Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development
Radial glial cells are a distinct non-neuronal cell type that, during development, span the entire width of the brain walls of the ventricular system. They play a central role in the origin and placement of neurons, since their processes form structural scaffolds that guide and facilitate neuronal m...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/24/13509 |
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| author | Elizabeth Pereida-Jaramillo Gabriela B. Gómez-González Angeles Edith Espino-Saldaña Ataúlfo Martínez-Torres |
| author_facet | Elizabeth Pereida-Jaramillo Gabriela B. Gómez-González Angeles Edith Espino-Saldaña Ataúlfo Martínez-Torres |
| author_sort | Elizabeth Pereida-Jaramillo |
| collection | DOAJ |
| description | Radial glial cells are a distinct non-neuronal cell type that, during development, span the entire width of the brain walls of the ventricular system. They play a central role in the origin and placement of neurons, since their processes form structural scaffolds that guide and facilitate neuronal migration. Furthermore, glutamatergic signaling in the radial glia of the adult cerebellum (i.e., Bergmann glia), is crucial for precise motor coordination. Radial glial cells exhibit spontaneous calcium activity and functional coupling spread calcium waves. However, the origin of calcium activity in relation to the ontogeny of cerebellar radial glia has not been widely explored, and many questions remain unanswered regarding the role of radial glia in brain development in health and disease. In this study we used a combination of whole mount immunofluorescence and calcium imaging in transgenic (gfap-GCaMP6s) zebrafish to determine how development of calcium activity is related to morphological changes of the cerebellum. We found that the morphological changes in cerebellar radial glia are quite dynamic; the cells are remarkably larger and more elaborate in their soma size, process length and numbers after 7 days post fertilization. Spontaneous calcium events were scarce during the first 3 days of development and calcium waves appeared on day 5, which is associated with the onset of more complex morphologies of radial glia. Blockage of gap junction coupling inhibited the propagation of calcium waves, but not basal local calcium activity. This work establishes crucial clues in radial glia organization, morphology and calcium signaling during development and provides insight into its role in complex behavioral paradigms. |
| first_indexed | 2024-03-10T03:55:45Z |
| format | Article |
| id | doaj.art-9a487fe2868f4d029d5abd1192479584 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:55:45Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9a487fe2868f4d029d5abd11924795842023-11-23T08:47:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241350910.3390/ijms222413509Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish DevelopmentElizabeth Pereida-Jaramillo0Gabriela B. Gómez-González1Angeles Edith Espino-Saldaña2Ataúlfo Martínez-Torres3Laboratorio de Neurobiología Molecular y Celular, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México. Campus UNAM Juriquilla, Queretaro CP76230, MexicoLaboratorio de Neurobiología Molecular y Celular, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México. Campus UNAM Juriquilla, Queretaro CP76230, MexicoLaboratorio de Neurobiología Molecular y Celular, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México. Campus UNAM Juriquilla, Queretaro CP76230, MexicoLaboratorio de Neurobiología Molecular y Celular, Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México. Campus UNAM Juriquilla, Queretaro CP76230, MexicoRadial glial cells are a distinct non-neuronal cell type that, during development, span the entire width of the brain walls of the ventricular system. They play a central role in the origin and placement of neurons, since their processes form structural scaffolds that guide and facilitate neuronal migration. Furthermore, glutamatergic signaling in the radial glia of the adult cerebellum (i.e., Bergmann glia), is crucial for precise motor coordination. Radial glial cells exhibit spontaneous calcium activity and functional coupling spread calcium waves. However, the origin of calcium activity in relation to the ontogeny of cerebellar radial glia has not been widely explored, and many questions remain unanswered regarding the role of radial glia in brain development in health and disease. In this study we used a combination of whole mount immunofluorescence and calcium imaging in transgenic (gfap-GCaMP6s) zebrafish to determine how development of calcium activity is related to morphological changes of the cerebellum. We found that the morphological changes in cerebellar radial glia are quite dynamic; the cells are remarkably larger and more elaborate in their soma size, process length and numbers after 7 days post fertilization. Spontaneous calcium events were scarce during the first 3 days of development and calcium waves appeared on day 5, which is associated with the onset of more complex morphologies of radial glia. Blockage of gap junction coupling inhibited the propagation of calcium waves, but not basal local calcium activity. This work establishes crucial clues in radial glia organization, morphology and calcium signaling during development and provides insight into its role in complex behavioral paradigms.https://www.mdpi.com/1422-0067/22/24/13509calcium wavescerebellumGECIGFAPTol2 |
| spellingShingle | Elizabeth Pereida-Jaramillo Gabriela B. Gómez-González Angeles Edith Espino-Saldaña Ataúlfo Martínez-Torres Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development International Journal of Molecular Sciences calcium waves cerebellum GECI GFAP Tol2 |
| title | Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development |
| title_full | Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development |
| title_fullStr | Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development |
| title_full_unstemmed | Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development |
| title_short | Calcium Signaling in the Cerebellar Radial Glia and Its Association with Morphological Changes during Zebrafish Development |
| title_sort | calcium signaling in the cerebellar radial glia and its association with morphological changes during zebrafish development |
| topic | calcium waves cerebellum GECI GFAP Tol2 |
| url | https://www.mdpi.com/1422-0067/22/24/13509 |
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