A spectroscopic study of interaction of cationic dyes with heparin
The interaction of two cationic dyes namely, acridine orange and pinacyanol chloride with an anionic polyelectrolyte, heparin, has been investigated by spectrophotometric method.The polymer induced metachromasy in the dyes resulting in the shift of the absorption maxima of the dyes towards shorter w...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Mato Grosso do Sul
2009-12-01
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| Series: | Orbital: The Electronic Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.orbital.ufms.br/index.php/dqi/article/view/68/28 |
| _version_ | 1819238909228351488 |
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| author | B. Vishalakshi R. Nandini |
| author_facet | B. Vishalakshi R. Nandini |
| author_sort | B. Vishalakshi |
| collection | DOAJ |
| description | The interaction of two cationic dyes namely, acridine orange and pinacyanol chloride with an anionic polyelectrolyte, heparin, has been investigated by spectrophotometric method.The polymer induced metachromasy in the dyes resulting in the shift of the absorption maxima of the dyes towards shorter wavelengths. The stability of the complexes formed between acridine orange and heparin was found to be lesser than that formed between pinacyanol chloride and heparin. This fact was further confirmed by reversal studies using alcohols, urea and surfactants. The interaction of acridine orange with heparin has also been investigated fluorimetrically.The interaction parameters revealed that binding between acridine orange and heparin arises due to electrostatic interaction while that between pinacyanol chloride and heparin is found to involve both electrostatic and hydrophobic forces. The effect of the structure of the dye in inducing metachromasy has also been discussed. |
| first_indexed | 2024-12-23T13:43:43Z |
| format | Article |
| id | doaj.art-9a563e563c3f469ab4cfc09de4dc03cb |
| institution | Directory Open Access Journal |
| issn | 1984-6428 |
| language | English |
| last_indexed | 2024-12-23T13:43:43Z |
| publishDate | 2009-12-01 |
| publisher | Universidade Federal de Mato Grosso do Sul |
| record_format | Article |
| series | Orbital: The Electronic Journal of Chemistry |
| spelling | doaj.art-9a563e563c3f469ab4cfc09de4dc03cb2022-12-21T17:44:47ZengUniversidade Federal de Mato Grosso do SulOrbital: The Electronic Journal of Chemistry1984-64282009-12-0114255272A spectroscopic study of interaction of cationic dyes with heparinB. VishalakshiR. NandiniThe interaction of two cationic dyes namely, acridine orange and pinacyanol chloride with an anionic polyelectrolyte, heparin, has been investigated by spectrophotometric method.The polymer induced metachromasy in the dyes resulting in the shift of the absorption maxima of the dyes towards shorter wavelengths. The stability of the complexes formed between acridine orange and heparin was found to be lesser than that formed between pinacyanol chloride and heparin. This fact was further confirmed by reversal studies using alcohols, urea and surfactants. The interaction of acridine orange with heparin has also been investigated fluorimetrically.The interaction parameters revealed that binding between acridine orange and heparin arises due to electrostatic interaction while that between pinacyanol chloride and heparin is found to involve both electrostatic and hydrophobic forces. The effect of the structure of the dye in inducing metachromasy has also been discussed.http://www.orbital.ufms.br/index.php/dqi/article/view/68/28cationic dyesmetachromatic complexheparinfluorescence quenchingstructureaggregation |
| spellingShingle | B. Vishalakshi R. Nandini A spectroscopic study of interaction of cationic dyes with heparin Orbital: The Electronic Journal of Chemistry cationic dyes metachromatic complex heparin fluorescence quenching structure aggregation |
| title | A spectroscopic study of interaction of cationic dyes with heparin |
| title_full | A spectroscopic study of interaction of cationic dyes with heparin |
| title_fullStr | A spectroscopic study of interaction of cationic dyes with heparin |
| title_full_unstemmed | A spectroscopic study of interaction of cationic dyes with heparin |
| title_short | A spectroscopic study of interaction of cationic dyes with heparin |
| title_sort | spectroscopic study of interaction of cationic dyes with heparin |
| topic | cationic dyes metachromatic complex heparin fluorescence quenching structure aggregation |
| url | http://www.orbital.ufms.br/index.php/dqi/article/view/68/28 |
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