Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids
We investigated the effect of 21 flavonoids in a three-dimensional in vitro system for their ability to inhibit gap formation by MCF-7 breast cancer spheroids in monolayers of lymphendothelial cells. Different representatives of the classes of flavones, flavonols, and flavanones were tested in the c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/87/3/23 |
| _version_ | 1798025375702319104 |
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| author | Julia Eichsteininger Kerstin Kirisits Claudia Smöch Christa Stadlbauer Chi Huu Nguyen Walter Jäger Ali Özmen Gerhard Ecker Georg Krupitza Liselotte Krenn |
| author_facet | Julia Eichsteininger Kerstin Kirisits Claudia Smöch Christa Stadlbauer Chi Huu Nguyen Walter Jäger Ali Özmen Gerhard Ecker Georg Krupitza Liselotte Krenn |
| author_sort | Julia Eichsteininger |
| collection | DOAJ |
| description | We investigated the effect of 21 flavonoids in a three-dimensional in vitro system for their ability to inhibit gap formation by MCF-7 breast cancer spheroids in monolayers of lymphendothelial cells. Different representatives of the classes of flavones, flavonols, and flavanones were tested in the circular chemorepellent-induced defects (CCID)-assay. Bay11-7082, a known inhibitor of CCID formation served as the positive control. This study provides the first comparison of the potential of flavonoids to suppress features influencing the intravasation of MCF-7 breast cancer cells aggregates through the lymph endothelial barrier. The most significant effects were seen after incubation with the flavones luteolin, chrysin, and apigenin. Additional hydroxylation or methoxylation in positions 6 or 8, as expected, resulted in decreased activity. The tested flavanones remained without or low efficacy. |
| first_indexed | 2024-04-11T18:17:48Z |
| format | Article |
| id | doaj.art-9a52568d42d440f4882d85e1b7f9bca9 |
| institution | Directory Open Access Journal |
| issn | 2218-0532 |
| language | English |
| last_indexed | 2024-04-11T18:17:48Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Scientia Pharmaceutica |
| spelling | doaj.art-9a52568d42d440f4882d85e1b7f9bca92022-12-22T04:09:50ZengMDPI AGScientia Pharmaceutica2218-05322019-09-018732310.3390/scipharm87030023scipharm87030023Structural Insight into the In Vitro Anti-Intravasative Properties of FlavonoidsJulia Eichsteininger0Kerstin Kirisits1Claudia Smöch2Christa Stadlbauer3Chi Huu Nguyen4Walter Jäger5Ali Özmen6Gerhard Ecker7Georg Krupitza8Liselotte Krenn9Department of Pathology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Pathology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Pathology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Pathology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Pathology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Clinical Pharmacy and Diagnostics, Faculty of Life Sciences, University of Vienna, 1090 Vienna, AustriaDepartment of Biology, Faculty of Science and Art, Adnan Menderes University, 90010 Aydin, TurkeyPharmacoinformatics Research Group, Department of Pharmaceutical Chemistry, Faculty of Life Sciences, University of Vienna, 1090 Vienna, AustriaDepartment of Pathology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Pharmacognosy, Faculty of Life Sciences, University of Vienna, 1090 Vienna, AustriaWe investigated the effect of 21 flavonoids in a three-dimensional in vitro system for their ability to inhibit gap formation by MCF-7 breast cancer spheroids in monolayers of lymphendothelial cells. Different representatives of the classes of flavones, flavonols, and flavanones were tested in the circular chemorepellent-induced defects (CCID)-assay. Bay11-7082, a known inhibitor of CCID formation served as the positive control. This study provides the first comparison of the potential of flavonoids to suppress features influencing the intravasation of MCF-7 breast cancer cells aggregates through the lymph endothelial barrier. The most significant effects were seen after incubation with the flavones luteolin, chrysin, and apigenin. Additional hydroxylation or methoxylation in positions 6 or 8, as expected, resulted in decreased activity. The tested flavanones remained without or low efficacy.https://www.mdpi.com/2218-0532/87/3/23CCID assayinhibition of gap formationflavonoidsanti-invasivebreast cancerstructure-activity relationship |
| spellingShingle | Julia Eichsteininger Kerstin Kirisits Claudia Smöch Christa Stadlbauer Chi Huu Nguyen Walter Jäger Ali Özmen Gerhard Ecker Georg Krupitza Liselotte Krenn Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids Scientia Pharmaceutica CCID assay inhibition of gap formation flavonoids anti-invasive breast cancer structure-activity relationship |
| title | Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids |
| title_full | Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids |
| title_fullStr | Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids |
| title_full_unstemmed | Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids |
| title_short | Structural Insight into the In Vitro Anti-Intravasative Properties of Flavonoids |
| title_sort | structural insight into the in vitro anti intravasative properties of flavonoids |
| topic | CCID assay inhibition of gap formation flavonoids anti-invasive breast cancer structure-activity relationship |
| url | https://www.mdpi.com/2218-0532/87/3/23 |
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