Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass
Abstract Background Breeding a mare until she is not fertile or even until her death is common in equine industry but the fertility decreases as the mare age increases. Embryo loss due to reduced embryo quality is partly accountable for this observation. Here, the effect of mare’s age on blastocysts...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-08593-7 |
| _version_ | 1811242299015823360 |
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| author | Emilie Derisoud Luc Jouneau Cédric Dubois Catherine Archilla Yan Jaszczyszyn Rachel Legendre Nathalie Daniel Nathalie Peynot Michèle Dahirel Juliette Auclair-Ronzaud Laurence Wimel Véronique Duranthon Pascale Chavatte-Palmer |
| author_facet | Emilie Derisoud Luc Jouneau Cédric Dubois Catherine Archilla Yan Jaszczyszyn Rachel Legendre Nathalie Daniel Nathalie Peynot Michèle Dahirel Juliette Auclair-Ronzaud Laurence Wimel Véronique Duranthon Pascale Chavatte-Palmer |
| author_sort | Emilie Derisoud |
| collection | DOAJ |
| description | Abstract Background Breeding a mare until she is not fertile or even until her death is common in equine industry but the fertility decreases as the mare age increases. Embryo loss due to reduced embryo quality is partly accountable for this observation. Here, the effect of mare’s age on blastocysts’ gene expression was explored. Day 8 post-ovulation embryos were collected from multiparous young (YM, 6-year-old, N = 5) and older (OM, > 10-year-old, N = 6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure or inner cell mass (ICM) enriched trophoblast, obtained by embryo bisection, were RNA sequenced. Deconvolution algorithm was used to discriminate gene expression in the ICM from that in the trophoblast. Differential expression was analyzed with embryo sex and diameter as cofactors. Functional annotation and classification of differentially expressed genes and gene set enrichment analysis were also performed. Results Maternal aging did not affect embryo recovery rate, embryo diameter nor total RNA quantity. In both compartments, the expression of genes involved in mitochondria and protein metabolism were disturbed by maternal age, although more genes were affected in the ICM. Mitosis, signaling and adhesion pathways and embryo development were decreased in the ICM of embryos from old mares. In trophoblast, ion movement pathways were affected. Conclusions This is the first study showing that maternal age affects gene expression in the equine blastocyst, demonstrating significant effects as early as 10 years of age. These perturbations may affect further embryo development and contribute to decreased fertility due to aging. |
| first_indexed | 2024-04-12T13:49:39Z |
| format | Article |
| id | doaj.art-9a463d4d23f44205b3ab620dd0c4de5b |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T13:49:39Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-9a463d4d23f44205b3ab620dd0c4de5b2022-12-22T03:30:35ZengBMCBMC Genomics1471-21642022-06-0123112210.1186/s12864-022-08593-7Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell massEmilie Derisoud0Luc Jouneau1Cédric Dubois2Catherine Archilla3Yan Jaszczyszyn4Rachel Legendre5Nathalie Daniel6Nathalie Peynot7Michèle Dahirel8Juliette Auclair-Ronzaud9Laurence Wimel10Véronique Duranthon11Pascale Chavatte-Palmer12Université Paris-Saclay, UVSQ, INRAE, BREEDUniversité Paris-Saclay, UVSQ, INRAE, BREEDIFCE, Plateau technique de ChamberetUniversité Paris-Saclay, UVSQ, INRAE, BREEDInstitute for Integrative Biology of the Cell (I2BC), UMR 9198 CNRS, CEA, Paris-Sud University F-91198Institut Pasteur—Bioinformatics and Biostatistics Hub—Department of Computational BiologyUniversité Paris-Saclay, UVSQ, INRAE, BREEDUniversité Paris-Saclay, UVSQ, INRAE, BREEDUniversité Paris-Saclay, UVSQ, INRAE, BREEDIFCE, Plateau technique de ChamberetIFCE, Plateau technique de ChamberetUniversité Paris-Saclay, UVSQ, INRAE, BREEDUniversité Paris-Saclay, UVSQ, INRAE, BREEDAbstract Background Breeding a mare until she is not fertile or even until her death is common in equine industry but the fertility decreases as the mare age increases. Embryo loss due to reduced embryo quality is partly accountable for this observation. Here, the effect of mare’s age on blastocysts’ gene expression was explored. Day 8 post-ovulation embryos were collected from multiparous young (YM, 6-year-old, N = 5) and older (OM, > 10-year-old, N = 6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure or inner cell mass (ICM) enriched trophoblast, obtained by embryo bisection, were RNA sequenced. Deconvolution algorithm was used to discriminate gene expression in the ICM from that in the trophoblast. Differential expression was analyzed with embryo sex and diameter as cofactors. Functional annotation and classification of differentially expressed genes and gene set enrichment analysis were also performed. Results Maternal aging did not affect embryo recovery rate, embryo diameter nor total RNA quantity. In both compartments, the expression of genes involved in mitochondria and protein metabolism were disturbed by maternal age, although more genes were affected in the ICM. Mitosis, signaling and adhesion pathways and embryo development were decreased in the ICM of embryos from old mares. In trophoblast, ion movement pathways were affected. Conclusions This is the first study showing that maternal age affects gene expression in the equine blastocyst, demonstrating significant effects as early as 10 years of age. These perturbations may affect further embryo development and contribute to decreased fertility due to aging.https://doi.org/10.1186/s12864-022-08593-7HorseBlastocystDeconvolutionRNA-sequencingMare |
| spellingShingle | Emilie Derisoud Luc Jouneau Cédric Dubois Catherine Archilla Yan Jaszczyszyn Rachel Legendre Nathalie Daniel Nathalie Peynot Michèle Dahirel Juliette Auclair-Ronzaud Laurence Wimel Véronique Duranthon Pascale Chavatte-Palmer Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass BMC Genomics Horse Blastocyst Deconvolution RNA-sequencing Mare |
| title | Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass |
| title_full | Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass |
| title_fullStr | Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass |
| title_full_unstemmed | Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass |
| title_short | Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass |
| title_sort | maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass |
| topic | Horse Blastocyst Deconvolution RNA-sequencing Mare |
| url | https://doi.org/10.1186/s12864-022-08593-7 |
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