Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano
<p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US"><span style="fon...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2011-08-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22940 |
| _version_ | 1817992666419298304 |
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| author | Esther Casablanca Alarcón Neida Ríos Manríquez Enrique Terrazas Siles María Teresa Álvarez Aliaga |
| author_facet | Esther Casablanca Alarcón Neida Ríos Manríquez Enrique Terrazas Siles María Teresa Álvarez Aliaga |
| author_sort | Esther Casablanca Alarcón |
| collection | DOAJ |
| description | <p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman;"><span style="font-size: small;">β-glucoside production by thermophilic bacteria indigenous the bolivian altiplano culture</span></span></span></strong><span style="font-family: Times New Roman;"><span style="font-size: small;"><span lang="ES"> </span></span></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES"><span style="font-family: Times New Roman;"><span style="font-size: small;">Resumen </span></span></span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES"></span></strong></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt; tab-stops: 9.0pt 18.0pt;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">Las β-glucosidasas son enzimas que</span><span lang="ES"> poseen actividad hidrolítica y transferasa o transglucosidasa. Tienen diversas aplicaciones; en </span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">la biosíntesis de oligosacáridos, producción de etanol utilizando residuos agrícolas y en la industria de vinos. La aplicación industrial, sin embargo, requiere estabilidad a temperaturas elevadas, por lo que los microorganismos termófilos tienen gran interés. El propósito de esta investigación es el de optimizar el medio de cultivo anaerobio de bacterias termófilas, para aumentar la producción de β-glucosidasas. Esta enzima es producida por tres aislados bacterianos: FT3, 2B y P5 los cuales fueron aislados de la región andina de Bolivia. El aislado bacteriano FT3 mostró una actividad β-glucosidasa de 0,35</span> <span lang="ES">[UI/mL]</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">. Se tomaron como variables dentro de la optimización del medio de cultivo las fuentes de nitrógeno y de carbono, y el pH. Así también se probaron dos sistemas de cultivo: células libres y encapsuladas. Empleando extracto de levadura como fuente de nitrógeno se obtuvo una actividad de 0,52 [UI/mL]. En la optimización del pH del medio de cultivo se obtuvo una actividad de 0,81 </span><span lang="ES">[UI/mL] a pH 5.</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;"> Como fuente de carbono se eligieron los hidrolizados de paja de trigo y paja de quinoa llegándose a obtener actividades de 1,27 y 1,34 </span><span lang="ES">[UI/mL] respectivamente. Se estableció que la localización celular de la enzima </span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">β-glucosidasa es extracelular y presenta estabilidad hasta una temperatura de 80 </span></span><span style="font-family: ">°</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;"><span style="font-family: Times New Roman;">C y un pH de 7.</span></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><em style="mso-bidi-font-style: normal;"><span lang="ES"></span></em></strong></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES">Palabras clave: </span></strong><span lang="ES">optimización</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">,</span> <span lang="ES">células inmobilizadas, bacterias extremófilas. </span></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span lang="ES"><span style="font-family: Times New Roman; font-size: small;"> </span></span><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong><span style="mso-ansi-language: EN-US;" lang="EN-US">Abstract</span></strong></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><strong><span style="mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman; font-size: small;"> </span></span></strong><span style="font-family: Times New Roman;"><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">The </span></span><span class="longtext1"><span style="background: white; font-size: 10pt;" lang="ES">β</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">-glucosidases possess hydrolytic and transferase activity or transglucosidase. They have various applications; such as biosynthesis of oligosaccharides, production of ethanol using agricultural residues and wine industry</span></span><span style="color: black; mso-ansi-language: EN-GB;" lang="EN-GB"><span style="font-size: small;">. However for industrial application, stability to high temperatures is needed. Therefore a great interesting in the thermophile microorganism study exist.</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US"> The purpose of this research is to optimize the culture medium of thermophilic anaerobic bacteria to increase the production of </span></span><span class="longtext1"><span style="background: white; font-size: 10pt;" lang="ES">β</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">-glucosidase. This enzyme is produced by three isolate bacterial FT3, 2B and P5 which were isolated from the Andean region of Bolivia. </span></span><span class="longtext1"><span style="font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">FT3 isolate showed </span></span><span class="longtext1"><span style="font-size: 10pt;" lang="ES">β</span></span><span class="longtext1"><span style="font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">-glucosidase activity of 0.35 [IU/mL]. In regards to the optimization<span style="background: white;"> of culture medium variables such as nitrogen source, carbon source and pH were taken into account and also the combination with free and encapsulated bacterial cells. Yeast extract was the selected source of nitrogen obtaining an activity of 0.52 [IU/ mL]. </span></span></span><span style="mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US"><span style="font-size: small;">The optimal pH was 5 obtaining an activity of 0.81 [IU/mL].</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US"> The selected carbon source was the hydrolyzed wheat straw and quinoa straw </span></span></span><span style="mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US"><span style="font-size: small;"><span style="font-family: Times New Roman;">obtaining activities of 1.27 and 1.34 [IU/mL], respectively. The cellular localization of β-glucosidase enzyme is extracellular and provides stability to temperature of </span><span style="font-family: Times New Roman;">80 </span><span style="font-family: ">°</span><span style="font-family: Times New Roman;">C</span><span style="font-family: Times New Roman;"> and stability at pH 7.</span></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="mso-ansi-language: EN-GB; mso-bidi-font-weight: bold;" lang="EN-GB"></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><span style="font-family: Times New Roman;"><span style="font-size: small;"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US">Key words</span></strong><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US">: </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">Optimization,</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-US"> </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">immobilized</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-US"> </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">cells,</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-US"> </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">extremophile</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-GB"> bacteria.</span></span></span></p> |
| first_indexed | 2024-04-14T01:29:12Z |
| format | Article |
| id | doaj.art-9a5b093329f04808a9e4cff6d3bfec0c |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-04-14T01:29:12Z |
| publishDate | 2011-08-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-9a5b093329f04808a9e4cff6d3bfec0c2022-12-22T02:20:16ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582011-08-01131667220474Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano bolivianoEsther Casablanca Alarcón0Neida Ríos Manríquez1Enrique Terrazas Siles2María Teresa Álvarez Aliaga3InvestigadorInvestigadorInvestigadorInvestigador<p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman;"><span style="font-size: small;">β-glucoside production by thermophilic bacteria indigenous the bolivian altiplano culture</span></span></span></strong><span style="font-family: Times New Roman;"><span style="font-size: small;"><span lang="ES"> </span></span></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES"><span style="font-family: Times New Roman;"><span style="font-size: small;">Resumen </span></span></span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES"></span></strong></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt; tab-stops: 9.0pt 18.0pt;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">Las β-glucosidasas son enzimas que</span><span lang="ES"> poseen actividad hidrolítica y transferasa o transglucosidasa. Tienen diversas aplicaciones; en </span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">la biosíntesis de oligosacáridos, producción de etanol utilizando residuos agrícolas y en la industria de vinos. La aplicación industrial, sin embargo, requiere estabilidad a temperaturas elevadas, por lo que los microorganismos termófilos tienen gran interés. El propósito de esta investigación es el de optimizar el medio de cultivo anaerobio de bacterias termófilas, para aumentar la producción de β-glucosidasas. Esta enzima es producida por tres aislados bacterianos: FT3, 2B y P5 los cuales fueron aislados de la región andina de Bolivia. El aislado bacteriano FT3 mostró una actividad β-glucosidasa de 0,35</span> <span lang="ES">[UI/mL]</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">. Se tomaron como variables dentro de la optimización del medio de cultivo las fuentes de nitrógeno y de carbono, y el pH. Así también se probaron dos sistemas de cultivo: células libres y encapsuladas. Empleando extracto de levadura como fuente de nitrógeno se obtuvo una actividad de 0,52 [UI/mL]. En la optimización del pH del medio de cultivo se obtuvo una actividad de 0,81 </span><span lang="ES">[UI/mL] a pH 5.</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;"> Como fuente de carbono se eligieron los hidrolizados de paja de trigo y paja de quinoa llegándose a obtener actividades de 1,27 y 1,34 </span><span lang="ES">[UI/mL] respectivamente. Se estableció que la localización celular de la enzima </span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">β-glucosidasa es extracelular y presenta estabilidad hasta una temperatura de 80 </span></span><span style="font-family: ">°</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;"><span style="font-family: Times New Roman;">C y un pH de 7.</span></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><em style="mso-bidi-font-style: normal;"><span lang="ES"></span></em></strong></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong style="mso-bidi-font-weight: normal;"><span lang="ES">Palabras clave: </span></strong><span lang="ES">optimización</span><span style="mso-ansi-language: ES-CO; mso-bidi-font-weight: bold;">,</span> <span lang="ES">células inmobilizadas, bacterias extremófilas. </span></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span lang="ES"><span style="font-family: Times New Roman; font-size: small;"> </span></span><span style="font-size: small;"><span style="font-family: Times New Roman;"><strong><span style="mso-ansi-language: EN-US;" lang="EN-US">Abstract</span></strong></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><strong><span style="mso-ansi-language: EN-US;" lang="EN-US"><span style="font-family: Times New Roman; font-size: small;"> </span></span></strong><span style="font-family: Times New Roman;"><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">The </span></span><span class="longtext1"><span style="background: white; font-size: 10pt;" lang="ES">β</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">-glucosidases possess hydrolytic and transferase activity or transglucosidase. They have various applications; such as biosynthesis of oligosaccharides, production of ethanol using agricultural residues and wine industry</span></span><span style="color: black; mso-ansi-language: EN-GB;" lang="EN-GB"><span style="font-size: small;">. However for industrial application, stability to high temperatures is needed. Therefore a great interesting in the thermophile microorganism study exist.</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US"> The purpose of this research is to optimize the culture medium of thermophilic anaerobic bacteria to increase the production of </span></span><span class="longtext1"><span style="background: white; font-size: 10pt;" lang="ES">β</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">-glucosidase. This enzyme is produced by three isolate bacterial FT3, 2B and P5 which were isolated from the Andean region of Bolivia. </span></span><span class="longtext1"><span style="font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">FT3 isolate showed </span></span><span class="longtext1"><span style="font-size: 10pt;" lang="ES">β</span></span><span class="longtext1"><span style="font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US">-glucosidase activity of 0.35 [IU/mL]. In regards to the optimization<span style="background: white;"> of culture medium variables such as nitrogen source, carbon source and pH were taken into account and also the combination with free and encapsulated bacterial cells. Yeast extract was the selected source of nitrogen obtaining an activity of 0.52 [IU/ mL]. </span></span></span><span style="mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US"><span style="font-size: small;">The optimal pH was 5 obtaining an activity of 0.81 [IU/mL].</span></span><span class="longtext1"><span style="background: white; font-size: 10pt; mso-ansi-language: EN-US;" lang="EN-US"> The selected carbon source was the hydrolyzed wheat straw and quinoa straw </span></span></span><span style="mso-ansi-language: EN-US; mso-bidi-font-weight: bold;" lang="EN-US"><span style="font-size: small;"><span style="font-family: Times New Roman;">obtaining activities of 1.27 and 1.34 [IU/mL], respectively. The cellular localization of β-glucosidase enzyme is extracellular and provides stability to temperature of </span><span style="font-family: Times New Roman;">80 </span><span style="font-family: ">°</span><span style="font-family: Times New Roman;">C</span><span style="font-family: Times New Roman;"> and stability at pH 7.</span></span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="mso-ansi-language: EN-GB; mso-bidi-font-weight: bold;" lang="EN-GB"></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><span style="font-family: Times New Roman;"><span style="font-size: small;"><strong style="mso-bidi-font-weight: normal;"><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US">Key words</span></strong><span style="color: black; mso-ansi-language: EN-US;" lang="EN-US">: </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">Optimization,</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-US"> </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">immobilized</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-US"> </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">cells,</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-US"> </span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-US;" lang="EN-US">extremophile</span></span><span class="shorttext1"><span style="color: black; font-size: 13pt; mso-ansi-language: EN-GB;" lang="EN-GB"> bacteria.</span></span></span></p>http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22940optimizacióncélulas inmobilizadasbacterias extremófilasOptimizationimmobilized cellsextremophile bacteria |
| spellingShingle | Esther Casablanca Alarcón Neida Ríos Manríquez Enrique Terrazas Siles María Teresa Álvarez Aliaga Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano Revista Colombiana de Biotecnología optimización células inmobilizadas bacterias extremófilas Optimization immobilized cells extremophile bacteria |
| title | Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano |
| title_full | Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano |
| title_fullStr | Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano |
| title_full_unstemmed | Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano |
| title_short | Producción de β-glucosidasas por cultivos de bacterias termófilas indígenas del altiplano boliviano |
| title_sort | produccion de β glucosidasas por cultivos de bacterias termofilas indigenas del altiplano boliviano |
| topic | optimización células inmobilizadas bacterias extremófilas Optimization immobilized cells extremophile bacteria |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22940 |
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