Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering
Abstract We aimed to construct a biodegradable transparent scaffold for culturing corneal endothelial cells by incorporating chitosan nanoparticles (CSNPs) into chitosan/polycaprolactone (PCL) membranes. Various ratios of CSNP/PCL were prepared in the presence of constant concentration of chitosan a...
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| Format: | Article |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-86340-w |
| _version_ | 1818432337510137856 |
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| author | Tahereh Tayebi Alireza Baradaran-Rafii Abbas Hajifathali Azam Rahimpour Hakimeh Zali Alireza Shaabani Hassan Niknejad |
| author_facet | Tahereh Tayebi Alireza Baradaran-Rafii Abbas Hajifathali Azam Rahimpour Hakimeh Zali Alireza Shaabani Hassan Niknejad |
| author_sort | Tahereh Tayebi |
| collection | DOAJ |
| description | Abstract We aimed to construct a biodegradable transparent scaffold for culturing corneal endothelial cells by incorporating chitosan nanoparticles (CSNPs) into chitosan/polycaprolactone (PCL) membranes. Various ratios of CSNP/PCL were prepared in the presence of constant concentration of chitosan and the films were constructed by solvent casting method. Scaffold properties including transparency, surface wettability, FTIR, and biocompatibility were examined. SEM imaging, H&E staining, and cell count were performed to investigate the HCECs adhesion. The phenotypic maintenance of the cells during culture was investigated by flow cytometry. Transparency and surface wettability improved by increasing the CSNP/PCL ratio. The CSNP/PCL 50/25, which has the lowest WCA, showed comparable transparency with human acellular corneal stroma. The scaffold was not cytotoxic and promoted the HCECs proliferation as evaluated by MTT assay. Cell counting, flow cytometry, SEM, and H&E results showed appropriate attachment of HCECs to the scaffold which formed a compact monolayer. The developed scaffold seems to be suitable for use in corneal endothelial regeneration in terms of transparency and biocompatibility. |
| first_indexed | 2024-12-14T16:03:36Z |
| format | Article |
| id | doaj.art-9a5fdf840f354daaa3bdfb742c071643 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T16:03:36Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9a5fdf840f354daaa3bdfb742c0716432022-12-21T22:55:08ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-86340-wBiofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineeringTahereh Tayebi0Alireza Baradaran-Rafii1Abbas Hajifathali2Azam Rahimpour3Hakimeh Zali4Alireza Shaabani5Hassan Niknejad6Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesOphthalmic Research Center, Department of Ophthalmology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical SciencesHematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical SciencesDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical SciencesDepartment of Polymer and Materials Chemistry, Faculty of Chemistry and Petroleum Science, Shahid Beheshti UniversityDepartment of Pharmacology, School of Medicine, Shahid Beheshti University of Medical SciencesAbstract We aimed to construct a biodegradable transparent scaffold for culturing corneal endothelial cells by incorporating chitosan nanoparticles (CSNPs) into chitosan/polycaprolactone (PCL) membranes. Various ratios of CSNP/PCL were prepared in the presence of constant concentration of chitosan and the films were constructed by solvent casting method. Scaffold properties including transparency, surface wettability, FTIR, and biocompatibility were examined. SEM imaging, H&E staining, and cell count were performed to investigate the HCECs adhesion. The phenotypic maintenance of the cells during culture was investigated by flow cytometry. Transparency and surface wettability improved by increasing the CSNP/PCL ratio. The CSNP/PCL 50/25, which has the lowest WCA, showed comparable transparency with human acellular corneal stroma. The scaffold was not cytotoxic and promoted the HCECs proliferation as evaluated by MTT assay. Cell counting, flow cytometry, SEM, and H&E results showed appropriate attachment of HCECs to the scaffold which formed a compact monolayer. The developed scaffold seems to be suitable for use in corneal endothelial regeneration in terms of transparency and biocompatibility.https://doi.org/10.1038/s41598-021-86340-w |
| spellingShingle | Tahereh Tayebi Alireza Baradaran-Rafii Abbas Hajifathali Azam Rahimpour Hakimeh Zali Alireza Shaabani Hassan Niknejad Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering Scientific Reports |
| title | Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering |
| title_full | Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering |
| title_fullStr | Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering |
| title_full_unstemmed | Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering |
| title_short | Biofabrication of chitosan/chitosan nanoparticles/polycaprolactone transparent membrane for corneal endothelial tissue engineering |
| title_sort | biofabrication of chitosan chitosan nanoparticles polycaprolactone transparent membrane for corneal endothelial tissue engineering |
| url | https://doi.org/10.1038/s41598-021-86340-w |
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