Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection
Disruption of the perineuronal matrix has been reported in human immunodeficiency virus (HIV) encephalitis. To better understand the extent of matrix disruption during lentiviral encephalitis, we characterized the extracellular matrix (ECM) damage in brains of 12 macaques infected with simian immuno...
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| Format: | Article |
| Language: | English |
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Elsevier
2004-08-01
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| Series: | Neurobiology of Disease |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996104001044 |
| _version_ | 1818417445658951680 |
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| author | Rafael Medina-Flores Guoji Wang Stephanie J Bissel Michael Murphey-Corb Clayton A Wiley |
| author_facet | Rafael Medina-Flores Guoji Wang Stephanie J Bissel Michael Murphey-Corb Clayton A Wiley |
| author_sort | Rafael Medina-Flores |
| collection | DOAJ |
| description | Disruption of the perineuronal matrix has been reported in human immunodeficiency virus (HIV) encephalitis. To better understand the extent of matrix disruption during lentiviral encephalitis, we characterized the extracellular matrix (ECM) damage in brains of 12 macaques infected with simian immunodeficiency virus (SIV). Matrix integrity was assessed by Wisteria floribunda lectin histochemistry. Confocal microscopy was used to quantify matrix loss, macrophage infiltration, and synaptic damage. Disruption of brain ECM was present shortly after retroviral infection, preceding parenchymal macrophage infiltration. In agreement with previous observations, reduced staining of presynaptic and postsynaptic proteins in SIV encephalitis occurred concurrently with matrix abnormalities. Lentiviral infection induced microglial and macrophage expression of two ḏisintegrins and m̱etalloproteinases with ṯhrombos̱pondin motifs (ADAMTS-1 and ADAMTS-4), with high substrate specificity for matrix proteoglycans. Matrix damage is pervasive during SIV neuroinfection, which suggests interventions to conserve brain matrix proteoglycans might avert or delay retroviral-induced neurodegeneration. |
| first_indexed | 2024-12-14T12:06:54Z |
| format | Article |
| id | doaj.art-9a5683efa9654a13a9e24251ad32313d |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-14T12:06:54Z |
| publishDate | 2004-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-9a5683efa9654a13a9e24251ad32313d2022-12-21T23:01:50ZengElsevierNeurobiology of Disease1095-953X2004-08-01163604616Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfectionRafael Medina-Flores0Guoji Wang1Stephanie J Bissel2Michael Murphey-Corb3Clayton A Wiley4Division of Neuropathology, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA 15213, USADivision of Neuropathology, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA 15213, USADivision of Neuropathology, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA 15213, USADivision of Neuropathology, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA 15213, USADivision of Neuropathology, Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA 15213, USA; Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA 15213, USA; Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA 15213, USADisruption of the perineuronal matrix has been reported in human immunodeficiency virus (HIV) encephalitis. To better understand the extent of matrix disruption during lentiviral encephalitis, we characterized the extracellular matrix (ECM) damage in brains of 12 macaques infected with simian immunodeficiency virus (SIV). Matrix integrity was assessed by Wisteria floribunda lectin histochemistry. Confocal microscopy was used to quantify matrix loss, macrophage infiltration, and synaptic damage. Disruption of brain ECM was present shortly after retroviral infection, preceding parenchymal macrophage infiltration. In agreement with previous observations, reduced staining of presynaptic and postsynaptic proteins in SIV encephalitis occurred concurrently with matrix abnormalities. Lentiviral infection induced microglial and macrophage expression of two ḏisintegrins and m̱etalloproteinases with ṯhrombos̱pondin motifs (ADAMTS-1 and ADAMTS-4), with high substrate specificity for matrix proteoglycans. Matrix damage is pervasive during SIV neuroinfection, which suggests interventions to conserve brain matrix proteoglycans might avert or delay retroviral-induced neurodegeneration.http://www.sciencedirect.com/science/article/pii/S0969996104001044SIVHIV-1AIDSEncephalitisExtracellular matrixProteoglycans |
| spellingShingle | Rafael Medina-Flores Guoji Wang Stephanie J Bissel Michael Murphey-Corb Clayton A Wiley Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection Neurobiology of Disease SIV HIV-1 AIDS Encephalitis Extracellular matrix Proteoglycans |
| title | Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection |
| title_full | Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection |
| title_fullStr | Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection |
| title_full_unstemmed | Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection |
| title_short | Destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection |
| title_sort | destruction of extracellular matrix proteoglycans is pervasive in simian retroviral neuroinfection |
| topic | SIV HIV-1 AIDS Encephalitis Extracellular matrix Proteoglycans |
| url | http://www.sciencedirect.com/science/article/pii/S0969996104001044 |
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