Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i>
Glutaredoxin (Grx) is a glutathione-dependent oxidoreductase that is an important component of the redox system in organisms. However, there is a serious lack of sequence information and functional validation related to Grx in crustaceans. In this study, a novel Grx was identified in <i>Penaeu...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1857 |
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| author | Rui Fan Yundong Li Qibin Yang Song Jiang Jianhua Huang Lishi Yang Xu Chen Falin Zhou Shigui Jiang |
| author_facet | Rui Fan Yundong Li Qibin Yang Song Jiang Jianhua Huang Lishi Yang Xu Chen Falin Zhou Shigui Jiang |
| author_sort | Rui Fan |
| collection | DOAJ |
| description | Glutaredoxin (Grx) is a glutathione-dependent oxidoreductase that is an important component of the redox system in organisms. However, there is a serious lack of sequence information and functional validation related to Grx in crustaceans. In this study, a novel Grx was identified in <i>Penaeus monodon</i> (<i>PmGrx2</i>). The full-length cDNA of <i>PmGrx2</i> is 998 bp, with an open reading frame (ORF) of 441 bp, encoding 119 amino acids. Sequence alignment showed that <i>PmGrx2</i> had the highest identity with Grx2 of <i>Penaeus vannamei</i> at 96.64% and clustered with Grx2 of other crustaceans. Quantitative real-time PCR (qRT-PCR) analysis showed that <i>PmGrx2</i> was expressed in all examined tissues, with higher expression levels in the stomach and testis. <i>PmGrx2</i> was continuously expressed during development and had the highest expression level in the zygote stage. Both ammonia-N stress and bacterial infection could differentially induce the expression of <i>PmGrx2</i> in hepatopancreas and gills. When <i>PmGrx2</i> was inhibited, the expression of antioxidant enzymes was suppressed, the degree of apoptosis increased, and the GSH content decreased with the prolongation of ammonia-N stress. Inhibition of <i>PmGrx2</i> resulted in shrimp being exposed to a greater risk of oxidative damage. In addition, an SNP locus was screened on the exons of <i>PmGrx2</i> that was significantly associated with an ammonia-N-stress-tolerance trait. This study suggests that <i>PmGrx2</i> is involved in redox regulation and plays an important role in shrimps’ resistance to marine environmental stresses. |
| first_indexed | 2024-03-09T20:49:45Z |
| format | Article |
| id | doaj.art-9a5ec90134774648a176e54d9520b2e7 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:45Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-9a5ec90134774648a176e54d9520b2e72023-11-23T22:36:51ZengMDPI AGAntioxidants2076-39212022-09-011110185710.3390/antiox11101857Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i>Rui Fan0Yundong Li1Qibin Yang2Song Jiang3Jianhua Huang4Lishi Yang5Xu Chen6Falin Zhou7Shigui Jiang8Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaKey Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaGlutaredoxin (Grx) is a glutathione-dependent oxidoreductase that is an important component of the redox system in organisms. However, there is a serious lack of sequence information and functional validation related to Grx in crustaceans. In this study, a novel Grx was identified in <i>Penaeus monodon</i> (<i>PmGrx2</i>). The full-length cDNA of <i>PmGrx2</i> is 998 bp, with an open reading frame (ORF) of 441 bp, encoding 119 amino acids. Sequence alignment showed that <i>PmGrx2</i> had the highest identity with Grx2 of <i>Penaeus vannamei</i> at 96.64% and clustered with Grx2 of other crustaceans. Quantitative real-time PCR (qRT-PCR) analysis showed that <i>PmGrx2</i> was expressed in all examined tissues, with higher expression levels in the stomach and testis. <i>PmGrx2</i> was continuously expressed during development and had the highest expression level in the zygote stage. Both ammonia-N stress and bacterial infection could differentially induce the expression of <i>PmGrx2</i> in hepatopancreas and gills. When <i>PmGrx2</i> was inhibited, the expression of antioxidant enzymes was suppressed, the degree of apoptosis increased, and the GSH content decreased with the prolongation of ammonia-N stress. Inhibition of <i>PmGrx2</i> resulted in shrimp being exposed to a greater risk of oxidative damage. In addition, an SNP locus was screened on the exons of <i>PmGrx2</i> that was significantly associated with an ammonia-N-stress-tolerance trait. This study suggests that <i>PmGrx2</i> is involved in redox regulation and plays an important role in shrimps’ resistance to marine environmental stresses.https://www.mdpi.com/2076-3921/11/10/1857glutaredoxin<i>Penaeus monodon</i>oxidative stressSNPs |
| spellingShingle | Rui Fan Yundong Li Qibin Yang Song Jiang Jianhua Huang Lishi Yang Xu Chen Falin Zhou Shigui Jiang Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> Antioxidants glutaredoxin <i>Penaeus monodon</i> oxidative stress SNPs |
| title | Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> |
| title_full | Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> |
| title_fullStr | Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> |
| title_full_unstemmed | Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> |
| title_short | Expression Analysis of a Novel Oxidoreductase Glutaredoxin 2 in Black Tiger Shrimp, <i>Penaeus monodon</i> |
| title_sort | expression analysis of a novel oxidoreductase glutaredoxin 2 in black tiger shrimp i penaeus monodon i |
| topic | glutaredoxin <i>Penaeus monodon</i> oxidative stress SNPs |
| url | https://www.mdpi.com/2076-3921/11/10/1857 |
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