Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology.
Many organs, including salivary glands, lung, and kidney, are formed by epithelial branching during embryonic development. Branching morphogenesis occurs via either local outgrowths or the formation of clefts that subdivide epithelia into buds. This process is promoted by various factors, but the me...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4398443?pdf=render |
| _version_ | 1811270623792463872 |
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| author | Aya Obana-Koshino Hitomi Ono Jiro Miura Manabu Sakai Hitoshi Uchida Wataru Nakamura Kanji Nohara Yusuke Maruyama Atsuhiko Hattori Takayoshi Sakai |
| author_facet | Aya Obana-Koshino Hitomi Ono Jiro Miura Manabu Sakai Hitoshi Uchida Wataru Nakamura Kanji Nohara Yusuke Maruyama Atsuhiko Hattori Takayoshi Sakai |
| author_sort | Aya Obana-Koshino |
| collection | DOAJ |
| description | Many organs, including salivary glands, lung, and kidney, are formed by epithelial branching during embryonic development. Branching morphogenesis occurs via either local outgrowths or the formation of clefts that subdivide epithelia into buds. This process is promoted by various factors, but the mechanism of branching morphogenesis is not fully understood. Here we have defined melatonin as a potential negative regulator or "brake" of branching morphogenesis, shown that the levels of it and its receptors decline when branching morphogenesis begins, and identified the process that it regulates. Melatonin has various physiological functions, including circadian rhythm regulation, free-radical scavenging, and gonadal development. Furthermore, melatonin is present in saliva and may have an important physiological role in the oral cavity. In this study, we found that the melatonin receptor is highly expressed on the acinar epithelium of the embryonic submandibular gland. We also found that exogenous melatonin reduces salivary gland size and inhibits branching morphogenesis. We suggest that this inhibition does not depend on changes in either proliferation or apoptosis, but rather relates to changes in epithelial cell adhesion and morphology. In summary, we have demonstrated a novel function of melatonin in organ formation during embryonic development. |
| first_indexed | 2024-04-12T22:05:33Z |
| format | Article |
| id | doaj.art-9a57d637e08a4a9790893934ec02870b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T22:05:33Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9a57d637e08a4a9790893934ec02870b2022-12-22T03:14:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e011996010.1371/journal.pone.0119960Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology.Aya Obana-KoshinoHitomi OnoJiro MiuraManabu SakaiHitoshi UchidaWataru NakamuraKanji NoharaYusuke MaruyamaAtsuhiko HattoriTakayoshi SakaiMany organs, including salivary glands, lung, and kidney, are formed by epithelial branching during embryonic development. Branching morphogenesis occurs via either local outgrowths or the formation of clefts that subdivide epithelia into buds. This process is promoted by various factors, but the mechanism of branching morphogenesis is not fully understood. Here we have defined melatonin as a potential negative regulator or "brake" of branching morphogenesis, shown that the levels of it and its receptors decline when branching morphogenesis begins, and identified the process that it regulates. Melatonin has various physiological functions, including circadian rhythm regulation, free-radical scavenging, and gonadal development. Furthermore, melatonin is present in saliva and may have an important physiological role in the oral cavity. In this study, we found that the melatonin receptor is highly expressed on the acinar epithelium of the embryonic submandibular gland. We also found that exogenous melatonin reduces salivary gland size and inhibits branching morphogenesis. We suggest that this inhibition does not depend on changes in either proliferation or apoptosis, but rather relates to changes in epithelial cell adhesion and morphology. In summary, we have demonstrated a novel function of melatonin in organ formation during embryonic development.http://europepmc.org/articles/PMC4398443?pdf=render |
| spellingShingle | Aya Obana-Koshino Hitomi Ono Jiro Miura Manabu Sakai Hitoshi Uchida Wataru Nakamura Kanji Nohara Yusuke Maruyama Atsuhiko Hattori Takayoshi Sakai Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology. PLoS ONE |
| title | Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology. |
| title_full | Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology. |
| title_fullStr | Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology. |
| title_full_unstemmed | Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology. |
| title_short | Melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology. |
| title_sort | melatonin inhibits embryonic salivary gland branching morphogenesis by regulating both epithelial cell adhesion and morphology |
| url | http://europepmc.org/articles/PMC4398443?pdf=render |
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