Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip
Summary: This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722004075 |
| _version_ | 1817976643900145664 |
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| author | Kazumi Koba Kyoko Yarimizu So Fujiyoshi Kyoichi Oshiro Yoshikazu Wakizaka Masayo Takano Fumito Maruyama |
| author_facet | Kazumi Koba Kyoko Yarimizu So Fujiyoshi Kyoichi Oshiro Yoshikazu Wakizaka Masayo Takano Fumito Maruyama |
| author_sort | Kazumi Koba |
| collection | DOAJ |
| description | Summary: This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chip installation, sample and buffer filling, flow rate and cell concentration adjustments, and contamination sources increases the efficiency of target viable cell collection. Such instructions, although trivial, are critical for achieving cell separation.For complete details on the use and execution of this protocol, please refer to Oshiro et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T22:06:29Z |
| format | Article |
| id | doaj.art-9a5a6eec509e40fe8a4e7200f2620442 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T22:06:29Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-9a5a6eec509e40fe8a4e7200f26204422022-12-22T02:27:56ZengElsevierSTAR Protocols2666-16672022-09-0133101527Protocol of living cell separation using the microfluidic dielectrophoresis integrated chipKazumi Koba0Kyoko Yarimizu1So Fujiyoshi2Kyoichi Oshiro3Yoshikazu Wakizaka4Masayo Takano5Fumito Maruyama6Microbial Genomics and Ecology, The IDEC Institute, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, JapanMicrobial Genomics and Ecology, The IDEC Institute, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, JapanMicrobial Genomics and Ecology, The IDEC Institute, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, JapanAFI Corporation, Medical Innovation Center Bldg. 2nd Floor of Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto City 606-8507, JapanAFI Corporation, Medical Innovation Center Bldg. 2nd Floor of Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto City 606-8507, JapanAFI Corporation, Medical Innovation Center Bldg. 2nd Floor of Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto City 606-8507, JapanMicrobial Genomics and Ecology, The IDEC Institute, Hiroshima University, 1-3-2 Kagamiyama, Higashi-Hiroshima City, Hiroshima 739-8511, Japan; Corresponding authorSummary: This protocol demonstrates the separation of living cells with the microfluidic dielectrophoresis chip, using the Jurkat cell as a model. The successful living cell separation lies in familiarity with the detailed tips, which are aided by this stepwise protocol. The knowledge of correct chip installation, sample and buffer filling, flow rate and cell concentration adjustments, and contamination sources increases the efficiency of target viable cell collection. Such instructions, although trivial, are critical for achieving cell separation.For complete details on the use and execution of this protocol, please refer to Oshiro et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722004075Cell BiologyCell isolationCell separation/fractionationBiotechnology and bioengineering |
| spellingShingle | Kazumi Koba Kyoko Yarimizu So Fujiyoshi Kyoichi Oshiro Yoshikazu Wakizaka Masayo Takano Fumito Maruyama Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip STAR Protocols Cell Biology Cell isolation Cell separation/fractionation Biotechnology and bioengineering |
| title | Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip |
| title_full | Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip |
| title_fullStr | Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip |
| title_full_unstemmed | Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip |
| title_short | Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip |
| title_sort | protocol of living cell separation using the microfluidic dielectrophoresis integrated chip |
| topic | Cell Biology Cell isolation Cell separation/fractionation Biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722004075 |
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