Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil
Bacterial activity, mainly Pseudomonas spp. plays a vital role in the fruiting process of white button mushroom, hence a rapid procedure to identify these bacteria is crucial. In the current study, the validity of commercial identification system, Analytical profile index API 20E to identify Pseudom...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2020-06-01
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| Series: | Notulae Scientia Biologicae |
| Subjects: | |
| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10628 |
| _version_ | 1818000815370010624 |
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| author | Anwar O. MOHAMMAD Abdulkareem E.S. ALKURTANY Abdullah A. HASSAN |
| author_facet | Anwar O. MOHAMMAD Abdulkareem E.S. ALKURTANY Abdullah A. HASSAN |
| author_sort | Anwar O. MOHAMMAD |
| collection | DOAJ |
| description | Bacterial activity, mainly Pseudomonas spp. plays a vital role in the fruiting process of white button mushroom, hence a rapid procedure to identify these bacteria is crucial. In the current study, the validity of commercial identification system, Analytical profile index API 20E to identify Pseudomonas isolates from mushroom casing soil were assessed. Using API strips fifty bacterial isolates from a selective medium (King B medium) were examined, all isolates were belonged to the genus Pseudomonas according to API 20E identification systems. However, only 74% of Pseudomonas bacteria were identified to species level. The molecular identification using 16S rRNA gene was used as a reference tool to identify bacteria at the species level. The results show that the accuracy of the system to classify florescent Pseudomonas to species level was 60%. This was species dependant, and the system accuracy were 100%, 87.5%, 81.3% and 63% in identifying P. aeruginosa, P. putida, P. fluorescens and P. tolaasii respectively. Our finding indicates that although the classification of the Pseudomonas genus with API 20E system is useful, but it is not enough to distinguish these bacteria to species level, genomic studies are necessary to confirm the exact taxonomic position of Pseudomonas spp. |
| first_indexed | 2024-04-14T03:26:28Z |
| format | Article |
| id | doaj.art-9a43606281704af5ac413f7ff9723541 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T03:26:28Z |
| publishDate | 2020-06-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-9a43606281704af5ac413f7ff97235412022-12-22T02:15:10ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642020-06-0112210.15835/nsb12210628Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soilAnwar O. MOHAMMAD0Abdulkareem E.S. ALKURTANY1Abdullah A. HASSAN2University of Sulaimani, Natural Resources Department, College of Agricultural Engineering Sciences, Al-SulaymaniyahTikrit University, Department of Soil and Water Science, College of Agriculture, TikritTikrit University, Department of Plant Protection, College of Agriculture, TikritBacterial activity, mainly Pseudomonas spp. plays a vital role in the fruiting process of white button mushroom, hence a rapid procedure to identify these bacteria is crucial. In the current study, the validity of commercial identification system, Analytical profile index API 20E to identify Pseudomonas isolates from mushroom casing soil were assessed. Using API strips fifty bacterial isolates from a selective medium (King B medium) were examined, all isolates were belonged to the genus Pseudomonas according to API 20E identification systems. However, only 74% of Pseudomonas bacteria were identified to species level. The molecular identification using 16S rRNA gene was used as a reference tool to identify bacteria at the species level. The results show that the accuracy of the system to classify florescent Pseudomonas to species level was 60%. This was species dependant, and the system accuracy were 100%, 87.5%, 81.3% and 63% in identifying P. aeruginosa, P. putida, P. fluorescens and P. tolaasii respectively. Our finding indicates that although the classification of the Pseudomonas genus with API 20E system is useful, but it is not enough to distinguish these bacteria to species level, genomic studies are necessary to confirm the exact taxonomic position of Pseudomonas spp.https://www.notulaebiologicae.ro/index.php/nsb/article/view/10628bacteria; biochemical tests; classification; fluorescent Pseudomonas; PCR |
| spellingShingle | Anwar O. MOHAMMAD Abdulkareem E.S. ALKURTANY Abdullah A. HASSAN Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil Notulae Scientia Biologicae bacteria; biochemical tests; classification; fluorescent Pseudomonas; PCR |
| title | Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil |
| title_full | Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil |
| title_fullStr | Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil |
| title_full_unstemmed | Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil |
| title_short | Evaluation of API 20E system in fluorescent Pseudomonas identification from button mushroom Agaricus bisporus cultivation casing soil |
| title_sort | evaluation of api 20e system in fluorescent pseudomonas identification from button mushroom agaricus bisporus cultivation casing soil |
| topic | bacteria; biochemical tests; classification; fluorescent Pseudomonas; PCR |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10628 |
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