of growth rate on the orientational alignment of Escherichia coli in a slitJulian SheatsBianca SclaviMarco Cosentino LagomarsinoPietro CicutaKevin D. DorfmanWe present experimental data on the nematic alignment of Escherichia coli bacteria confined in a slit, with an emphasis on the effect of growth rate and corresponding changes in cell aspect ratio. Global alignment with the channel walls arises from the combination of local nematic ordering of nearby cells, induced by cell division and the elongated shape of the cells, and the preferential orientation of cells proximate to the side walls of the slit. Decreasing the growth rate leads to a decrease in alignment with the walls, which is attributed primarily to effects of changing cell aspect ratio rather than changes in the variance in cell area. Decreasing confinement also reduces the degree of alignment by a similar amount as a decrease in the growth rate, but the distribution of the degree of alignment differs. The onset of alignment with the channel walls is coincident with the slits reaching their steady-state occupancy and connected to the re-orientation of locally aligned regions with respect to the walls during density fluctuations.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.170463microchemostatbacteria colonynematic alignmentmicrofluidics |
| spellingShingle | Julian Sheats Bianca Sclavi Marco Cosentino Lagomarsino Pietro Cicuta Kevin D. Dorfman Role of growth rate on the orientational alignment of Escherichia coli in a slit Royal Society Open Science microchemostat bacteria colony nematic alignment microfluidics |
| title | Role of growth rate on the orientational alignment of Escherichia coli in a slit |
| title_full | Role of growth rate on the orientational alignment of Escherichia coli in a slit |
| title_fullStr | Role of growth rate on the orientational alignment of Escherichia coli in a slit |
| title_full_unstemmed | Role of growth rate on the orientational alignment of Escherichia coli in a slit |
| title_short | Role of growth rate on the orientational alignment of Escherichia coli in a slit |
| title_sort | role of growth rate on the orientational alignment of escherichia coli in a slit |
| topic | microchemostat bacteria colony nematic alignment microfluidics |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.170463 |
| work_keys_str_mv | AT juliansheats roleofgrowthrateontheorientationalalignmentofescherichiacoliinaslit AT biancasclavi roleofgrowthrateontheorientationalalignmentofescherichiacoliinaslit AT marcocosentinolagomarsino roleofgrowthrateontheorientationalalignmentofescherichiacoliinaslit AT pietrocicuta roleofgrowthrateontheorientationalalignmentofescherichiacoliinaslit AT kevinddorfman roleofgrowthrateontheorientationalalignmentofescherichiacoliinaslit |