Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage
Aim: Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed in Bos indicus affecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene express...
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| Format: | Article |
| Language: | English |
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Veterinary World
2017-01-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.10/January-2017/8.pdf |
| _version_ | 1818739173651120128 |
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| author | Sharadindu Shil R. S. Joshi C. G. Joshi A. K. Patel Ravi K. Shah Namrata Patel Subhash J. Jakhesara Sumana Kundu Bhaskar Reddy P. G. Koringa D. N. Rank |
| author_facet | Sharadindu Shil R. S. Joshi C. G. Joshi A. K. Patel Ravi K. Shah Namrata Patel Subhash J. Jakhesara Sumana Kundu Bhaskar Reddy P. G. Koringa D. N. Rank |
| author_sort | Sharadindu Shil |
| collection | DOAJ |
| description | Aim: Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed in Bos indicus affecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene expression in between horn’s SCC tissue and its early passage primary culture using next generation sequencing was the aim of this study.
Materials and Methods: Whole transcriptome sequencing of horn’s SCC tissue and its early passage cells using Ion Torrent PGM were done. Comparative expression and analysis of different genes and pathways related to cancer and biological processes associated with malignancy, proliferating capacity, differentiation, apoptosis, senescence, adhesion, cohesion, migration, invasion, angiogenesis, and metabolic pathways were identified.
Results: Up-regulated genes in SCC of horn’s early passage cells were involved in transporter activity, catalytic activity, nucleic acid binding transcription factor activity, biogenesis, cellular processes, biological regulation and localization and the down-regulated genes mainly were involved in focal adhesion, extracellular matrix receptor interaction and spliceosome activity.
Conclusion: The experiment revealed similar transcriptomic nature of horn’s SCC tissue and its early passage cells. |
| first_indexed | 2024-12-18T01:20:38Z |
| format | Article |
| id | doaj.art-9a5eae7e51b141adb43d78c19b2de95d |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-18T01:20:38Z |
| publishDate | 2017-01-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-9a5eae7e51b141adb43d78c19b2de95d2022-12-21T21:25:51ZengVeterinary WorldVeterinary World0972-89882231-09162017-01-01101385510.14202/vetworld.2017.38-55Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stageSharadindu Shil0R. S. Joshi1C. G. Joshi2A. K. Patel3Ravi K. Shah4Namrata Patel5Subhash J. Jakhesara6Sumana Kundu7Bhaskar Reddy8P. G. Koringa9D. N. Rank10Veterinary Officer (WBAH & VS), West Bengal Animal Resources Development Department, Bankura - 772 152 , West Bengal, India; Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Hester Biosciences Limited, Ahmedabad, Gujarat, India.Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Veterinary Officer, MVC Sarenga, Government of West Bengal, Bankura, West Bengal, India.Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Department of Animal Biotechnology, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Department of Animal Genetics & Breeding, College of Veterinary Sciences and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India.Aim: Squamous cell carcinoma or SCC of horn in bovines (bovine horn core carcinoma) frequently observed in Bos indicus affecting almost 1% of cattle population. Freshly isolated primary epithelial cells may be closely related to the malignant epithelial cells of the tumor. Comparison of gene expression in between horn’s SCC tissue and its early passage primary culture using next generation sequencing was the aim of this study. Materials and Methods: Whole transcriptome sequencing of horn’s SCC tissue and its early passage cells using Ion Torrent PGM were done. Comparative expression and analysis of different genes and pathways related to cancer and biological processes associated with malignancy, proliferating capacity, differentiation, apoptosis, senescence, adhesion, cohesion, migration, invasion, angiogenesis, and metabolic pathways were identified. Results: Up-regulated genes in SCC of horn’s early passage cells were involved in transporter activity, catalytic activity, nucleic acid binding transcription factor activity, biogenesis, cellular processes, biological regulation and localization and the down-regulated genes mainly were involved in focal adhesion, extracellular matrix receptor interaction and spliceosome activity. Conclusion: The experiment revealed similar transcriptomic nature of horn’s SCC tissue and its early passage cells.http://www.veterinaryworld.org/Vol.10/January-2017/8.pdfcummerbundgene ontologyprimary cultureRNA-sequencingsquamous cell carcinoma of horntranscriptome profiling |
| spellingShingle | Sharadindu Shil R. S. Joshi C. G. Joshi A. K. Patel Ravi K. Shah Namrata Patel Subhash J. Jakhesara Sumana Kundu Bhaskar Reddy P. G. Koringa D. N. Rank Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage Veterinary World cummerbund gene ontology primary culture RNA-sequencing squamous cell carcinoma of horn transcriptome profiling |
| title | Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage |
| title_full | Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage |
| title_fullStr | Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage |
| title_full_unstemmed | Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage |
| title_short | Transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage |
| title_sort | transcriptomic comparison of primary bovine horn core carcinoma culture and parental tissue at early stage |
| topic | cummerbund gene ontology primary culture RNA-sequencing squamous cell carcinoma of horn transcriptome profiling |
| url | http://www.veterinaryworld.org/Vol.10/January-2017/8.pdf |
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