Proteomic analysis of fipronil-induced molecular defects in spermatozoa
Abstract The phenylpyrazole insecticide fipronil has wide-ranging applications from agriculture to public health to control undesirable organisms. However, several studies have reported the residual environmental hazards of fipronil and demonstrated its harmful effects even in mammalian reproduction...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-57876-4 |
| _version_ | 1827291528516599808 |
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| author | Jeong-Won Bae Woo-Sung Kwon |
| author_facet | Jeong-Won Bae Woo-Sung Kwon |
| author_sort | Jeong-Won Bae |
| collection | DOAJ |
| description | Abstract The phenylpyrazole insecticide fipronil has wide-ranging applications from agriculture to public health to control undesirable organisms. However, several studies have reported the residual environmental hazards of fipronil and demonstrated its harmful effects even in mammalian reproduction. Therefore, this study was conducted to demonstrate the mode of action of fipronil on mouse spermatozoa. We treated fipronil to spermatozoa and performed comprehensive function evaluations. Moreover, proteomic analyses were conducted to identify the alteration of protein expression levels in spermatozoa. Most of sperm motility and kinematic parameters and intracellular ATP levels were diminished, and the spontaneous acrosome reaction was promoted after treatment with fipronil. Proteomic analyses revealed altered expression levels of 14 proteins after treatment. These proteins have been reported to be associated with sperm-specific pathways, prominently the cytoskeleton of the sperm, “9 + 2” axoneme composition, metabolism, and fertility. Collectively, our results showed that fipronil alters sperm functional-related proteins and therefore influences male fertility. This study elucidates the possible reproductive toxic hazards associated with male infertility through aberrant suppression of sperm proteins. |
| first_indexed | 2024-04-24T12:39:36Z |
| format | Article |
| id | doaj.art-9a5d96b93e7c43a6a9f5e4c12c9bb444 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T12:39:36Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9a5d96b93e7c43a6a9f5e4c12c9bb4442024-04-07T11:19:04ZengNature PortfolioScientific Reports2045-23222024-04-0114111210.1038/s41598-024-57876-4Proteomic analysis of fipronil-induced molecular defects in spermatozoaJeong-Won Bae0Woo-Sung Kwon1Department of Animal Science and Biotechnology, Kyungpook National UniversityDepartment of Animal Science and Biotechnology, Kyungpook National UniversityAbstract The phenylpyrazole insecticide fipronil has wide-ranging applications from agriculture to public health to control undesirable organisms. However, several studies have reported the residual environmental hazards of fipronil and demonstrated its harmful effects even in mammalian reproduction. Therefore, this study was conducted to demonstrate the mode of action of fipronil on mouse spermatozoa. We treated fipronil to spermatozoa and performed comprehensive function evaluations. Moreover, proteomic analyses were conducted to identify the alteration of protein expression levels in spermatozoa. Most of sperm motility and kinematic parameters and intracellular ATP levels were diminished, and the spontaneous acrosome reaction was promoted after treatment with fipronil. Proteomic analyses revealed altered expression levels of 14 proteins after treatment. These proteins have been reported to be associated with sperm-specific pathways, prominently the cytoskeleton of the sperm, “9 + 2” axoneme composition, metabolism, and fertility. Collectively, our results showed that fipronil alters sperm functional-related proteins and therefore influences male fertility. This study elucidates the possible reproductive toxic hazards associated with male infertility through aberrant suppression of sperm proteins.https://doi.org/10.1038/s41598-024-57876-4Pesticide-affected spermatozoaDifferentially expressed proteinsMale infertility |
| spellingShingle | Jeong-Won Bae Woo-Sung Kwon Proteomic analysis of fipronil-induced molecular defects in spermatozoa Scientific Reports Pesticide-affected spermatozoa Differentially expressed proteins Male infertility |
| title | Proteomic analysis of fipronil-induced molecular defects in spermatozoa |
| title_full | Proteomic analysis of fipronil-induced molecular defects in spermatozoa |
| title_fullStr | Proteomic analysis of fipronil-induced molecular defects in spermatozoa |
| title_full_unstemmed | Proteomic analysis of fipronil-induced molecular defects in spermatozoa |
| title_short | Proteomic analysis of fipronil-induced molecular defects in spermatozoa |
| title_sort | proteomic analysis of fipronil induced molecular defects in spermatozoa |
| topic | Pesticide-affected spermatozoa Differentially expressed proteins Male infertility |
| url | https://doi.org/10.1038/s41598-024-57876-4 |
| work_keys_str_mv | AT jeongwonbae proteomicanalysisoffipronilinducedmoleculardefectsinspermatozoa AT woosungkwon proteomicanalysisoffipronilinducedmoleculardefectsinspermatozoa |