Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system
<p>Abstract</p> <p>Background</p> <p>The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids between pretreatment and enzy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-07-01
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| Series: | Biotechnology for Biofuels |
| Online Access: | http://www.biotechnologyforbiofuels.com/content/4/1/19 |
| _version_ | 1818534529784086528 |
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| author | DeMartini Jaclyn D Brethauer Simone Studer Michael H McKenzie Heather L Wyman Charles E |
| author_facet | DeMartini Jaclyn D Brethauer Simone Studer Michael H McKenzie Heather L Wyman Charles E |
| author_sort | DeMartini Jaclyn D |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids between pretreatment and enzymatic hydrolysis used in conventional analyses is slow, labor-intensive and very difficult to automate, a streamlined approach we term 'co-hydrolysis' was developed. In this method, the solids and liquid in the pretreated biomass slurry are not separated, but instead hydrolysis is performed by adding enzymes to the whole pretreated slurry. The effects of pretreatment method, severity and solids loading on co-hydrolysis performance were investigated.</p> <p>Results</p> <p>For hydrothermal pretreatment at solids concentrations of 0.5 to 2%, high enzyme protein loadings of about 100 mg/g of substrate (glucan plus xylan) in the original poplar wood achieved glucose and xylose yields for co-hydrolysis that were comparable with those for washed solids. In addition, although poplar wood sugar yields from co-hydrolysis at 2% solids concentrations fell short of those from hydrolysis of washed solids after dilute sulfuric acid pretreatment even at high enzyme loadings, pretreatment at 0.5% solids concentrations resulted in similar yields for all but the lowest enzyme loading.</p> <p>Conclusions</p> <p>Overall, the influence of severity on susceptibility of pretreated substrates to enzymatic hydrolysis was clearly discernable, showing co-hydrolysis to be a viable approach for identifying plant-pretreatment-enzyme combinations with substantial advantages for sugar production.</p> |
| first_indexed | 2024-12-11T18:12:50Z |
| format | Article |
| id | doaj.art-9a486efdd16041099975a852fd9bea90 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-11T18:12:50Z |
| publishDate | 2011-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-9a486efdd16041099975a852fd9bea902022-12-22T00:55:32ZengBMCBiotechnology for Biofuels1754-68342011-07-01411910.1186/1754-6834-4-19Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment systemDeMartini Jaclyn DBrethauer SimoneStuder Michael HMcKenzie Heather LWyman Charles E<p>Abstract</p> <p>Background</p> <p>The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids between pretreatment and enzymatic hydrolysis used in conventional analyses is slow, labor-intensive and very difficult to automate, a streamlined approach we term 'co-hydrolysis' was developed. In this method, the solids and liquid in the pretreated biomass slurry are not separated, but instead hydrolysis is performed by adding enzymes to the whole pretreated slurry. The effects of pretreatment method, severity and solids loading on co-hydrolysis performance were investigated.</p> <p>Results</p> <p>For hydrothermal pretreatment at solids concentrations of 0.5 to 2%, high enzyme protein loadings of about 100 mg/g of substrate (glucan plus xylan) in the original poplar wood achieved glucose and xylose yields for co-hydrolysis that were comparable with those for washed solids. In addition, although poplar wood sugar yields from co-hydrolysis at 2% solids concentrations fell short of those from hydrolysis of washed solids after dilute sulfuric acid pretreatment even at high enzyme loadings, pretreatment at 0.5% solids concentrations resulted in similar yields for all but the lowest enzyme loading.</p> <p>Conclusions</p> <p>Overall, the influence of severity on susceptibility of pretreated substrates to enzymatic hydrolysis was clearly discernable, showing co-hydrolysis to be a viable approach for identifying plant-pretreatment-enzyme combinations with substantial advantages for sugar production.</p>http://www.biotechnologyforbiofuels.com/content/4/1/19 |
| spellingShingle | DeMartini Jaclyn D Brethauer Simone Studer Michael H McKenzie Heather L Wyman Charles E Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system Biotechnology for Biofuels |
| title | Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system |
| title_full | Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system |
| title_fullStr | Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system |
| title_full_unstemmed | Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system |
| title_short | Co-hydrolysis of hydrothermal and dilute acid pretreated <it>populus </it>slurries to support development of a high-throughput pretreatment system |
| title_sort | co hydrolysis of hydrothermal and dilute acid pretreated it populus it slurries to support development of a high throughput pretreatment system |
| url | http://www.biotechnologyforbiofuels.com/content/4/1/19 |
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