Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts
Background: Small-diameter (<6 mm) artificial vascular grafts (AVGs) are urgently required in vessel reconstructive surgery but constrained by suboptimal hemocompatibility and the complexity of anastomotic procedures. This study introduces coaxial electrospinning and magnetic anastomosis tech...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1331078/full |
| _version_ | 1797347898211434496 |
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| author | Peng Liu Peng Liu Xin Liu Lifei Yang Yerong Qian Qiang Lu Aihua Shi Shasha Wei Xufeng Zhang Yi Lv Yi Lv Yi Lv Junxi Xiang |
| author_facet | Peng Liu Peng Liu Xin Liu Lifei Yang Yerong Qian Qiang Lu Aihua Shi Shasha Wei Xufeng Zhang Yi Lv Yi Lv Yi Lv Junxi Xiang |
| author_sort | Peng Liu |
| collection | DOAJ |
| description | Background: Small-diameter (<6 mm) artificial vascular grafts (AVGs) are urgently required in vessel reconstructive surgery but constrained by suboptimal hemocompatibility and the complexity of anastomotic procedures. This study introduces coaxial electrospinning and magnetic anastomosis techniques to improve graft performance.Methods: Bilayer poly(lactide-co-caprolactone) (PLCL) grafts were fabricated by coaxial electrospinning to encapsulate heparin in the inner layer for anticoagulation. Magnetic rings were embedded at both ends of the nanofiber conduit to construct a magnetic anastomosis small-diameter AVG. Material properties were characterized by micromorphology, fourier transform infrared (FTIR) spectra, mechanical tests, in vitro heparin release and hemocompatibility. In vivo performance was evaluated in a rabbit model of inferior vena cava replacement.Results: Coaxial electrospinning produced PLCL/heparin grafts with sustained heparin release, lower platelet adhesion, prolonged clotting times, higher Young’s modulus and tensile strength versus PLCL grafts. Magnetic anastomosis was significantly faster than suturing (3.65 ± 0.83 vs. 20.32 ± 3.45 min, p < 0.001) and with higher success rate (100% vs. 80%). Furthermore, magnetic AVG had higher short-term patency (2 days: 100% vs. 60%; 7 days: 40% vs. 0%) but similar long-term occlusion as sutured grafts.Conclusion: Coaxial electrospinning improved hemocompatibility and magnetic anastomosis enhanced implantability of small-diameter AVG. Short-term patency was excellent, but further optimization of anticoagulation is needed for long-term patency. This combinatorial approach holds promise for vascular graft engineering. |
| first_indexed | 2024-03-08T11:55:35Z |
| format | Article |
| id | doaj.art-9a51e9d7ff6a48d6b0351ac3d37f6e54 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T11:55:35Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-9a51e9d7ff6a48d6b0351ac3d37f6e542024-01-24T04:43:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-01-011210.3389/fbioe.2024.13310781331078Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular graftsPeng Liu0Peng Liu1Xin Liu2Lifei Yang3Yerong Qian4Qiang Lu5Aihua Shi6Shasha Wei7Xufeng Zhang8Yi Lv9Yi Lv10Yi Lv11Junxi Xiang12Center for Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Graduate School, Xi’an Medical University, Xi’an, Shaanxi, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Geriatric Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaCenter for Regenerative and Reconstructive Medicine, Med-X Institute, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaNational Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, ChinaBackground: Small-diameter (<6 mm) artificial vascular grafts (AVGs) are urgently required in vessel reconstructive surgery but constrained by suboptimal hemocompatibility and the complexity of anastomotic procedures. This study introduces coaxial electrospinning and magnetic anastomosis techniques to improve graft performance.Methods: Bilayer poly(lactide-co-caprolactone) (PLCL) grafts were fabricated by coaxial electrospinning to encapsulate heparin in the inner layer for anticoagulation. Magnetic rings were embedded at both ends of the nanofiber conduit to construct a magnetic anastomosis small-diameter AVG. Material properties were characterized by micromorphology, fourier transform infrared (FTIR) spectra, mechanical tests, in vitro heparin release and hemocompatibility. In vivo performance was evaluated in a rabbit model of inferior vena cava replacement.Results: Coaxial electrospinning produced PLCL/heparin grafts with sustained heparin release, lower platelet adhesion, prolonged clotting times, higher Young’s modulus and tensile strength versus PLCL grafts. Magnetic anastomosis was significantly faster than suturing (3.65 ± 0.83 vs. 20.32 ± 3.45 min, p < 0.001) and with higher success rate (100% vs. 80%). Furthermore, magnetic AVG had higher short-term patency (2 days: 100% vs. 60%; 7 days: 40% vs. 0%) but similar long-term occlusion as sutured grafts.Conclusion: Coaxial electrospinning improved hemocompatibility and magnetic anastomosis enhanced implantability of small-diameter AVG. Short-term patency was excellent, but further optimization of anticoagulation is needed for long-term patency. This combinatorial approach holds promise for vascular graft engineering.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1331078/fullcoaxial electrospinningmagnetic anastomosissmall-diameter vascular graftshemocompatibilitysutureless |
| spellingShingle | Peng Liu Peng Liu Xin Liu Lifei Yang Yerong Qian Qiang Lu Aihua Shi Shasha Wei Xufeng Zhang Yi Lv Yi Lv Yi Lv Junxi Xiang Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts Frontiers in Bioengineering and Biotechnology coaxial electrospinning magnetic anastomosis small-diameter vascular grafts hemocompatibility sutureless |
| title | Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts |
| title_full | Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts |
| title_fullStr | Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts |
| title_full_unstemmed | Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts |
| title_short | Enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small-diameter artificial vascular grafts |
| title_sort | enhanced hemocompatibility and rapid magnetic anastomosis of electrospun small diameter artificial vascular grafts |
| topic | coaxial electrospinning magnetic anastomosis small-diameter vascular grafts hemocompatibility sutureless |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1331078/full |
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