Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i>
The potato cyst nematode (PCN) <i>Globodera pallida</i> has acquired significant importance throughout Europe due to its nefarious effects on potato production. Rapid and reliable diagnosis of PCN is critical during the surveillance programs and for the implementation of control measures...
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MDPI AG
2021-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/6/744 |
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| author | Maria João Camacho Maria L. Inácio Manuel Mota Eugénia de Andrade |
| author_facet | Maria João Camacho Maria L. Inácio Manuel Mota Eugénia de Andrade |
| author_sort | Maria João Camacho |
| collection | DOAJ |
| description | The potato cyst nematode (PCN) <i>Globodera pallida</i> has acquired significant importance throughout Europe due to its nefarious effects on potato production. Rapid and reliable diagnosis of PCN is critical during the surveillance programs and for the implementation of control measures. Molecular DNA-based methods are available, but they require expensive laboratory facilities, equipment and trained technicians. Moreover, there is an additional need of time for sample shipment and testing. In this work, we have developed a new and simple assay which reliably discriminates <i>G. pallida</i> from other cyst nematodes in less than 40 min. This assay may be applied either on cysts or juveniles with the ability to detect a single juvenile of <i>G. pallida</i> in a sample of at least 40 juveniles of the non-target species <i>G. rostochiensis</i>. This test should be a tool to improve the performance of the laboratory and has the potential to be performed on-site. |
| first_indexed | 2024-03-10T10:27:34Z |
| format | Article |
| id | doaj.art-9a6252311a6845bd94e18a72df0b87ce |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T10:27:34Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-9a6252311a6845bd94e18a72df0b87ce2023-11-21T23:53:28ZengMDPI AGPathogens2076-08172021-06-0110674410.3390/pathogens10060744Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i>Maria João Camacho0Maria L. Inácio1Manuel Mota2Eugénia de Andrade3Instituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalInstituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalNemaLab-MED–Mediterranean Institute for Agriculture, Environment and Development & Department Biologia-ECT, Universidade de Évora, 7006-554 Évora, PortugalInstituto Nacional de Investigação Agrária e Veterinária (INIAV, I.P.), Quinta do Marquês, 2780-159 Oeiras, PortugalThe potato cyst nematode (PCN) <i>Globodera pallida</i> has acquired significant importance throughout Europe due to its nefarious effects on potato production. Rapid and reliable diagnosis of PCN is critical during the surveillance programs and for the implementation of control measures. Molecular DNA-based methods are available, but they require expensive laboratory facilities, equipment and trained technicians. Moreover, there is an additional need of time for sample shipment and testing. In this work, we have developed a new and simple assay which reliably discriminates <i>G. pallida</i> from other cyst nematodes in less than 40 min. This assay may be applied either on cysts or juveniles with the ability to detect a single juvenile of <i>G. pallida</i> in a sample of at least 40 juveniles of the non-target species <i>G. rostochiensis</i>. This test should be a tool to improve the performance of the laboratory and has the potential to be performed on-site.https://www.mdpi.com/2076-0817/10/6/744LAMPpotato cyst nematode<i>Globodera pallida</i> |
| spellingShingle | Maria João Camacho Maria L. Inácio Manuel Mota Eugénia de Andrade Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i> Pathogens LAMP potato cyst nematode <i>Globodera pallida</i> |
| title | Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i> |
| title_full | Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i> |
| title_fullStr | Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i> |
| title_full_unstemmed | Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i> |
| title_short | Development and Validation of a Loop-Mediated Isothermal Amplification Diagnostic Method to Detect the Quarantine Potato Pale Cyst Nematode, <i>Globodera pallida</i> |
| title_sort | development and validation of a loop mediated isothermal amplification diagnostic method to detect the quarantine potato pale cyst nematode i globodera pallida i |
| topic | LAMP potato cyst nematode <i>Globodera pallida</i> |
| url | https://www.mdpi.com/2076-0817/10/6/744 |
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