In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine
Background: With the implementation of minimally invasive selective caries removal protocols to treat cavitated, deep carious dentine lesions, there is a need to investigate specific biochemical moiety distributions to help characterise and distinguish between infected (contaminated) and affected (d...
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MDPI AG
2022-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/3/1088 |
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| author | Mohammed Alturki Ulrica Almhöjd Garrit Koller Fiona Warburton Avijit Banerjee |
| author_facet | Mohammed Alturki Ulrica Almhöjd Garrit Koller Fiona Warburton Avijit Banerjee |
| author_sort | Mohammed Alturki |
| collection | DOAJ |
| description | Background: With the implementation of minimally invasive selective caries removal protocols to treat cavitated, deep carious dentine lesions, there is a need to investigate specific biochemical moiety distributions to help characterise and distinguish between infected (contaminated) and affected (demineralised) zones within the dentine lesion. The present in vitro investigation aimed to compare the distribution of ester functional groups (1740 cm<sup>−1</sup>) within carious dentine tissue (infected and affected dentine). The null hypothesis stipulated that there are no differences in ester function intensity/distribution within carious dentine lesions. Materials and Methods: From a total of five extracted human molar teeth with carious dentine lesions, 246 points from 10 sections of carious dentine were examined using high-resolution Raman spectroscopy and characterised into infected, affected and sound dentine. The peak intensity of the characteristic vibration mode of the ester function was calculated from sample scans. Results: Analyses indicated a statistically significant difference in the spectroscopic vibration bands of esters between the infected and affected dentine zones. Conclusion: The ester functional group is higher in intensity in the caries-infected dentine zone compared to the affected tissue. This finding could be used to develop an objective indicator for the selective operative management of carious dentine. |
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| format | Article |
| id | doaj.art-9a56a4369b9a47698b2db936813f2ecf |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T00:16:07Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-9a56a4369b9a47698b2db936813f2ecf2023-11-23T15:51:39ZengMDPI AGApplied Sciences2076-34172022-01-01123108810.3390/app12031088In Vitro Analysis of Organic Ester Functional Groups in Carious DentineMohammed Alturki0Ulrica Almhöjd1Garrit Koller2Fiona Warburton3Avijit Banerjee4Centre of Oral Clinical Translational Sciences, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKCentre of Oral Clinical Translational Sciences, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKCentre for Host Microbiome Interactions, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKFaculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKCentre of Oral Clinical Translational Sciences, Faculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKBackground: With the implementation of minimally invasive selective caries removal protocols to treat cavitated, deep carious dentine lesions, there is a need to investigate specific biochemical moiety distributions to help characterise and distinguish between infected (contaminated) and affected (demineralised) zones within the dentine lesion. The present in vitro investigation aimed to compare the distribution of ester functional groups (1740 cm<sup>−1</sup>) within carious dentine tissue (infected and affected dentine). The null hypothesis stipulated that there are no differences in ester function intensity/distribution within carious dentine lesions. Materials and Methods: From a total of five extracted human molar teeth with carious dentine lesions, 246 points from 10 sections of carious dentine were examined using high-resolution Raman spectroscopy and characterised into infected, affected and sound dentine. The peak intensity of the characteristic vibration mode of the ester function was calculated from sample scans. Results: Analyses indicated a statistically significant difference in the spectroscopic vibration bands of esters between the infected and affected dentine zones. Conclusion: The ester functional group is higher in intensity in the caries-infected dentine zone compared to the affected tissue. This finding could be used to develop an objective indicator for the selective operative management of carious dentine.https://www.mdpi.com/2076-3417/12/3/1088caries-infectedcaries-affecteddentineRaman spectroscopylucifer yellowamide I |
| spellingShingle | Mohammed Alturki Ulrica Almhöjd Garrit Koller Fiona Warburton Avijit Banerjee In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine Applied Sciences caries-infected caries-affected dentine Raman spectroscopy lucifer yellow amide I |
| title | In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine |
| title_full | In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine |
| title_fullStr | In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine |
| title_full_unstemmed | In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine |
| title_short | In Vitro Analysis of Organic Ester Functional Groups in Carious Dentine |
| title_sort | in vitro analysis of organic ester functional groups in carious dentine |
| topic | caries-infected caries-affected dentine Raman spectroscopy lucifer yellow amide I |
| url | https://www.mdpi.com/2076-3417/12/3/1088 |
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